Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats

Kanamoto, Takashi; Souchelnytskyi, Nazariy; Kurimoto, Takuji; Ikeda, Yasuhiro; Sakaue, Hiroaki; Munemasa, Yasunari; Kiuchi, Yoshiaki

doi:10.1155/2015/492050

Cited by 16 publications

(11 citation statements)

References 28 publications

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“…These proteins appear to play various functions (e.g., cellular signaling, synaptic transmission, metabolism, immune function, and structural maintenance) and included several suspected retinal regeneration factors, such as epidermal growth factor receptor (EGFR), prohibitin, tenascin-X, etc. Interestingly, IGF-1, BDNF, and FGF-2 were surprisingly absent from this list, a discrepancy the authors believe is related to their experiments being conducted on wildtype retinas versus a degenerative model 135 , similar to a previous study reporting changes in the transcriptome 124 . Thus, it can be hypothesized that ES treatment may alter the secretion of these neurotrophic factors, resulting in a more balanced and less hostile environment.…”

Section: Application Of Electrical Stimulation To Enhance Cell Transpsupporting

confidence: 55%

“…In support of this theory, Zhou et al 134 found a positive regulatory effect of ES on the production of BDNF and CNTF in Mμller cells, and also provided evidence that BDNF rather than CNTF may play a major role in the promotion of Mμller cell-induced photoreceptor cell survival. Finally, in a recent proteomic study 135 , TcES performed on wild-type adult Wistar rats was shown to significantly alter the expression of 25 proteins. These proteins appear to play various functions (e.g., cellular signaling, synaptic transmission, metabolism, immune function, and structural maintenance) and included several suspected retinal regeneration factors, such as epidermal growth factor receptor (EGFR), prohibitin, tenascin-X, etc.…”

Section: Application Of Electrical Stimulation To Enhance Cell Transpmentioning

confidence: 95%

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Using Electrical Stimulation to Enhance the Efficacy of Cell Transplantation Therapies for Neurodegenerative Retinal Diseases: Concepts, Challenges, and Future Perspectives

et al. 2017

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Disease or trauma-induced loss or dysfunction of neurons in any central nervous system (CNS) tissue will have a significant impact on the health of the affected patient. The retina is a multilayered tissue that originates from the neuroectoderm, much like the brain and spinal cord. While sight is not required for life, neurodegenerationrelated loss of vision not only affects the quality of life for the patient but also has societal implications in terms of health care expenditure. Thus, it is essential to develop effective strategies to repair the retina and prevent disease symptoms. To address this need, multiple techniques have been investigated for their efficacy in treating retinal degeneration. Recent advances in cell transplantation (CT) techniques in preclinical, animal, and in vitro culture studies, including further evaluation of endogenous retinal stem cells and the differentiation of exogenous adult stem cells into various retinal cell types, suggest that this may be the most appropriate option to replace lost retinal neurons. Unfortunately, the various limitations of CT, such as immune rejection or aberrant cell behavior, have largely prevented this technique from becoming a widely used clinical treatment option. In parallel with the advances in CT methodology, the use of electrical stimulation (ES) to treat retinal degeneration has also been recently evaluated with promising results. In this review, we propose that ES could be used to enhance CT therapy, whereby electrical impulses can be applied to the retina to control both native and transplanted stem cell behavior/survival in order to circumvent the limitations associated with retinal CT. To highlight the benefits of this dual treatment, we have briefly outlined the recent developments and limitations of CT with regard to its use in the ocular environment, followed by a brief description of retinal ES, as well as described their combined use in other CNS tissues.

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Section: Application Of Electrical Stimulation To Enhance Cell Transpsupporting

confidence: 55%

Section: Application Of Electrical Stimulation To Enhance Cell Transpmentioning

confidence: 95%

Using Electrical Stimulation to Enhance the Efficacy of Cell Transplantation Therapies for Neurodegenerative Retinal Diseases: Concepts, Challenges, and Future Perspectives

et al. 2017

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“…Electrical stimulation therapy stimulates neurotrophic factors and cell survival pathways by modulating proteins (Kanamoto et al, 2015) and genes (Willmann et al, 2011) involved in cellular signaling, neuronal transmission, metabolism and inflammation (Fig. 7).…”

Section: Electrical Stimulationmentioning

confidence: 99%

Neuroprotective strategies for retinal disease

Pardue

Allen

2018

Progress in Retinal and Eye Research

188

160

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Diseases that affect the eye, including photoreceptor degeneration, diabetic retinopathy, and glaucoma, affect 11.8 million people in the US, resulting in vision loss and blindness. Loss of sight affects patient quality of life and puts an economic burden both on individuals and the greater healthcare system. Despite the urgent need for treatments, few effective options currently exist in the clinic. Here, we review research on promising neuroprotective strategies that promote neuronal survival with the potential to protect against vision loss and retinal cell death. Due to the large number of neuroprotective strategies, we restricted our review to approaches that we had direct experience with in the laboratory. We focus on drugs that target survival pathways, including bile acids like UDCA and TUDCA, steroid hormones like progesterone, therapies that target retinal dopamine, and neurotrophic factors. In addition, we review rehabilitative methods that increase endogenous repair mechanisms, including exercise and electrical stimulation therapies. For each approach, we provide background on the neuroprotective strategy, including history of use in other diseases; describe potential mechanisms of action; review the body of research performed in the retina thus far, both in animals and in humans; and discuss considerations when translating each treatment to the clinic and to the retina, including which therapies show the most promise for each retinal disease. Despite the high incidence of retinal diseases and the complexity of mechanisms involved, several promising neuroprotective treatments provide hope to prevent blindness. We discuss attractive candidates here with the goal of furthering retinal research in critical areas to rapidly translate neuroprotective strategies into the clinic.

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“…It was further proposed that the acute changes were direct and transient effects induced by electric shock to neural cells, whereas the chronic changes were indirect and secondary effects in mRNA expression or protein signaling pathways. 17 …”

Section: Improving Vision By Electrostimulationmentioning

confidence: 99%

Electrical Stimulation as a Means for Improving Vision

Sehic

Guo

Cho

et al. 2016

The American Journal of Pathology

138

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Evolving research has provided evidence that noninvasive electrical stimulation (ES) of the eye may be a promising therapy for either preserving or restoring vision in several retinal and optic nerve diseases. In this review, we focus on minimally invasive strategies for the delivery of ES and accordingly summarize the current literature on transcorneal, transorbital, and transpalpebral ES in both animal experiments and clinical studies. Various mechanisms are believed to underlie the effects of ES, including increased production of neurotrophic agents, improved chorioretinal blood circulation, and inhibition of proinflammatory cytokines. Different animal models have demonstrated favorable effects of ES on both the retina and the optic nerve. Promising effects of ES have also been demonstrated in clinical studies; however, all current studies have a lack of randomization and/or a control group (sham). There is thus a pressing need for a deeper understanding of the underlying mechanisms that govern clinical success and optimization of stimulation parameters in animal studies. In addition, such research should be followed by large, prospective, clinical studies to explore the full potential of ES. Through this review, we aim to provide insight to guide future research on ES as a potential therapy for improving vision. (Am J Pathol 2016 http://dx

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Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats

Cited by 16 publications

References 28 publications

Using Electrical Stimulation to Enhance the Efficacy of Cell Transplantation Therapies for Neurodegenerative Retinal Diseases: Concepts, Challenges, and Future Perspectives

Using Electrical Stimulation to Enhance the Efficacy of Cell Transplantation Therapies for Neurodegenerative Retinal Diseases: Concepts, Challenges, and Future Perspectives

Neuroprotective strategies for retinal disease

Electrical Stimulation as a Means for Improving Vision

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