Proteomics: methodologies and applications to the study of human diseases

Barbosa, Eduardo Buzolin; Vidotto, Alessandra; Polachini, Giovana Mussi; Henrique, Tiago; Marqui, Alessandra Bernadete Trovó de; Tajara, Eloíza H.

doi:10.1016/s0104-4230(12)70209-9

Cited by 15 publications

(16 citation statements)

References 138 publications

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“…Proteomic studies include protein identification, quantification or localization, post-translational modification, and protein-protein interactions [ 163 ]. Liquid chromatography is a typical analyzing tool for proteomics with coupling of mass spectrometry (MS) [ 164 ]. Several studies have performed proteomic analysis and identified different protein profiles of asthmatics in serum [ 165 ], sputum [ 166 , 167 ], BALF [ 168 , 169 , 170 , 171 ], or bronchial biopsy [ 172 ] ( Table 3 ).…”

Section: Identification Of “Omics” Markers In Asthmamentioning

confidence: 99%

Potential Metabolic Biomarkers in Adult Asthmatics

2021

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Asthma is the most common chronic airway inflammation, with multiple phenotypes caused by complicated interactions of genetic, epigenetic, and environmental factors. To date, various determinants have been suggested for asthma pathogenesis by a new technology termed omics, including genomics, transcriptomics, proteomics, and metabolomics. In particular, the systematic analysis of all metabolites in a biological system, such as carbohydrates, amino acids, and lipids, has helped identify a novel pathway related to complex diseases. These metabolites are involved in the regulation of hypermethylation, response to hypoxia, and immune reactions in the pathogenesis of asthma. Among them, lipid metabolism has been suggested to be related to lung dysfunction in mild-to-moderate asthma. Sphingolipid metabolites are an important mediator contributing to airway inflammation in obese asthma and aspirin-exacerbated respiratory disease. Although how these molecular variants impact the disease has not been completely determined, identification of new causative factors may possibly lead to more-personalized and precise pathway-specific approaches for better diagnosis and treatment of asthma. In this review, perspectives of metabolites related to asthma and clinical implications have been highlighted according to various phenotypes.

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Section: Identification Of “Omics” Markers In Asthmamentioning

confidence: 99%

Potential Metabolic Biomarkers in Adult Asthmatics

2021

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“…Almost all biologic fluids (serum, plasma, urine, saliva, cerebrospinal, synovial, bronchoalveolar lavage, and amniotic fluids (AF), as well as tissue samples have been analyzed in search of specific target proteins that may act as possible diagnostic or therapeutic markers. Almost all fields of clinical medicine have profited from the characterization of proteins and their subproteomic profiles, such as the phosphoproteomes, in different tissues and medical conditions, although their use in everyday clinical practice remains a challenge (29)(30)(31).…”

Section: Tools Used In Proteomics Analysismentioning

confidence: 99%

The Use of Proteomics in Assisted Reproduction

Kosteria

Anagnostopoulos

Kanaka‐Gantenbein

et al. 2017

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“…Thus, the study of proteins is important in understanding the biological mechanisms of cells 14 . Many of the techniques employed in proteomics focus on the identification of biomarkers 15 . Three main components of proteomic method comprise separation of intricate protein mixture, analysis of separated components and identification of single protein.…”

Section: Introductionmentioning

confidence: 99%

Identification of potential canine mammary tumour cell biomarkers using proteomic approach: Differences in protein profiles among tumour and normal mammary epithelial cells by two‐dimensional electrophoresis‐based mass spectrometry

Fhaikrue

Srisawat

Nambooppha

et al. 2020

Vet Comparative Oncology

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Canine mammary tumours (CMTs) are regarded as invasive with a high rate of recurrent and metastasis in intact female dogs. Tumour diagnosis, therefore, is an important step in predicting and monitoring tumour progression. This study was designed to identify protein expression on CMTs by employing a proteomic approach. The primary cell culture from benign mixed tumour, simple carcinoma, complex carcinoma and normal mammary gland were established, and two-dimensional electrophoresis (2DE) was subsequently performed. The different spots on each sample type were collected for identification using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results indicated that cytokeratin 5 (CK5) and transketolase (TKT) were identified in benign mixed tumour cells and complex carcinoma cells. In contrast, cytokeratin 18 (CK18) and pyruvate kinase PKM were identified in simple carcinoma cells. Moreover, alpha-2-HS-glycoprotein tumour antigen was identified specifically in complex carcinoma cells. In addition, ATP-dependent 6-phosphofructokinase platelet type and elongation factor 2 proteins were observed in benign cells. In conclusion, all expressed proteins in this study have been recognized for acting as their expression that differs from healthy mammary epithelial cells. Expectantly, this study identified the expressed proteins that might be useful in further diagnostic biomarker studies on CMTs.

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Proteomics: methodologies and applications to the study of human diseases

Cited by 15 publications

References 138 publications

Potential Metabolic Biomarkers in Adult Asthmatics

Potential Metabolic Biomarkers in Adult Asthmatics

The Use of Proteomics in Assisted Reproduction

Identification of potential canine mammary tumour cell biomarkers using proteomic approach: Differences in protein profiles among tumour and normal mammary epithelial cells by two‐dimensional electrophoresis‐based mass spectrometry

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