Proteomics of Nasal Mucus in Chronic Rhinosinusitis

Tewfik, Marc A.; Latterich, Martin; DiFalco, Marcos R.; Samaha, Mark

doi:10.2500/ajr.2007.21.3103

Cited by 48 publications

(54 citation statements)

References 28 publications

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“…Proteomic analysis showed that MUC5B was significantly overexpressed in CRS samples, whereas MUC5AC did not reach statistical significance. In line with our results, MUC5B, but not MUC5AC, has been identified by proteomic analyses of nasal mucus from CRS patients with nasal polyps (29).…”

Section: Mucins In Sinonasal Secretionssupporting

confidence: 91%

“…Each has been identified in CRS samples. S100A9 is overexpressed in nasal secretory cells from CRS patients with nasal polyps (28), as well as nasal mucus (29) and nasal lavage fluid from CRS patients (19). S100A8 is overexpressed in sinonasal mucosa tissue from patients with CRS with nasal polyps (30,31).…”

Section: Discussionmentioning

confidence: 99%

“…Proteomic studies have shown overexpression of LTF, an antimicrobial protein, in the nasal lavage fluid of patients with acute sinusitis (19), asthmatic CRS (11), smokers (10), and seasonal allergic rhinitis patients (32), as well as nasal mucus and tissue from CRS patients (29,33). MYH9, which aids in cell motility and maintains cell shape (34), and IGHA1, which acts in immune system defense (35), also exhibit increased levels in proteomic studies on nasal lavage fluid from acute sinusitis (8) and asthmatic CRS (11) patients.…”

Section: Discussionmentioning

confidence: 99%

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Proteomic analysis of pediatric sinonasal secretions shows increased MUC5B mucin in CRS

et al. 2014

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Background: Chronic rhinosinusitis (CRS) is characterized by mucous overproduction and submucosal gland hyperplasia. The global protein profile of sinonasal secretions in pediatric CRS has not been studied. We hypothesized that MUC5B, a glandular mucin, would be relatively increased in CRS secretions compared to other mucins. Methods: Secretions were collected at Children's National Health System (Children's National) from CRS patients undergoing sinus surgery and from control patients without CRS undergoing craniofacial procedures. Proteins were extracted, digested to peptides, and analyzed by mass spectometry. Fold change significance was calculated using the QSpec algorithm. Western blot analysis was performed to validate proteomic findings. results: In total, 294 proteins were identified. Although both MUC5B and MUC5AC were identified in a majority of samples, the relative abundance of MUC5B was found to be significantly higher (P < 0.05). Western blot data validated these findings. Other proteins with the highest significant positive-fold change in CRS samples were BP1 fold-containing family A member 1, chitinase-3-like protein 1, plastin-2, serpin 10, and BP1 fold-containing family B member 1. conclusion: Overall, our data demonstrate an increase of MUC5B abundance in the sinus secretions of pediatric patients with CRS.

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Section: Mucins In Sinonasal Secretionssupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Proteomic analysis of pediatric sinonasal secretions shows increased MUC5B mucin in CRS

et al. 2014

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“…This protein was also found to be upregulated in nasal mucus in a study of patients with chronic sinusitis. 12 Cystatin-S was upregulated in the symptomatic group at 20 min and in the asymptomatic group at 5 h. By contrast, in another study, lower levels of this protein were found in nasal fluids from persons suffering from seasonal allergic rhinitis compared to controls. 11 The levels of cystatin-SN in the asymptomatic group were lower than in the other two groups.…”

Section: Discussionmentioning

confidence: 80%

Time-Dependent Proteomic iTRAQ Analysis of Nasal Lavage of Hairdressers Challenged by Persulfate

et al. 2010

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Hairdressers are frequently exposed to bleaching powder containing persulfates, a group of compounds that may induce hypersensitivity in the airways. The mechanism causing this reaction is not clear. The aim of this study was to identify changes in the nasal lavage fluid proteome after challenge with potassium persulfate in hairdressers with bleaching powder-associated rhinitis. Furthermore, we aimed to compare their response to that of hairdressers without nasal symptoms, and atopic subjects with pollen-associated nasal symptoms. To study the pathogenesis of persulfate-associated rhinitis, the response in protein expression from the upper airway was assessed by time-dependent proteomic expression analysis of nasal lavage fluids. Samples were prepared by pooling nasal lavage fluids from the groups at different time points after challenge. Samples were depleted of high-abundant proteins, labeled with iTRAQ and analyzed by online 2D-nanoLC-MS/MS. Differences in the protein pattern between the three groups were observed. Most proteins with differentially expressed levels were involved in pathways of lipid transportation and antimicrobial activities. The major finding was increased abundance of apolipoprotein A-1, 20 min postchallenge, detected solely in the group of symptomatic hairdressers. Our results suggest there may be differences between the mechanisms responsible for the rhinitis in the symptomatic and atopic group.

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“…In recent years, there has been an interest in identifying human nasal mucus and fluid-phase proteins using proteomics, particularly in relation with chronic rhinosinusitis and allergic rhinitis [43,44,45,46,47,48]. These studies focus on the protein profiling of diseases with the aim of determining biomarkers in nasal secretions.…”

Section: Discussionmentioning

confidence: 99%

Surfactant Proteins A, B, C and D in the Human Nasal Airway: Associated with Mucosal Glands and Ciliated Epithelium but Absent in Fluid-Phase Secretions and Mucus

Gaunsbaek

Kjeldsen

Svane-Knudsen

et al. 2014

ORL

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Aims: To investigate the presence of surfactant protein (SP) A, B, C and D in nasal airways and to determine whether the proteins exert their main functions in nasal secretions or in the deeper layers of the nasal mucosa. Methods: Volunteers were recruited from the Department of ENT Head and Neck Surgery, Odense University Hospital, Denmark. The study included 39 subjects. Nasal mucosal biopsies were analyzed by immunohistochemistry, and bronchoalveolar and nasal lavages, nasal brush biopsies and nasal mucus were analyzed for SP-A, -B, -C and -D by SDS-PAGE and Western blotting. The presence of SP-A and SP-D in the first three samplings were also analyzed by enzyme-linked immunosorbent assay. Results: In nasal mucosal biopsies, SP-A, -B, -C and -D were all demonstrated in the serous acini of the submucosal glands and in the surface epithelium. SP-D was detected in nasal brush biopsies, whereas the other SPs were absent. Moreover, SP-A, -B, -C and -D were absent in nasal lavage and mucus. Conclusion: SP-A, -B, -C and -D exert their protective effect in the ductal epithelium of the submucosal glands rather than in nasal secretions and mucus. Further studies are required to clarify the functions of these proteins in nasal secretory pathways for understanding upper airway diseases. i 2014 S. Karger AG, Basel

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Proteomics of Nasal Mucus in Chronic Rhinosinusitis

Abstract: The field of proteomics has great potential in leading to a better understanding of the mechanism of the disease process in CRS. Differences in the expression of proteins related to regulation of immune cells and mediators merit additional investigation.

Cited by 48 publications

References 28 publications

Proteomic analysis of pediatric sinonasal secretions shows increased MUC5B mucin in CRS

Proteomic analysis of pediatric sinonasal secretions shows increased MUC5B mucin in CRS

Time-Dependent Proteomic iTRAQ Analysis of Nasal Lavage of Hairdressers Challenged by Persulfate

Surfactant Proteins A, B, C and D in the Human Nasal Airway: Associated with Mucosal Glands and Ciliated Epithelium but Absent in Fluid-Phase Secretions and Mucus

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