Proteomics of Atelectatic Versus Normally Aerated Lung in Early Endotoxemic Lung Injury

Rashid, Asif; Zeng, Cheng; Ribeiro, Gabriel; Hinoshita, T.; Lessa, Marcos Adriano; Libermann, Towia A.; Dillon, Simon T.; Kosour, Carolina; Melo, Marcos F. Vidal

doi:10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a4101

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“…For the lung, pulmonary edema and pulmonary hemorrhage may occur under the action of a large number of reactive oxygen species (ROS) in the early stage (Liu et al, 2018a). Acute lung injury (ALI) symptoms such as atelectasis and atelectasis can later progress to pulmonary interstitial fibrosis (Wang et al, 2019;Rashid et al, 2020). Respiratory failure caused by the two is the main cause of death (Bi et al, 2020;Johnson et al, 2020).…”

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confidence: 99%

Paraquat Induces Lung Injury via miR-199-Mediated SET in a Mouse Model

et al. 2022

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Objective: To explore the molecular mechanism of lung injury caused by paraquat (PQ) poisoning by investigating miR-199-mediated SET.Methods: A paraquat poisoning model was established in C57BL/6 male mice via intraperitoneal injection of paraquat. The mice were transfected with miR-199 siRNA and or mimic. After 14 days of treatment, pathophysiological changes of the lung were observed and lung tissue was analyzed via Hematoxylin-Eosin staining. The levels of miR-199, SETs, surfactant protein SP-A and SP-B, and inflammatory and oxidative factors were analyzed by qPCR, Western Blot, and ELISA kits.Results: A acute lung-injury (ALI) model was established using PQ treatment and confirmed with edema of pulmonary endothelium with low electronic density of endothelial cytoplasm, presence of protein-rich fluid, and numerous erythrocytes in alveolar space, concentric figures of damaged tubular myelin, alveolar destruction, and increase in inflammatory cell numbers. Compared with the control group, miR-199 and SET levels were reduced in the PQ-treated group. miR-199 siRNA increased the SET level, inflammatory and oxidative levels, and reduced the levels of SP-A and SP-B, and miR-199 mimic reduced the SET level, inflammatory and oxidative levels, and increased the levels of SP-A and SP-B. PQ treatment reduced miR-199 level.Conclusion: Paraquat induces ALI by affecting miR-199-mediated SET.

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