Proteomics: An Indispensable Tool for Novel Biomarker Identification in Melanoma

Mittal, Payal; Jain, Manish

doi:10.4172/2153-0602.1000204

Cited by 4 publications

(3 citation statements)

References 89 publications

(93 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ref. ). In this manner, students are ideally capable of generating a more holistic and comprehensive representation of the role of “information flow, exchange, and storage” in sustaining life.…”

Section: Implications For Teaching and Learningmentioning

confidence: 97%

A focus on polarity: Investigating the role of orientation cues in mediating student performance on mRNA synthesis tasks in an introductory cell and molecular biology course

Olimpo

Quijas

Quintana

2017

Biochem Molecular Bio Educ

View full text Add to dashboard Cite

The central dogma has served as a foundational model for information flow, exchange, and storage in the biological sciences for several decades. Despite its continued importance, however, recent research suggests that novices in the domain possess several misconceptions regarding the aforementioned processes, including those pertaining specifically to the formation of messenger ribonucleic acid (mRNA) transcripts. In the present study, we sought to expand upon these observations through exploration of the influence of orientation cues on students' aptitude at synthesizing mRNAs from provided deoxyribonucleic acid (DNA) template strands. Data indicated that participants (n = 45) were proficient at solving tasks of this nature when the DNA template strand and the mRNA molecule were represented in an antiparallel orientation. In contrast, participants' performance decreased significantly on items in which the mRNA was depicted in a parallel orientation relative to the DNA template strand. Furthermore, participants' Grade Point Average, self-reported confidence in understanding the transcriptional process, and spatial ability were found to mediate their performance on the mRNA synthesis tasks. Collectively, these data reaffirm the need for future research and pedagogical interventions designed to enhance students' comprehension of the central dogma in a manner that makes transparent its relevance to real-world scientific phenomena. © 2017 by The International Union of Biochemistry and Molecular Biology, 45(6):501-508, 2017.

show abstract

Section: Implications For Teaching and Learningmentioning

confidence: 97%

A focus on polarity: Investigating the role of orientation cues in mediating student performance on mRNA synthesis tasks in an introductory cell and molecular biology course

Olimpo

Quijas

Quintana

2017

Biochem Molecular Bio Educ

View full text Add to dashboard Cite

show abstract

“…In order to explore this rich information source, system biology provides frameworks tailored to each commonly known -omics data type. Melanoma-specific -omics data can be obtained from genomic [15,16] and proteomic studies [17] but also from the secretome [18] and the metabolome, respectively [19,20]. Because multiple -omics data are rarely integrated with a systems-centered approach [21], the following studies and repositories are only a starting point.…”

Section: Molecular Networkmentioning

confidence: 99%

Computational models of melanoma

Albrecht

Lucarelli

Kulms

et al. 2020

Theor Biol Med Model

View full text Add to dashboard Cite

Genes, proteins, or cells influence each other and consequently create patterns, which can be increasingly better observed by experimental biology and medicine. Thereby, descriptive methods of statistics and bioinformatics sharpen and structure our perception. However, additionally considering the interconnectivity between biological elements promises a deeper and more coherent understanding of melanoma. For instance, integrative network-based tools and well-grounded inductive in silico research reveal disease mechanisms, stratify patients, and support treatment individualization. This review gives an overview of different modeling techniques beyond statistics, shows how different strategies align with the respective medical biology, and identifies possible areas of new computational melanoma research.

show abstract

“…Crabb et al performed global quantitative proteomic analysis of UM to increase our understanding of UM metastasis processes and to identify biomarkers of UM metastasis 169 . MS-based proteomics using the untargeted MS method to discover novel protein biomarker candidates and the targeted MS mode to identify peptides of interest, has been a useful tool in melanoma research 170 .…”

Section: Application Of Ms In Cancer Biomarker Discoverymentioning

confidence: 99%

Mass spectrometry-assisted gel-based proteomics in cancer biomarker discovery: approaches and application

Huang¹,

Chen²,

He³

et al. 2017

Theranostics

View full text Add to dashboard Cite

There is a critical need for the discovery of novel biomarkers for early detection and targeted therapy of cancer, a major cause of deaths worldwide. In this respect, proteomic technologies, such as mass spectrometry (MS), enable the identification of pathologically significant proteins in various types of samples. MS is capable of high-throughput profiling of complex biological samples including blood, tissues, urine, milk, and cells. MS-assisted proteomics has contributed to the development of cancer biomarkers that may form the foundation for new clinical tests. It can also aid in elucidating the molecular mechanisms underlying cancer. In this review, we discuss MS principles and instrumentation as well as approaches in MS-based proteomics, which have been employed in the development of potential biomarkers. Furthermore, the challenges in validation of MS biomarkers for their use in clinical practice are also reviewed.

show abstract

Proteomics: An Indispensable Tool for Novel Biomarker Identification in Melanoma

Cited by 4 publications

References 89 publications

A focus on polarity: Investigating the role of orientation cues in mediating student performance on mRNA synthesis tasks in an introductory cell and molecular biology course

A focus on polarity: Investigating the role of orientation cues in mediating student performance on mRNA synthesis tasks in an introductory cell and molecular biology course

Computational models of melanoma

Mass spectrometry-assisted gel-based proteomics in cancer biomarker discovery: approaches and application

Contact Info

Product

Resources

About