2015
DOI: 10.1021/pr501238m
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Proteomic Profiling of the Retinas in a Neonatal Rat Model of Oxygen-Induced Retinopathy with a Reproducible Ion-Current-Based MS1 Approach

Abstract: Investigation of the retina proteome during hypoxia-induced retinal neovascularization is valuable for understanding pathogenesis of retinopathy of prematurity (ROP). Here we employed a reproducible ion-current-based MS1 quantification approach (ICB) to explore the retinal proteomic changes in early stage of ROP in a rat model of oxygen-induced retinopathy (OIR). Retina proteins, which are rich in membrane proteins, were efficiently extracted by a detergent-cocktail and subjected to precipitation/on-pellet-dig… Show more

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Cited by 27 publications
(27 citation statements)
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“…Beyond defense against glaucomatous neurodegeneration by β-cystallin(s), their protective role in type 2 epithelial-to-mesenchymal transition of retinal-pigment epithelial cells occurring in dry age-related macular degeneration has been shown recently [60]. However, all crystallins were found to be upregulated in a neonatal mouse model of oxygen-induced retinopathy [61][62][63][64], which indicated a developmental stage-dependent control of their expression. Nevertheless, our data summarized in Figure 3 support the observation about a highly correlated group of α-, β-, and γ-crystallin genes [65].…”
Section: Discussionmentioning
confidence: 99%
“…Beyond defense against glaucomatous neurodegeneration by β-cystallin(s), their protective role in type 2 epithelial-to-mesenchymal transition of retinal-pigment epithelial cells occurring in dry age-related macular degeneration has been shown recently [60]. However, all crystallins were found to be upregulated in a neonatal mouse model of oxygen-induced retinopathy [61][62][63][64], which indicated a developmental stage-dependent control of their expression. Nevertheless, our data summarized in Figure 3 support the observation about a highly correlated group of α-, β-, and γ-crystallin genes [65].…”
Section: Discussionmentioning
confidence: 99%
“…In comparison, Eberhard et al ( 64 ) report the expression of Anxa1, Anxa2 and Anxa4 in reactive hippocampal astrocytes from the diseased human brain, but only detected low expression levels in normal astrocytes. Studies performed with whole retinal sample collections describe a contribution of Anxa2 and Anxa3 in neovascularization neonatal models of oxygen-induced retinopathy where they were significantly up-regulated ( 65 , 66 ). Thus, it would be highly interesting to analyze the Müller glial expression of respective annexins in similar disease models to elucidate the role of glial annexins in neoangiogenesis.…”
Section: Discussionmentioning
confidence: 99%
“…The combined effects of IH, ROS, and immature drug metabolizing enzymes can contribute to severe hepatotoxicity. Studies show that H 2 O 2 and other ROS diminish hepatic expression and activity of CYP enzymes [31–36]. …”
Section: Introductionmentioning
confidence: 99%