Proteomics of Biotrophic Plant–Microbe Interactions: Symbioses Lead the March

Recorbet, Ghislaine; Dumas‐Gaudot, Eliane

doi:10.1002/9780470369630.ch42

Cited by 3 publications

(3 citation statements)

References 60 publications

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“…Recorbet et al [29] showed a drastic decrease in Escherichia coli target DNA sequences for 5 days after addition of E. coli cells to soil, followed by persistence of the remaining target sequences for 40 days. E. coli cells could not be detected after 14 days using selective plating and immunofluorescence techniques [29]. This indicated that possibly extracellular DNA persisted in their soil.…”

Section: Discussionmentioning

confidence: 97%

Quantitative 16S rDNA-targeted polymerase chain reaction and oligonucleotide hybridization for the detection of Paenibacillus azotofixans in soil and the wheat rhizosphere

Rosado¹

1996

FEMS Microbiology Ecology

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A molecular method for the detection of Puenibacillus a~otofxuns in soil and the wheat rhizosphere was developed. The system consisted of polymerase chain reaction (PCR) amplification of part of the variable VI to V4 regions of the 16s ribosomal RNA gene, followed by hybridization with a specific oligonucleotide probe homologous to part of the intervening region. In vitro specificity tests showed that the detection system worked specifically for P. az~tofixun~ strains, and did not detect other Puenibucillus species or species of other bacterial genera. Vegetative cells of a rifampicin resistant P. u~~r~firan~ derivative were trackable in Flevo silt loam (FSL) soil in 24 h experiments using both selective plating and most probable number (MPN)-PCR combined with probing, and plate counts parallelled MPN-PCR estimations of numbers of specific targets. MPN-PCR allowed for the detection of down to 10' introduced cells per g of dry soil. Introduced P. u~ot~fixans spores did not form colonies on selective plates, but were detectable via PCR. The P. ux~tof+wns populations introduced into the silt loam soil suffered a slow decline of the detectable plate count over a period of 14 days. MPN-PCR revealed a similar decline of the number of specific DNA targets. Greater numbers of targets were found in wheat rhizosphere from Flevo silt loam soil, and these numbers persisted throughout the experiment. Soil drying resulted in enhanced persistence of the target sequences, whereas in a constantly moist soil the numbers of target sequences declined. Rewetting of dried soil resulted in declining target sequence numbers. The MPN-PCR detection method is adequate to assess the impact of stress conditions affecting P. u~orofxuns in FSL and probably other soils, since it abolishes the need for culturing or specific markers and is direct and unambiguous due to its high specificity.

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Section: Discussionmentioning

confidence: 97%

Quantitative 16S rDNA-targeted polymerase chain reaction and oligonucleotide hybridization for the detection of Paenibacillus azotofixans in soil and the wheat rhizosphere

Rosado¹

1996

FEMS Microbiology Ecology

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“…The first proteomic investigation of the plant-mycorrhizal fungus symbiosis was published by Dumas et al (1990) and used mono-dimensional polyacrylamide gel electrophoresis (PAGE) to separate soluble proteins from non-mycorrhizal roots and from roots infected by different AM fungi [30]. After this pioneering study, and because of improvements in sample extraction, sample purification and in the technological performance of the equipment, many studies have aimed to identify the key players involved in mycorrhizal interactions ( Figure 3).…”

Section: Proteomics In Actionmentioning

confidence: 99%

“…After this pioneering study, and because of improvements in sample extraction, sample purification and in the technological performance of the equipment, many studies have aimed to identify the key players involved in mycorrhizal interactions ( Figure 3). Many strategies have been set up, depending on the target mycorrhizal type, on the symbiotic stage of interest and subcellular localization [30].…”

Section: Proteomics In Actionmentioning

confidence: 99%

Symbiotic Proteomics — State of the Art in Plant–Mycorrhizal Fungi Interactions

Chiapello¹,

Perotto²,

Balestrini³

2015

Recent Advances in Proteomics Research

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Mycorrhizae are symbiotic associations between soil fungi belonging to diverse taxa and the roots of about % of all terrestrial plant species. The mutualistic nature of these symbioses is based on the nutritional exchanges between the partners. However, the benefits to the plant partner are not limited to an improved mineral nutrition because they also include a general increase in stress tolerance and health. Because of these benefits, mycorrhizae are of great interest in sustainable agriculture and forestry. In the past few years, the development of highthroughput molecular tools, in addition to the advancements in microscopy techniques, has allowed us to gain a deeper insight on the molecular mechanisms underlying the establishment and functioning of these symbioses. In this chapter, we focus on the use of proteomic tools to better understand the molecular bases of cell communication and the regulation of developmental and metabolic pathways in mycorrhizal associations.

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Arbuscular mycorrhizal symbiosis elicits proteome responses opposite of P-starvation in SO4 grapevine rootstock upon root colonisation with two Glomus species

Cangahuala-Inocente

Silva²,

Johnson

et al. 2011

Mycorrhiza

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Although plant biotisation with arbuscular mycorrhizal fungi (AMF) is a promising strategy for improving plant health, a better knowledge regarding the molecular mechanisms involved is required. In this context, we sought to analyse the root proteome of grapevine rootstock Selection Oppenheim 4 (SO4) upon colonisation with two AMF. As expected, AMF colonisation stimulates plant biomass. At the proteome level, changes in protein amounts due to AMF colonisation resulted in 39 differentially accumulated two-dimensional electrophoresis spots in AMF roots relative to control. Out of them, 25 were co-identified in SO4 roots upon colonisation by Glomus irregulare and Glomus mosseae supporting the existence of conserved plant responses to AM symbiosis in a woody perennial species. Among the 18 proteins whose amount was reduced in AMF-colonised roots were proteins involved in glycolysis, protein synthesis and fate, defence and cell rescue, ethylene biosynthesis and purine and pyrimidine salvage degradation. The six co-identified proteins whose amount was increased had functions in energy production, signalling, protein synthesis and fate including proteases. Altogether these data confirmed that a part of the accommodation program of AMF previously characterized in annual plants is maintained within roots of the SO4 rootstock cuttings. Nonetheless, particular responses also occurred involving proteins of carbon metabolism, development and root architecture, defence and cell rescue, anthocyanin biosynthesis and P remobilization, previously reported as induced upon P-starvation. This suggests the occurrence of P reprioritization upon AMF colonization in a woody perennial plant species with agronomical interest.

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Proteomics of Biotrophic Plant–Microbe Interactions: Symbioses Lead the March

Cited by 3 publications

References 60 publications

Quantitative 16S rDNA-targeted polymerase chain reaction and oligonucleotide hybridization for the detection of Paenibacillus azotofixans in soil and the wheat rhizosphere

Quantitative 16S rDNA-targeted polymerase chain reaction and oligonucleotide hybridization for the detection of Paenibacillus azotofixans in soil and the wheat rhizosphere

Symbiotic Proteomics — State of the Art in Plant–Mycorrhizal Fungi Interactions

Arbuscular mycorrhizal symbiosis elicits proteome responses opposite of P-starvation in SO4 grapevine rootstock upon root colonisation with two Glomus species

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