Proteomic responses of fruits to environmental stresses

Chan, Zhulong

doi:10.3389/fpls.2012.00311

Cited by 25 publications

(18 citation statements)

References 97 publications

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“…The proteins present in the mesocarp of fleshy fruits tend to be associated with the metabolic processes of the plant, environment and the stress of pathogen exposure. When plants are subjected to different environmental stresses, qualitative and quantitative changes in the protein content occur [23]. This phenomenon could explain the levels of total proteins found in the pulp of the fruit of C. microphylla (4.85%), originating in the xerophytic Monte in Northern Patagonia, whose values were higher than those reported for Z. mauritiana grown in the fertile plains of Bangladesh (i.e., 0.85%-1.2%) [24].…”

Section: Analysis Of Phytonutrients In C Microphyllacontrasting

confidence: 49%

Chemical and Biological Characterization from Condalia microphylla Fruits, a Native Species of Patagonia Argentina

Boeri¹,

Piñuel²,

Sharry³

et al. 2017

JAST-B

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Condalia microphylla Cav. (Rhamnaceae), popularly known as "piquillin", is widely distributed in Patagonia. The drupes are consumed as fresh fruits by Argentine communities. The aim of this work was to quantify the nutritional value of C. microphylla fruit and the phenolic compounds present and to determine the functional antioxidant properties in vitro and in vivo. The nutritional value was determined according to the Association of Official Analytical Chemists (AOAC) methodology, and phenolic compounds were quantified by diode-array detection (HPLC-DAD). Antioxidant activity in vitro and in vivo was analyzed through the use of the radical species 2,2-diphenyl-1-picrylhydrazyl (DPPH) and zebrafish model, respectively. Quercetin-3-O-rutinoside (rutin) was the single principal phenolic compound. The extracts contained in vitro antioxidant activities and total phenolic contents (TPCs) between 1,143 ± 112 µg and 4,633 ± 174 µg gallic-acid equivalents (GAEs) per 100 g dry weight (DW), though no relationship was found between the latter parameter and the antioxidant activity of the extracts. When zebrafish larvae were exposed to oxidative stress (2.4% v/v H 2 O 2 ), a concentration as low as 1.44 µg of GAEs/mL of piquillin-derived polyphenols inhibited lipid oxidation by up to 40%. Thus, in view of these advantageous functional food properties and the opportunity to exploit this Patagonian natural resource, piquillin consumption should be promoted worldwide.

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Section: Analysis Of Phytonutrients In C Microphyllacontrasting

confidence: 49%

Chemical and Biological Characterization from Condalia microphylla Fruits, a Native Species of Patagonia Argentina

Boeri¹,

Piñuel²,

Sharry³

et al. 2017

JAST-B

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“…Compatible solutes (Lauxmann et al 2014), cell walls (Brummell et al 2004a;Gonzalez-Aguero et al 2008), carbohydrates (Nilo et al 2010;Wang et al 2013), energy substrates (Nilo et al 2010), phytohormones (Zhou et al 2001;Dagar et al 2012), transcriptional regulation (Puig et al 2015) as well as cold defense proteins (Dagar et al 2010) have been proposed to be linked with peach fruit CI. In addition, data from genome-wide analysis have suggested that comprehensive and fine metabolic adjustments as well as complicated regulation are involved in postharvest peach CI (Vizoso et al 2009;Nilo et al 2010;Chan 2013;Pons et al 2014;Pegoraro et al 2015;Puig et al 2015;Sanhueza et al 2015). However, very little is known about the early events involved in the response to cold and the regulatory mechanisms that operate at the molecular level.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and metabolic analyses provide new insights into chilling injury in peach fruit

Wang

Zhang

et al. 2017

Plant Cell & Environment

101

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Low temperature conditioning (LTC) alleviates peach fruit chilling injury but the underlying molecular basis is poorly understood. Here, changes in transcriptome, ethylene production, flesh softening, internal browning and membrane lipids were compared in fruit maintained in constant 0 °C and LTC (pre-storage at 8 °C for 5 d before storage at 0 °C). Low temperature conditioning resulted in a higher rate of ethylene production and a more rapid flesh softening as a result of higher expression of ethylene biosynthetic genes and a series of cell wall hydrolases. Reduced internal browning of fruit was observed in LTC, with lower transcript levels of polyphenol oxidase and peroxidase, but higher lipoxygenase. Low temperature conditioning fruit also showed enhanced fatty acid content, increased desaturation, higher levels of phospholipids and a preferential biosynthesis of glucosylceramide. Genes encoding cell wall hydrolases and lipid metabolism enzymes were coexpressed with differentially expressed ethylene response factors (ERFs) and contained ERF binding elements in their promoters. In conclusion, LTC is a special case of cold acclimation which increases ethylene production and, operating through ERFs, promotes both softening and changes in lipid composition and desaturation, which may modulate membrane stability, reducing browning and contributing to alleviation of peach fruit chilling injury.

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“…Recently, it was also proposed that CTG134 gene, a precursor of a peptide hormone of the RGF/GLV, regulates ethylene-auxin cross-talk during peach fruit ripening (Tadiello et al, 2016). Given that the proteome is closer to the fruit phenotype than is the genome or the transcriptome along with the fact that it is more directly responsive to environmental stimuli (Chan, 2012), proteomic analysis provides a comprehensive view of fruit's adaptation to different environments. Almost all past proteomic studies of peach fruit were focused on mesocarp tissue (Lara et al, 2009; Nilo et al, 2010, 2012; Hu et al, 2011; Prinsi et al, 2011; Zhang et al, 2011; Giraldo et al, 2012); whereas research concerning skin proeome as an isolated component during peach ripening have not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Comparative Physiological and Proteomic Analysis Reveal Distinct Regulation of Peach Skin Quality Traits by Altitude

et al. 2016

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The role of environment in fruit physiology has been established; however, knowledge regarding the effect of altitude in fruit quality traits is still lacking. Here, skin tissue quality characters were analyzed in peach fruit (cv. June Gold), harvested in 16 orchards located in low (71.5 m mean), or high (495 m mean) altitutes sites. Data indicated that soluble solids concentration and fruit firmness at commercial harvest stage were unaffected by alitute. Peach grown at high-altitude environment displayed higher levels of pigmentation and specific antioxidant-related activity in their skin at the commercial harvest stage. Skin extracts from distinct developmental stages and growing altitudes exhibited different antioxidant ability against DNA strand-scission. The effects of altitude on skin tissue were further studied using a proteomic approach. Protein expression analysis of the mature fruits depicted altered expression of 42 proteins that are mainly involved in the metabolic pathways of defense, primary metabolism, destination/storage and energy. The majority of these proteins were up-regulated at the low-altitude region. High-altitude environment increased the accumulation of several proteins, including chaperone ClpC, chaperone ClpB, pyruvate dehydrogenase E1, TCP domain class transcription factor, and lipoxygenase. We also discuss the altitude-affected protein variations, taking into account their potential role in peach ripening process. This study provides the first characterization of the peach skin proteome and helps to improve our understanding of peach's response to altitude.

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Proteomic responses of fruits to environmental stresses

Cited by 25 publications

References 97 publications

Chemical and Biological Characterization from Condalia microphylla Fruits, a Native Species of Patagonia Argentina

Chemical and Biological Characterization from Condalia microphylla Fruits, a Native Species of Patagonia Argentina

Transcriptomic and metabolic analyses provide new insights into chilling injury in peach fruit

Comparative Physiological and Proteomic Analysis Reveal Distinct Regulation of Peach Skin Quality Traits by Altitude

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