Proteomics of immune cells from liver tumors reveals immunotherapy targets

Effects of combined radiotherapy with immune checkpoint blockade on immunological memory in luminal-like subtype murine bladder cancer model

“…Canale et al have demonstrated by performing mass spectrometry on liver tumors that the tumor-infiltrating CD8 T cells upregulate AFAP1L2 in response to chronic stimulation, and the deletion of AFAP1L2 in CD8 T cells enhanced their anti-tumor activity. 44 Another group had also characterized the two main subtypes of solid cancer (small squamous carcinoma and adenocarcinoma) and found distinct proteomes that provide insights on the different tumor development and therapeutic strategies. 45 Groeneveld et al studied the proteomics of 63 NMIBC and MIBC tumors and were able to find similar classification with the transcriptomics subtypes, but also found heterogeneity within the subtypes.…”

Longitudinal proteomic profiling of T cell differentiationin vivounveils dynamic proteome remodeling

“…Previous studies of T cell differentiation were performed in vitro , often applied non-antigen specific stimulation, and were mainly focused on the early stages of T cell activation 19, 20, 21 . Other studies aimed at profiling the proteome of human immune cells in the context of viral infection or cancer; and again demonstrated that proteomic profiling highlighted proteins that were overlooked in transcriptomic data sets that have potential as immunotherapeutic targets 22, 23 . Thus, comprehensive longitudinal proteomic profiling of ex-vivo CD8 T cells in a well-established model is needed to dissect these aspects of molecular alterations during T cell differentiation.…”