Proteomics Analysis of Drought Stress-Responsive Proteins in Hippophae rhamnoides L.

Abstract

Hippophae rhamnoides L. is uniquely capable of growing well under extreme environmental conditions such as water deficit, low temperature, and high altitude. Such tolerance invokes much interest in understanding the biology of this plant species and its utilization potential. In this study, analysis of drought stress-responsive proteins in H. rhamnoides was conducted wherein greenhousegrown seedlings were subjected to drought stress. By using proteomic techniques, proteins, extracted from leaves, were analyzed using two-dimensional electrophoresis and MALDI-TOF MS. Altogether, 55 proteins exhibited changes in abundance under stress. Of these, 13 proteins were identified, including three that disappeared under drought (a putative ABC transporter ATP-binging protein, a heat shock protein HslU, and a hypothetical protein XP-515578), seven that were up-regulated (three large subunits of rubisco, a hypothetical protein DSM3645-23351, a putative acyl-CoA dehydrogenase, a nesprin-2, and a Jtype co-chaperone HSC20), and three that were only detected under drought (a probable nitrogen regulation protein (NtrX), a 4-hydroxyphenylpyruvate dioxygenase, and an unnamed protein product). These proteins may function in β-oxidation pathways in mitochondria, across membranes transport, abnormal protein removal, or prevent protein aggregation arrest, cell division, cytoskeleton stabilization, iron-sulfur cluster assembly, nitrogen metabolism regulation, and antioxidant substance biosynthesis. Four proteins (J-type co-chaperone Hsc20, a putative ABC transporter ATP-binging protein, NtrX, and HslU) were deemed as new discoveries in higher plants, and their functions were predicted either from their conserved domains or homologies to other organisms. These results provide new insights into our understanding of the mechanism of drought tolerance in plants.

Protein Profile Changes of Two Chinese Fir Genotypes under Short-term Water Deficit

“…However, in this study, interestingly the RuBisCO levels in leaves of drought-resistant strain FS43 and sensitive strain FS38 were both up-regulated following the water deprivation (Tab.1). This result is in contrast with other publications, which reported decreased levels (Vu and Gesch, 1999) or even abolition of RuBisCO, following drought stress (Reddy et al, 2004), but in agreement with contrary reports from Rizhsky (2002) and Xu (2009).Therefore, the role of RuBisCO in water deficit response needs further investigations. Functional enzymes of anti-permeation play an important role in the fir drought stress response In plants, osmoregulation is an important physiological mechanism to adapt to adversities and an effective way to tolerate water deficiency.…”

Mining of microsatellites using next generation sequencing of seabuckthorn (Hippophae rhamnoides L.) transcriptome

“…Moreover, its extensive root system aids to soil conservation (Trajkovski and Jeppsson 1999). Cascades of genes imparting adaptive nature to seabuckthorn to withstand various abiotic stresses and genes related to biosynthesis of various bioactive compounds, have generated substantial interest among researchers all over the world (Yang et al 2005;Guo et al 2007;Xu et al 2009;Ghangal et al 2012;Fatima et al 2012;Ghangal et al 2013).…”

Hippophae rhamnoides N-glycoproteome analysis: a small step towards sea buckthorn proteome mining

“…Xu et al (2008) reported drought stress responsive proteome in the seedling and leaf of sea buckthorn. A total of 55 proteins showed change in abundance in drought.…”