Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

Huang, Xiaoyan; Sha, Jiahao

doi:10.1038/aja.2010.71

Cited by 30 publications

(20 citation statements)

References 62 publications

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“…We reported protein expression of germ cells at different developmental stages, including meiotic and haploid germ cells, thereby identifying the proteomic profiles of cells associated with spermatogenesis (Guo et al 2010b(Guo et al , 2011. These data have helped to develop our understanding of the regulation of male fertility (Huang & Sha 2011). Several proteins reported in these studies were selected for further investigation.…”

Section: Introductionmentioning

confidence: 99%

Lamin A/C proteins in the spermatid acroplaxome are essential in mouse spermiogenesis

Shen¹,

Chen²,

Shao³

et al. 2014

Reproduction

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Spermiogenesis is a complex process of terminal differentiation that is necessary to produce mature sperm. Using protein expression profiles of mouse and human testes generated from our previous studies, we chose to examine the actions of lamin A/C in the current investigation. Lamin A and lamin C are isoforms of the A-type lamins that are encoded by the LMNA gene. Our results showed that lamin A/C was expressed in the mouse testis throughout the different stages of spermatogenesis and in mature sperm. Lamin A/C was also expressed in mouse haploid germ cells and was found to be localized to the acroplaxome in spermiogenesis, from round spermatids until mature spermatozoa. The decreased expression of lamin A/C following injections of siRNA against Lmna caused a significant increase in caudal sperm head abnormalities when compared with negative controls. These abnormalities were characterized by increased fragmentation of the acrosome and abnormal vesicles, which failed to fuse to the developing acrosome. This fragmentation also caused significant alterations in nuclear elongation and acrosome formation. Furthermore, we found that lamin A/C interacted with the microtubule plus-end-tracking protein CLIP170. These results suggest that lamin A/C is critical for proper structural and functional development of the sperm acrosome and head shape.

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Section: Introductionmentioning

confidence: 99%

Lamin A/C proteins in the spermatid acroplaxome are essential in mouse spermiogenesis

Shen¹,

Chen²,

Shao³

et al. 2014

Reproduction

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“…Spermatogenesis is the process of the development of sperm from undifferentiated germ cells, via a precisely-regulated well-coordinated mechanism[16],[24]. In our previous research, we combined two-dimensional electrophoresis mass spectrometry (2DE-MS) and liquid chromatography (LC-MS) to construct a series of proteomic profiles of spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous research, we combined two-dimensional electrophoresis mass spectrometry (2DE-MS) and liquid chromatography (LC-MS) to construct a series of proteomic profiles of spermatogenesis. We also obtained a complete protein profile and proteinprotein interaction map during testicular development and spermatogenesis[16]. We selected a number of these proteins for further analysis, and in this study we investigated the localization and role of flotillin-2 during spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, the whole-protein expression profiles of different germ cells, including tetraploid and haploid germ cells, were characterized[14],[15]. These investigations have established a series of proteomic profiles associated with spermatogenesis and produced several important protein lists, which have helped to understand the regulation of male fertility and infertility[16]. Based on literature and the specificity of available antibodies, we chose several proteins from these lists for further investigation.…”

Section: Introductionmentioning

confidence: 99%

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Flotillin-2 is an acrosome-related protein involved in mouse spermiogenesis

Wu¹,

Chen²,

Wang³

et al. 2012

J Biomed Res

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Spermatogenesis is a complex process of terminal differentiation by which mature sperms are generated, and it can be divided into three phases: mitosis, meiosis and spermiogenesis. In a previous study, we established a series of proteomic profiles for spermatogenesis to understand the regulation of male fertility and infertility. Here, we further investigated the localization and the role of flotillin-2 in spermiogenesis. Flotillin-2 expression was investigated in the testis of male CD1 mice at various developmental stages of spermatogenesis by using Western blotting, immunohistochemistry and immunofluorescence. Flotillin-2 was knocked down in vivo in three-week-old male mice using intratesticular injection of small inhibitory RNA (siRNA), and sperm abnormalities were assessed three weeks later. Flotillin-2 was expressed at high levels in male germ cells during spermatogenesis. Flotillin-2 immunoreactivity was observed in pachytene spermatocytes as a strong dot-shaped signal and in round spermatids as a sickle-shaped distribution ahead of the acrosome. Immunofluorescence confirmed flotillin-2 was localized in front of the acrosome in round spermatids, indicating that flotillin-2 was localized to the Golgi apparatus. Knockdown of flotillin-2 in vivo led to a significant increase in head sperm abnormalities isolated from the cauda epididymis, compared with control siRNA-injected testes. This study indicates that flotillin-2 is a novel Golgi-related protein involved in sperm acrosome biogenesis.

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“…Examples of the advances achieved so far include, descriptive proteomics such as the identification of sperm proteins (Baker et al., ), the proteins of human seminal plasma (Pilch and Mann, 2006) and bull epididymal secretions (Belleannee et al., ). Other studies described the evolutionary and functional analysis of reproductive proteins (Findlay and Swanson, ), spermatogenesis (Huang and Sha, 2011), the explanation of changes related to chromatin condensation (Oliva and Castillo, 2011), the description of proteome during testis development (Paz et al., 2006), sperm capacitation (Baker et al., ) and the analysis of sperm regions that mediate sperm–egg interactions (Stein et al., 2006). Proteomic research has been found to be important in the diagnosis of diseases in the reproductive system, such as prostatic cancer (Masters, 2011) or asthenozoospermia (Wang et al., 2009).…”

Section: Proteomicsmentioning

confidence: 99%

The identification of seminal proteins in fish: from a traditional approach to proteomics

2012

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Summary This contribution presents an overview of the potential capabilities of evolving methodologies to characterize seminal protein profiles and identify interactions while simultaneously analyzing the complex but comprehensive protein matrix. The traditional approach to protein characterization in fish semen is based on two major steps: the purification of proteins using numerous liquid preparative chromatographic methods and the identification of proteins using sequencing. In using this approach major proteins of fish seminal plasma were identified, including apolipoprotein A‐I, transferrin, α1 – antiproteinase and parvalbumins for carp; and fetuin B‐like protein, α1 – antiproteinase, lipocalin‐type protein and apolipoprotein C‐I for rainbow trout. Proteomics provides for a great advance in protein studies. Proteomics is concerned with the study of protein products expressed by the genome, with the term ‘proteome’ originating from PROTEin complement of the genOME. Proteomics emerged as a leading technology in the post‐genomic era because of the central role of proteins and protein–protein interactions in cell physiology. The first applications of proteomic research in the study of fish reproduction have already been made, and include the effects of cryopreservation on semen proteome, molecular mechanisms determining sperm motility initiation and the effects of domestication of cultured species on semen quality. The introduction of proteomics in the study of male reproduction in fish creates a unique opportunity to unravel, using an integrative approach, the physiological mechanisms important for sperm function, such as motility and fertilizing ability. It is anticipated that proteomic studies will greatly increase the chances for reliably identifying new biomarkers in male fertility studies. However, a better understanding on the opportunities and limitations of the new disciplines or subject areas are required.

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Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

Cited by 30 publications

References 62 publications

Lamin A/C proteins in the spermatid acroplaxome are essential in mouse spermiogenesis

Lamin A/C proteins in the spermatid acroplaxome are essential in mouse spermiogenesis

Flotillin-2 is an acrosome-related protein involved in mouse spermiogenesis

The identification of seminal proteins in fish: from a traditional approach to proteomics

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