Proteomic techniques for characterisation of mesenchymal stem cell secretome

Skalníková, Helena Kupcová

doi:10.1016/j.biochi.2013.07.015

Cited by 244 publications

(186 citation statements)

References 164 publications

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“…Only recently a more systematic and integrated approach for analysis of the MSC secretome that includes LC-MS/MS detection, antibody arrays, microarrays, and bioinformatic analysis has been employed. 7,18,19 We decided to perform an unbiased LC-MS/MS analysis of secretomes of whole MSC populations and compare these with Muse cell secretome in order to identify factors that may be associated with specific features of Muse cells. To carry out a comprehensive comparative analysis, we selected whole MSC populations from bone marrow and adipose tissue (bmMSCs and aMSCs, respectively), which share several biological features but also demonstrate peculiarities in their differentiation potential, transcriptome, proteome, and immunomodulation function.…”

Section: Introductionmentioning

confidence: 99%

The secretome of MUSE cells contains factors that may play a role in regulation of stemness, apoptosis and immunomodulation

et al. 2016

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Mesenchymal stromal cells (MSCs) are a heterogeneous population, which contain several cell phenotypes: mesenchymal stem cells, progenitor cells, fibroblasts and other type of cells. Previously, we identified unique stem cells that we named multilineage-differentiating stress enduring (Muse) cells as one to several percent of MSCs of the bone marrow, adipose tissue and dermis. Among different cell populations in MSCs, Muse cells, positive for pluripotent surface marker SSEA-3, may represent cells responsible for pluripotent-like property of MSCs, since they express pluripotency genes, able to differentiated into triploblastic cells from a single cells and are self-renewable.MSCs release biologically active factors that have profound effects on local cellular dynamics. A thorough examination of MSC secretome seems essential for understanding the physiological functions exerted by these cells in our organism and also for rational cellular therapy design. In this setting, studies on secretome of Muse cells may shed light on pathways that are associated with their specific features.Our findings evidenced that secretomes of MSCs and Muse cells contain factors that regulate extracellular matrix remodeling, ox-redox activities and immune system. Muse cells appear to secrete factors that may preserve their stem cell features, allow survival under stress conditions and may contribute to their immunomodulation capacity.In detail, the proteins belonging to protein kinase A signaling, FXR/RXR activation and LXR/RXR activation pathways may play a role in regulation of Muse stem cell features. These last 2 pathways together with proteins associated with antigen presentation pathway and coagulation system may play a role in immunomodulation.

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Section: Introductionmentioning

confidence: 99%

The secretome of MUSE cells contains factors that may play a role in regulation of stemness, apoptosis and immunomodulation

et al. 2016

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“…For our purposes, BCM can be prepared from fresh pig mandibles 10,11 . Proteomic analyses of BCM revealed the complex composition, including growth factors and constituents of the extracellular matrix 10 , also extending existing knowledge on the proteasome of whole bone 18,19 . Thus, BCM should reflect the released activity of various modifications of bone grafts in vitro.…”

Section: Introductionmentioning

confidence: 68%

Bone Conditioned Medium: Preparation and Bioassay

Caballé‐Serrano

Sawada

Filho

et al. 2015

JoVE

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Autologous bone grafts are widely used in oral and maxillofacial surgery, orthopedics, and traumatology. Autologous bone grafts not only replace missing bone, they also support the complex process of bone regeneration. This favorable behavior of autografts is attributed to the three characteristics: osteoconductivity, osteogenicity, and osteoinductivity. However, there is another aspect: Bone grafts release a myriad of molecules, including growth factors, which can target mesenchymal cells involved in bone regeneration. The paracrine properties of bone grafts can be studied in vitro by the use of bone-conditioned medium (BCM). Here we present a protocol on how to prepare bone-conditioned medium from native pig cortical bone, and bone that underwent thermal processing or demineralization. Cells can be directly exposed to BCM or seeded onto biomaterials, such as collagen membranes, previously soaked with BCM. We give examples for in vitro bioassays with mesenchymal cells on the expression of TGF-β regulated genes. The presented protocols should encourage to further reveal the paracrine effects of bone grafts during bone regeneration and open a path for translational research in the broad field of reconstructive surgery. Video LinkThe video component of this article can be found at

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“…These data showed that transplanted ADSCs might exert paracrine effects to improve erectile function in STZ-induced diabetic rats. It has been increasingly observed that transplanted MSCs might exert their therapeutic effects through secreting a variety of autocrine/paracrine factors, called the secretome, 38 or exosomes enriched with distinctive miRNA and tRNA species. 39 However, in our study, we only assessed the expression of VEGF in the corpus cavernosum.…”

Section: Discussionmentioning

confidence: 99%

137 Superparamagnetic Iron Oxide Nanoparticle Targeting of Adipose Tissue-Derived Stem Cells in Diabetes-Associated Erectile Dysfunction

Zhu¹

2017

The Journal of Sexual Medicine

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Proteomic techniques for characterisation of mesenchymal stem cell secretome

Cited by 244 publications

References 164 publications

The secretome of MUSE cells contains factors that may play a role in regulation of stemness, apoptosis and immunomodulation

The secretome of MUSE cells contains factors that may play a role in regulation of stemness, apoptosis and immunomodulation

Bone Conditioned Medium: Preparation and Bioassay

137 Superparamagnetic Iron Oxide Nanoparticle Targeting of Adipose Tissue-Derived Stem Cells in Diabetes-Associated Erectile Dysfunction

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