Proteomic response to phosphorus deficiency and aluminum stress of three aluminum-tolerant phosphobacteria isolated from acidic soils

Barra, Patricio Javier; Duran, Paola; Delgado, Mabel; Viscardi, Sharon; Claverol, Stéphane; Larama, Giovanni; Dumont, Marc; Mora, María de la Luz

doi:10.1016/j.isci.2023.107910

Cited by 6 publications

(3 citation statements)

References 84 publications

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“…In acidic soils, characterized for toxic Al +3 levels, resident bacteria face a constant threat of Al toxicity. A recent proteomic investigation revealed an adaptation employed by Al-tolerant strains 198, RCJ4, and RJAL6 [44]. These resilient bacteria exhibit a targeted upregulation of proteins crucial for iron (Fe) uptake and trafficking upon Al³⁺ exposure.…”

Section: Secondary Metabolitesmentioning

confidence: 99%

“…The observed overproduction encompasses siderophore precursors, siderophore receptors, and Fe-siderophore transporters. This intriguing response suggests a countermeasure directed at mitigating potential disruption of intracellular Fe homeostasis, a phenomenon potentially caused by the competitive binding affinity of Al³⁺ for intracellular Fe³⁺ binding sites, leading to a putative "structural iron deficiency" [44]. Remarkably, the siderophores produced by these bacteria are versatile, and while their primary function is Fe acquisition, they readily form stable chelates with other cations like Al +3 , creating extracellular Alsiderophore complexes [10,45].…”

Section: Secondary Metabolitesmentioning

confidence: 99%

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Comparative genomics of plant growth promoting phosphobacteria isolated from acidic soils

Cortés-Albayay,

Delgado-Torres,

Larama

et al. 2024

Antonie van Leeuwenhoek

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Despite being one of the most abundant elements in soil, phosphorus (P) often becomes a limiting macronutrient for plants due to its low bioavailability, primarily locked away in insoluble organic and inorganic forms. Phosphate solubilizing and mineralizing bacteria, also called phosphobacteria, isolated from P-deficient soils have emerged as a promising biofertilizer alternative, capable of converting these recalcitrant P forms into plant-available phosphates. Three such phosphobacteria strains -Serratia sp. RJAL6, Klebsiella sp. RCJ4, and Enterobacter sp. 198previously demonstrated their particular strength as plant growth promoters for wheat, ryegrass, or avocado under abiotic stresses and P deficiency. Comparative genomic analysis of their draft genomes revealed several genes encoding key functionalities, including alkaline phosphatases, isonitrile secondary metabolites, enterobactin biosynthesis and genes associated to the production of indole-3-acetic acid (IAA) and gluconic acid. Moreover, overall genome relatedness indexes (OGRIs) revealed substantial divergence between Serratia sp. RJAL6 and its closest phylogenetic neighbors, Serratia nematodiphila and Serratia bockelmanii. This compelling evidence suggests that RJAL6 merits classification as a novel species. This in silico genomic analysis provides vital insights into the plant growth-promoting capabilities and provenance of these promising PSRB strains. Notably, it paves the way for further characterization and potential application of the newly identified Serratia species as a powerful bioinoculant in future agricultural settings.

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Section: Secondary Metabolitesmentioning

confidence: 99%

Section: Secondary Metabolitesmentioning

confidence: 99%

Comparative genomics of plant growth promoting phosphobacteria isolated from acidic soils

Cortés-Albayay,

Delgado-Torres,

Larama

et al. 2024

Antonie van Leeuwenhoek

Self Cite

View full text Add to dashboard Cite

show abstract

“…A deficiency in available phosphorus has become a common phenomenon in terrestrial ecosystems worldwide [10]. Although the total phosphorus content in the soil is high, insoluble chelates are formed by chelation with aluminum and iron in acidic soil and calcium in alkaline soil, which affects the supply of available phosphorus in the soil [11,12]. To adapt to this lower available phosphate supply in soil conditions, plants have developed phosphorus response strategies, such as changing root morphogenesis, secreting root exudates, and promoting phosphorus circulation in plants, via long-term evolution [13][14][15]; however, the mechanism is not clearly defined in ginseng.…”

Section: Introductionmentioning

confidence: 99%

Effect of Phosphate-Deficiency Stress on the Biological Characteristics and Transcriptomics of Panax ginseng

Sun,

Liang,

Shao

et al. 2024

Horticulturae

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The low availability of phosphorus has become a common problem worldwide. Phosphorus is essential for phenotypic morphology and ginsenoside synthesis. However, the effects of Pi stress on ginseng phenotype and ginsenoside synthesis remain unclear. Phenotypic analyses and transcriptomics revealed the phenotypic construction and regulation of differential genes involved in the physiological metabolism of ginseng under low-Pi stress. Root length and stem length were found to be significantly inhibited by phosphate-deficiency stress in the half-phosphate (HP) and no-phosphate (NP) treatment groups; however, the number of fibrous roots, which are regulated by phytohormones, was found to increase. In ginseng leaves, the indexes of physiological stress, superoxide anion (221.19 nmol/g) and malonaldehyde (MDA) (0.05 μmol/min/g), reached the maximum level. Moreover, chlorophyll fluorescence images and chlorophyll content further confirmed the inhibition of ginseng photosynthesis under low-Pi stress. A total of 579 and 210 differentially expressed genes (DEGs) were shared between NP and total phosphate (TP) and HP and TP, respectively, and only 64 common DEGs were found based on the two comparisons. These DEGs were mainly related to the synthesis of phosphate transporters (PHTs), phytohormones, and ginsenosides. According to KEGG analyses, four DEGs (Pg_s 0368.2, Pg_s3418.1, Pg_s5392.5 and Pg_s3342.1) affected acetyl-CoA production by regulating glycometabolism and tricarboxylic acid cycle (TCA). In addition, related genes, including those encoding 13 PHTs, 15 phytohormones, and 20 ginsenoside synthetases, were screened in ginseng roots under Pi-deficiency stress. These results indicate that changes in the ginseng phenotype and transcriptional regulation of DEGs are involved in the Pi-deficiency stress environment of ginseng, thereby providing new information regarding the development of ginseng for low-Pi tolerance.

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Plant Growth-Promotion and Abiotic Stress Tolerance of Dark Septate Endophyte Fungi Isolated from Roots of Native Andean Ericaceae Plants Colonizing Volcanic Deposits in Southern Chile

Soto,

Sanhueza,

Ortiz

et al. 2024

J Soil Sci Plant Nutr

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Proteomic response to phosphorus deficiency and aluminum stress of three aluminum-tolerant phosphobacteria isolated from acidic soils

Cited by 6 publications

References 84 publications

Comparative genomics of plant growth promoting phosphobacteria isolated from acidic soils

Comparative genomics of plant growth promoting phosphobacteria isolated from acidic soils

Effect of Phosphate-Deficiency Stress on the Biological Characteristics and Transcriptomics of Panax ginseng

Plant Growth-Promotion and Abiotic Stress Tolerance of Dark Septate Endophyte Fungi Isolated from Roots of Native Andean Ericaceae Plants Colonizing Volcanic Deposits in Southern Chile

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