Proteomics approach to understand bacterial antibiotic resistance strategies

Peng, Bo; Li, Hui; Peng, Xuan‐xian

doi:10.1080/14789450.2019.1681978

Cited by 48 publications

(34 citation statements)

References 84 publications

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“…However, with a wide use of cephalosporins in clinics, resistance to cephalosporins including ceftazidime is predominant. Inactivation of β-lactams by β-lactamases, failure in binding to penicillin-binding proteins, and alteration of binding affinity to penicillin-binding proteins are identified as the three common mechanisms of resistance to β-lactams ( Peng et al, 2019 ). However, whether ROS play a role in ceftazidime sensitivity and resistance is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Reactive Oxygen Species-Related Ceftazidime Resistance Is Caused by the Pyruvate Cycle Perturbation and Reverted by Fe3 + in Edwardsiella tarda

Peng

et al. 2021

Front. Microbiol.

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Reactive oxygen species (ROS) are related to antibiotic resistance and have been reported in bacteria. However, whether ROS contribute to ceftazidime resistance and plays a role in ceftazidime-mediated killing is unknown. The present study showed lower ROS production in ceftazidime-resistant Edwardsiella tarda (LTB4-RCAZ) than that in LTB4-sensitive E. tarda (LTB4-S), two isogenic E. tarda LTB4 strains, which was related to bacterial viability in the presence of ceftazidime. Consistently, ROS promoter Fe3+ and inhibitor thiourea elevated and reduced the ceftazidime-mediated killing, respectively. Further investigation indicated that the reduction of ROS is related to inactivation of the pyruvate cycle, which provides sources for ROS biosynthesis, but not superoxide dismutase (SOD) and catalase (CAT), which degrade ROS. Interestingly, Fe3+ promoted the P cycle, increased ROS biosynthesis, and thereby promoted ceftazidime-mediated killing. The Fe3+-induced potentiation is generalizable to cephalosporins and clinically isolated multidrug-resistant pathogens. These results show that ROS play a role in bacterial resistance and sensitivity to ceftazidime. More importantly, the present study reveals a previously unknown mechanism that Fe3+ elevates ROS production via promoting the P cycle.

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Section: Introductionmentioning

confidence: 99%

Reactive Oxygen Species-Related Ceftazidime Resistance Is Caused by the Pyruvate Cycle Perturbation and Reverted by Fe3 + in Edwardsiella tarda

Peng

et al. 2021

Front. Microbiol.

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“…Proteomics techniques have the potential to significantly contribute to increase the knowledge about molecular mechanisms related to antibiotic resistance [104]. In the last five years, metagenomics and metaproteomics have been applied to identify correlations between the "resistome" (the antibiotic immunity genes/proteins) and the transmission of ARG/Ps from natural microflora to human pathogenic microorganisms, which could become a serious health issue.…”

Section: Antibiotic Resistance In Aquaculturementioning

confidence: 99%

“…Finally, "reprogramming proteomics" needs to be developed in a general manner to revert an antibiotic-resistance proteome to an antibiotic-sensitive proteome for the control of antibiotic-resistant pathogens [104].…”

Section: Antibiotic Resistance In Aquaculturementioning

confidence: 99%

Proteomic Strategies to Evaluate the Impact of Farming Conditions on Food Quality and Safety in Aquaculture Products

Carrera

Piñeiro

Martı́nez

2020

Foods

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This review presents the primary applications of various proteomic strategies to evaluate the impact of farming conditions on food quality and safety in aquaculture products. Aquaculture is a quickly growing sector that represents 47% of total fish production. Food quality, dietary management, fish welfare, the stress response, food safety, and antibiotic resistance, which are covered by this review, are among the primary topics in which proteomic techniques and strategies are being successfully applied. The review concludes by outlining future directions and potential perspectives.

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“…The emergence of multiple antibiotic resistance capacities in several pathogens is of major concern, and proteomics could also play a pivotal role in advancing our understanding of this process and how resistance spreads, while also contributing to the search for alternative drugs [22]. The power of proteomics could be harnessed to stratify patients, as demonstrated recently with Covid-19 infections [23], to identify biomarkers of exposure (to B or C threats), or to better understand the efficacy of vaccines and accelerate their development, as illustrated for the Lassa virus, an Arena virus endemic to parts of Western Africa [24].…”

Section: Improved Characterization Of Pathogens Their Reservoirs Thmentioning

confidence: 99%

The proteomics contribution to the counter-bioterrorism toolbox in the post-COVID-19 era

Armengaud

2020

Expert Review of Proteomics

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Proteomics approach to understand bacterial antibiotic resistance strategies

Cited by 48 publications

References 84 publications

Reactive Oxygen Species-Related Ceftazidime Resistance Is Caused by the Pyruvate Cycle Perturbation and Reverted by Fe3 + in Edwardsiella tarda

Reactive Oxygen Species-Related Ceftazidime Resistance Is Caused by the Pyruvate Cycle Perturbation and Reverted by Fe3 + in Edwardsiella tarda

Proteomic Strategies to Evaluate the Impact of Farming Conditions on Food Quality and Safety in Aquaculture Products

The proteomics contribution to the counter-bioterrorism toolbox in the post-COVID-19 era

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