Proteomics analysis of human pericardial fluid

Fei, Xiang; Guo, Xuejiang; Chen, Wen; Wang, Jing; Zhou, Tao; Huang, Fuhua; Cao, Changchun; Chen, Xin

doi:10.1002/pmic.201200317

Cited by 19 publications

(26 citation statements)

References 9 publications

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“…Samples were centrifuged at 10,000 rpm for 10 min to remove cell debris. Agilent’s multiple affinity removal system (MARS-14), generally used for depletion of albumin, IgG, transferrin, heptoglobin, IgM, IgA, fibrinogen, alpha antitrypsin, apolipoprotein A1, alpha1 acid glycoprotein, alpha2 macroglobin, transthyretin, complement C3 and apolipoproteins was used to deplete the abundant proteins from CSF samples [31]. Briefly, MARS-14 cartridge was conditioned using 4 ml of Buffer-A (50 g, 1 min).…”

Section: Methodsmentioning

confidence: 99%

Chitotriosidase - a putative biomarker for sporadic amyotrophic lateral sclerosis

et al. 2013

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BackgroundPotential biomarkers to aid diagnosis and therapy need to be identified for Amyotrophic Lateral Sclerosis, a progressive motor neuronal degenerative disorder. The present study was designed to identify the factor(s) which are differentially expressed in the cerebrospinal fluid (CSF) of patients with sporadic amyotrophic lateral sclerosis (SALS; ALS-CSF), and could be associated with the pathogenesis of this disease.ResultsQuantitative mass spectrometry of ALS-CSF and control-CSF (from orthopaedic surgical patients undergoing spinal anaesthesia) samples showed upregulation of 31 proteins in the ALS-CSF, amongst which a ten-fold increase in the levels of chitotriosidase-1 (CHIT-1) was seen compared to the controls. A seventeen-fold increase in the CHIT-1 levels was detected by ELISA, while a ten-fold elevated enzyme activity was also observed. Both these results confirmed the finding of LC-MS/MS. CHIT-1 was found to be expressed by the Iba-1 immunopositive microglia.ConclusionElevated CHIT-1 levels in the ALS-CSF suggest a definitive role for the enzyme in the disease pathogenesis. Its synthesis and release from microglia into the CSF may be an aligned event of neurodegeneration. Thus, high levels of CHIT-1 signify enhanced microglial activity which may exacerbate the process of neurodegeneration. In view of the multifold increase observed in ALS-CSF, it can serve as a potential CSF biomarker for the diagnosis of SALS.

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Section: Methodsmentioning

confidence: 99%

Chitotriosidase - a putative biomarker for sporadic amyotrophic lateral sclerosis

et al. 2013

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“…Pericardial fluid may be used as a biomarker for inferring pathological alteration to the heart (11), and its accumulation can be attributed to an underlying systemic or local inflammatory process (12). Therefore, it is important to identify the association between the progression of CHF and the inflammatory response in the pericardial fluid in order to find a future treatment strategy and prognosis of HF.…”

Section: Introductionmentioning

confidence: 99%

Expression of pericardial fluid T-cells and related inflammatory cytokines in patients with chronic heart failure

Iskandar

Liu

Fei

et al. 2017

Experimental and Therapeutic Medicine

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Pericardial fluid, as a biochemical indicator of heart status, directly indicates pathological alteration to the heart. The accumulation of pericardial fluid can be attributed to an underlying systemic or local inflammatory process. However, the pericardial fluid expression of cellular surface markers, as well as several cytokines in chronic heart failure (CHF), remain unclear. In order to evaluate these issues further the pericardial fluid expression of several cytokines and the surface expression of activity markers between CHF patients and non-heart failure (NHF) patients were analyzed. The pericardial fluid expression of cytokines was measured by immunofluorescence and biomarker of plasma N-terminal propeptide of B-type natriuretic peptide (NT-proBNP), while pericardial fluid levels of soluble glycoprotein 130 (sgp130) were analyzed by ELISA in 50 CHF and 24 NHF patients. In addition, the surface expression of activation markers for T-cells was measured by immunohistochemistry. Patients with CHF demonstrated increased levels of plasma NT-proBNP and pericardial fluid sgp130. Surface expression of cellular activation markers CD25 and Foxp3 in the pericardial fluid was increased in patients with CHF. Moreover, the pro- and anti-inflammatory cytokines interferon (IFN)-γ, interleukin (IL)-6 and IL-10 in patients with CHF also demonstrated an increased expression within its pericardial fluid. In addition, there was infiltration of inflammatory cells and enhanced expression of inflammatory cytokines in the pericardial fluid of patients with CHF, which may reflect T cell activation, suggesting that systemic inflammation is important in the progression of CHF. This evidence could indicate a possible novel target for future therapeutics and prevention of CHF.

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“…The plasma itself displays a range higher than 10 10 -fold [4]. Third, data integration will be difficult since we have available only one study published so far, reporting the first in-depth proteomic characterization of PF [5]. In that study the first 1000 PF proteins were identified by removing the 14 most abundant proteins (using Agilent's Multiple Affinity Removal System, MARS) and by following a SDS-PAGE-LC-MS/MS approach.…”

Section: Introductionmentioning

confidence: 99%

A fractionation approach applying chelating magnetic nanoparticles to characterize pericardial fluid's proteome

Trindade¹,

Bastos²,

Leite‐Moreira³

et al. 2017

Archives of Biochemistry and Biophysics

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Owing to their close proximity, pericardial fluid (PF)'s proteome may mirror the pathophysiological status of the heart. Despite this diagnosis potential, the knowledge of PF's proteome is scarce. Large amounts of albumin hamper the characterization of the least abundant proteins in PF. Aiming to expand PF's proteome and to validate the technique for future applications, we have fractionated and characterized the PF, using N-(trimethoxysilylpropyl)ethylenediamine triacetic acid (EDTA)-functionalized magnetic nanoparticles (NPs@EDTA) followed by a GeLC-MS/MS approach. Similarly to an albumin-depletion kit, NPs@EDTA-based fractionation was efficient in removing albumin. Both methods displayed comparable inter-individual variability, but NPs@EDTA outperformed the former with regard to the protein dynamic range as well as to the monitoring of biological processes. Overall, 565 proteins were identified, of which 297 (>50%) have never been assigned to PF. Moreover, owing to this method's good proteome reproducibility, affordability, rapid automation and high binding ability of NP@EDTA, it bears a great potential towards future clinical application.

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Proteomics analysis of human pericardial fluid

Cited by 19 publications

References 9 publications

Chitotriosidase - a putative biomarker for sporadic amyotrophic lateral sclerosis

Chitotriosidase - a putative biomarker for sporadic amyotrophic lateral sclerosis

Expression of pericardial fluid T-cells and related inflammatory cytokines in patients with chronic heart failure

A fractionation approach applying chelating magnetic nanoparticles to characterize pericardial fluid's proteome

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