Proteomic Studies on the Development of the Central Nervous System and Beyond

Abstract

Neuroproteomics has become a 'symbol' or even a 'sign' for neuroscientists in the post-genomic era. During the last several decades, a number of proteomic approaches have been used widely to decipher the complexity of the brain, including the study of embryonic stages of human or non-human animal brain development. The use of proteomic techniques has allowed for great scientific advancements, including the quantitative analysis of proteomic data using 2D-DIGE, ICAT and iTRAQ. In addition, proteomic studies of the brain have expanded into fields such as subproteomics, synaptoproteomics, neural plasma membrane proteomics and even mitochondrial proteomics. The rapid progress that has been made in this field will not only increase the knowledge based on the neuroproteomics of the developing brain but also help to increase the understanding of human neurological diseases. This paper will focus on proteomic studies in the central nervous system and especially those conducted on the development of the brain in order to summarize the advances in this rapidly developing field.

A multidimensional approach to an in-depth proteomics analysis of transcriptional regulators in neuroblastoma cells

“…More recently, specialized proteomic studies such as those of neuroproteomics are becoming increasingly useful for understanding the dynamic regulatory protein networks underlying neuronal development and neurological diseases (Lin et al , 2009; Liu et al , 2006; Tyler et al , 2011). In addition, neuroproteomics has branched into more in-depth studies of the sub-proteomes, including synaptoproteomics and neural plasma membrane proteomics (Zhang, 2010). However, compared with other high-throughput tools for system-wide analyses of genes and proteins, the sensitivities of proteomics technologies for the characterization of less abundant signaling molecules and transcriptional regulators have remained low for routine biochemical studies.…”

Comparison of Plasma and Cerebrospinal Fluid Proteomes Identifies Gene Products Guiding Adult Neurogenesis and Neural Differentiation in Birds

“…Using gel-based techniques and mass spectrometry, the proteomes of eCSF from rat, chicken and human have been previously described to elucidate the molecular composition of CSF and its biological role during embryonic neurogenesis [10][11][12]48 . Human eCSF contained 188 proteins, 44% of those also present in rat eCSF.…”

Alzheimer's and Parkinson's diseases: An environmental proteomic point of view

“…Proteomic approaches applied in studies of the CNS performed with embryonic and postnatal brain tissue, different brain regions, CSF, neural stem cells, pre and post synaptic proteomes and neurodegenerative diseases were extensively reviewed by Zang [23]. One very interesting and elegant investigation was carried out by Bernay and colleagues, aimed to study the secretome of the CNS; in different words, the contents of the extracellular compartment.…”