Proteomic Responses to Drought Vary Widely Among Eight Diverse Genotypes of Rice (Oryza sativa)

Hamzelou, Sara; Pascovici, Dana; Kamath, Karthik Shantharam; Amirkhani, Ardeshir; McKay, Matthew J.; Mirzaei, Mehdi; Atwell, Brian J.; Haynes, Paul A.

doi:10.3390/ijms21010363

Cited by 24 publications

(20 citation statements)

References 37 publications

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“…Proteomics, which is the study of the structural and functional features of all the proteins in an organism, is an important means of understanding complex biological mechanisms, including plant responses to abiotic stress tolerance (Deshmukh et al, 2014). Using transcriptomic or proteomics sequencing, many genes associated with drought response were identified in a number of plants, such as Arabidopsis (Cho et al, 2013; Matsui et al, 2008; Meng et al, 2019), rice (Hamzelou et al, 2020; Li et al, 2019), wheat (Fotovat et al, 2017; Kang et al, 2012; Ma et al, 2017; Michaletti et al, 2018), maize (Zeng et al, 2019; Zheng et al, 2020), soybean (Song, Prince, et al, 2016; Wang & Komatsu, 2018), tobacco (Chen et al, 2019; Khan et al, 2019), and others (An et al, 2020; Ghatak et al, 2017; Jangpromma et al, 2010; Ngara & Ndimba, 2013; Raney et al, 2014; Rodziewicz et al, 2019; Singh et al, 2017; Utsumi et al, 2012; Wisniewski et al, 2009; Yang et al, 2017). In rapeseed, numerous candidate genes associated with drought response have also been identified by transcriptomic and proteomics analyses.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and proteome analyses of the molecular mechanisms underlying changes in oil storage under drought stress in Brassica napus L.

Zhang

et al. 2021

GCB Bioenergy

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Rapeseed (Brassica napus L.) is the second most important oilseed crop in edible vegetable oil and bioenergy; however, drought stress generally causes a decrease in rapeseed yield and oil content, especially during the reproductive stage. In our study, we measured the oil and protein contents and gibberellic acid (GA) and abscisic acid (ABA) levels in seeds that were acquired on the 30th, 40th, and 50th days after flowering under control and drought treatments. RNA and protein libraries were constructed from the stressed seeds to perform transcriptome and proteome analyses, respectively. Our results demonstrated that the oil content decreased due to four primary mechanisms: downregulation of fatty acid biosynthesis‐associated genes and proteins; upregulation of fatty acid degradation‐associated genes and proteins; enhancement of protein storage due to changes in the abundances of relevant genes and proteins; and upregulation of Gly‐Asp‐Ser‐Leu (GDSL) gene expression, potentially as the result of upregulating the GA biosynthesis gene GA20ox3 and downregulating the GA inactivating gene GA2ox3 and thus an increase in GA content. During seed maturation, oil storage change may also relate to increasing ABA content as the upregulation of two members of NCED6 (9‐cis‐epoxycarotenoid dioxygenase) gene family involved in ABA biosynthesis, and the upregulation of genes involved in ABA signal transduction. These results will help to establish a foundation for breeding excellent varieties of rapeseed with high oil content for areas with frequent droughts to promote the supply of edible vegetable oil and biofuel.

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Section: Introductionmentioning

confidence: 99%

Transcriptome and proteome analyses of the molecular mechanisms underlying changes in oil storage under drought stress in Brassica napus L.

Zhang

et al. 2021

GCB Bioenergy

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“…Omics strategies have successively been used for identification of drought responsive genes, their expression controls regulatory network and the associated functional proteins and metabolic changes in plants. These studies may provide insights into the potential targets for rice improvement under drought conditions (Hamzelou et al ., 2020). Deciphering the stress responsive circuit in rice plant during drought conditions can provide a clear understanding of how rice cultivars respond to drought stress and help in developing efficient drought‐tolerant varieties to address food security raised by water scarcity.…”

Section: Physiological Mechanisms Associated With Rice Acclimation During Drought Ascendancymentioning

confidence: 99%

“…In a comparative proteome profiling of eight rice diverse genotypes (including both japonica and indica sp.) identification of an 18.6 kDa class III small HSP (HSP18.6) as a drought‐induced protein along with four isoforms of LEA proteins were detected in all cultivars (Hamzelou et al ., 2020). N22 genotype that is highly tolerant to drought stress displayed maximum accumulation of HSP18.6 and four LEA proteins confirming the pivotal roles of these proteins in drought stress tolerance (Hamzelou et al ., 2020).…”

Section: Physiological Mechanisms Associated With Rice Acclimation During Drought Ascendancymentioning

confidence: 99%

“…identification of an 18.6 kDa class III small HSP (HSP18.6) as a drought‐induced protein along with four isoforms of LEA proteins were detected in all cultivars (Hamzelou et al ., 2020). N22 genotype that is highly tolerant to drought stress displayed maximum accumulation of HSP18.6 and four LEA proteins confirming the pivotal roles of these proteins in drought stress tolerance (Hamzelou et al ., 2020). Moreover, drought stress‐induced elevation in different isoforms of LEA proteins including group 6 LEA protein, LEA type‐1 protein, LEA protein and putative HSP further confirms their role in drought tolerance in rice (Muthurajan et al ., 2011).…”

Section: Physiological Mechanisms Associated With Rice Acclimation During Drought Ascendancymentioning

confidence: 99%

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Improving drought tolerance in rice: Ensuring food security through multi‐dimensional approaches

Khan

Palakolanu

Chopra

et al. 2020

Physiologia Plantarum

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Drought has been highly prevalent around the world especially in Sub-Saharan Africa and South-East Asian countries. Consistent climatic instabilities and unpredictable rainfall patterns are further worsening the situation. Rice is a C 3 staple cereal and an important food crop for the majority of the world's population and drought stress is one of the major growth retarding threats for rice that slashes down grain quality and yield. Drought deteriorates rice productivity and induces various acclimation responses that aids in stress mitigation. However, the complexity of traits associated with drought tolerance has made the understanding of drought stress-induced responses in rice a challenging process. An integrative understanding based on physiological adaptations, omics, transgenic and molecular breeding approaches successively backed up to developing drought stress-tolerant rice. The review represents a step forward to develop drought-resilient rice plants by exploiting the knowledge that collaborates with omics-based developments with integrative efforts to ensure the compilation of all the possible strategies undertaken to develop drought stresstolerant rice. | INTRODUCTIONClimatic fluctuations in recent decades have been escalating the frequency and extremity of calamities in many parts of the world. More than 83% of the detrimental effects caused by drought occur in the agricultural sector that leads to crop loss and limited productivity, affecting food supplies and livelihood of people and has emerged as the most serious famines inducing factor across the globe (FAO, 2018). Rice is one of the major cereals and its consumption comprises over 27% of total cereal utilisation with an output of 738.2 million tons globally (FAO, 2016). Rice is a paddy field crop with more water requirement for growth and thus drought stress is considered as the major inimical that hinders rice growth, productivity and yield (Mumtaz et al., 2020).Drought stress detrimentally affects rice production by deteriorating everything from seed germination to the reproductive stages

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“…In this context, quantitative proteomic analysis identifies abundance changes in stress-related protein biomarkers [ 15 ]. In a previous study we examined drought stress response in eight different varieties of O. sativa [ 16 ]. In this study, we performed a detailed quantitative proteomic analysis of O. sativa (cv.…”

Section: Introductionmentioning

confidence: 99%

Wild and Cultivated Species of Rice Have Distinctive Proteomic Responses to Drought

Hamzelou

Kamath

Masoomi‐Aladizgeh

et al. 2020

IJMS

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Drought often compromises yield in non-irrigated crops such as rainfed rice, imperiling the communities that depend upon it as a primary food source. In this study, two cultivated species (Oryza sativa cv. Nipponbare and Oryza glaberrima cv. CG14) and an endemic, perennial Australian wild species (Oryza australiensis) were grown in soil at 40% field capacity for 7 d (drought). The hypothesis was that the natural tolerance of O. australiensis to erratic water supply would be reflected in a unique proteomic profile. Leaves from droughted plants and well-watered controls were harvested for label-free quantitative shotgun proteomics. Physiological and gene ontology analysis confirmed that O. australiensis responded uniquely to drought, with superior leaf water status and enhanced levels of photosynthetic proteins. Distinctive patterns of protein accumulation in drought were observed across the O. australiensis proteome. Photosynthetic and stress-response proteins were more abundant in drought-affected O. glaberrima than O. sativa, and were further enriched in O. australiensis. In contrast, the level of accumulation of photosynthetic proteins decreased when O. sativa underwent drought, while a narrower range of stress-responsive proteins showed increased levels of accumulation. Distinctive proteomic profiles and the accumulated levels of individual proteins with specific functions in response to drought in O. australiensis indicate the importance of this species as a source of stress tolerance genes.

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Proteomic Responses to Drought Vary Widely Among Eight Diverse Genotypes of Rice (Oryza sativa)

Cited by 24 publications

References 37 publications

Transcriptome and proteome analyses of the molecular mechanisms underlying changes in oil storage under drought stress in Brassica napus L.

Transcriptome and proteome analyses of the molecular mechanisms underlying changes in oil storage under drought stress in Brassica napus L.

Improving drought tolerance in rice: Ensuring food security through multi‐dimensional approaches

Wild and Cultivated Species of Rice Have Distinctive Proteomic Responses to Drought

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