Proteomics Profiling of KAIMRC1 in Comparison to MDA-MB231 and MCF-7

Abstract

Proteomics characterization of KAIMRC1 cell line, a naturally immortalized breast cancer cells, is described in comparison to MCF-7 and MDA-MB-231 breast cancer cells. Quantitative proteomics analysis using the tandem mass tag (TMT)-labeled technique in conjunction with the phosphopeptide enrichment method was used to perform comparative profiling of proteins and phosphoproteins in the three cell lines. In total, 673 proteins and 33 Phosphoproteins were differentially expressed among these cell lines. These proteins are involved in several key cellular pathways that include DNA replication and repair, splicing machinery, amino acid metabolism, cellular energy, and estrogen signaling pathway. Many of the differentially expressed proteins are associated with different types of tumors including breast cancer. For validation, 4 highly significant expressed proteins including S-methyl-5′-thioadenosine phosphorylase (MTAP), BTB/POZ domain-containing protein (KCTD12), Poly (ADP-ribose) polymerase 1 (PARP 1), and Prelamin-A/C were subjected to western blotting, and the results were consistent with proteomics analysis. Unlike MCF-7 and MDA-MB-231, KAIMRC1 showed different phospho- and non-phosphoproteomic phenotypes which make it a potential model to study breast cancer.

Iron Oxide Mesoporous Magnetic Nanostructures with High Surface Area for Enhanced and Selective Drug Delivery to Metastatic Cancer Cells

“…While the unloaded free IO-MMNs were not toxic to any of the tested cells up to 75 µg/mL, Dox@IO-MMNs were found to be cytotoxic to both MCF-7 and KAIMRC-1 breast cancer cells (proliferation inhibition IC 50 = 3.58 µg/mL Dox, and IC 50 = 6.83 µg/mL Dox respectively) and to HT-29 colon cancer cells (IC 50 = 6.93 µg/mL Dox) ( Figure 5 ). From the IC 50 graphs, Dox@IO-MMNs showed ~2-fold enhanced cytotoxicities against the metastatic breast cancer MCF-7 in comparison to KAIMRC-1, probably due to their growth in 3D conformation [ 51 , 52 ]. The potency of Dox@IO-MMNs on MCF-7, KAIMRC-1, and HT-29 was slightly better than the potency of free Dox (IC 50 = 4.32, 7.44, and 8.09 µg/mL, respectively), which was found potent to the three different cells at almost equivalent inhibitory concentrations ( Figure 5 ).…”

Discovery of a novel potentially transforming somatic mutation in CSF2RB gene in breast cancer

“…KAIMRC1 cell line, established from a breast tumor specimen, showing growth and proliferation in the ligand starvation condition has been extensively characterized in our lab 28,29 . We hypothesized that there could be a genetic determinant conferring ligand‐independence to the KAIMRC1 cell line.…”

“…KAIMRC1 cell line, established from a breast tumor specimen, showing growth and proliferation in the ligand starvation condition has been extensively characterized in our lab. 28 , 29 We hypothesized that there could be a genetic determinant conferring ligand‐independence to the KAIMRC1 cell line. To answer this question, we designed exome sequencing of the KAIMRC1 cell line and normal PBMC cells obtained from the same breast cancer patient from which KAIMRC1 was established.…”

The Evolution of Proteomics from 2010 to 2020

“…In the same year, King Abdullah International Medical Research has published characterization of a new cell line in "protein and phosphoprotein profiling of KAIMRC1 comparison to MDA-MB-231 and MCF-7" by using mass spectrometry (MS). And this cell line can be applied for screening targets as anticancer drugs [34] .…”