Proteomic response to iron deficiency in tomato root

Abstract

To know the root adjustment in response to iron deficiency, differentially displayed proteins in tomato roots of wild type and its iron uptake inefficient mutant T3238fer were analyzed by 2-DE and MALDI-TOF MS-based proteomic method under iron sufficiency and deficiency. Ninety-seven proteins were identified, 63 of them were classified in various metabolic pathways. About 40 proteins involved in starch degradation, TCA and ascorbate cycles were upregulated under iron deficiency and grouped in a network together with glycolysis, whereas proteins for fructose metabolism were decreased. Proteins involved in methionine synthesis, cell wall synthesis, mitochondria ATP synthesis, vacuole ATPase, HSP70/90, etc. also revealed enhanced expression under iron deficiency, while proteins about redox homeostasis, transcription factors, kinases, etc. showed diversified changes. The responses are closely associated with energy metabolism, organic acid formation, root morphological change, redox and sulfur homeostasis, and signal transduction, which enhance iron uptake, reutilization and other adaptive changes. Most of the proteins affected by iron deficiency and fer mutation showed similar effect on individual proteins or pathways, but the independent function of FER to iron deficiency were statistically indicated.

Quantitative Phosphoproteome Profiling of Iron-Deficient Arabidopsis Roots

“…In support of previous studies at the protein or transcript level, glycolysis appears to be activated upon Fe deficiency (Thimm et al, 2001;Brumbarova et al, 2008;Li et al, 2008Li et al, , 2009Rellán-Alvarez et al, 2010). The data from this study support this finding and add several novel aspects regarding changes of carbon flow during Fe deficiency.…”

iTRAQ Protein Profile Analysis of Arabidopsis Roots Reveals New Aspects Critical for Iron Homeostasis

“…SAM is synthesized from L-Met by S-Met adenosyltransferase (MAT). SAM synthetases are massively up-regulated at the protein level upon Fe starvation, in accordance with previous proteomic studies in tomato (Solanum lycopersicum), cucumber, and Chlamydomonas (Reinhardt et al, 2006;Li et al, 2008;Li and Schmidt, 2010). The protein abundance of three out of four Arabidopsis MAT isoenzymes, MAT1, MAT2, and MAT3, was increased by Fe deficiency.…”

Systems and Trans-System Level Analysis Identifies Conserved Iron Deficiency Responses in the Plant Lineage

“…When we compared the responses in C. reinhardtii to those documented in Arabidopsis, tomato (Solanum lycopersicum), and rice, transporters and antioxidant proteins were well represented as well, indicative of the importance of these processes throughout the plant lineage. In fact, several studies have demonstrated that iron deficiency results in enhanced antioxidant mechanisms in plants, algae, and diatoms (Zaharieva and Abadía, 2003;Allen et al, 2008;Li et al, 2008;Thamatrakoln et al, 2012). …”