Proteus mirabilis biofilm - Qualitative and quantitative colorimetric methods-based evaluation

Kwiecińska-Piróg, Joanna; Bogiel, Tomasz; Skowron, Krzysztof; Wieckowska, Ewa; Gospodarek, Eugenia

doi:10.1590/s1517-83822014000400037

Cited by 32 publications

(23 citation statements)

References 15 publications

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“…Biofilm formation by P. mirabilis has recently become an issue of increasing concern. In a previous study, P. mirabilis isolates recovered from urine samples showed a higher degree of biofilm production than those isolated from different catheter segments [28]. In this study, the prevalence of P. mirabilis was generally high, which is in agreement with studies on urine from catheterized patients.…”

Section: Discussionsupporting

confidence: 91%

“…In this study, the prevalence of P. mirabilis was generally high, which is in agreement with studies on urine from catheterized patients. [12,28]. Taken collectively, these findings indicate that further studies on P. mirabilis biofilm formation are needed to better understand the disease process and to develop new preventive and therapeutic options.…”

Section: Discussionmentioning

confidence: 89%

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Association among biofilm formation, virulence gene expression, and antibiotic resistance in Proteus mirabilis isolates from diarrhetic animals in northeast China

Sun

Wen

Zhao

et al. 2019

Preprint

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Background The aim of this study was to investigate the association among biofilm formation, virulence gene expression, and antibiotic resistance in P. mirabilis isolates collected from diarrhetic animals (n = 176) in northeast China between September 2014 and October 2016. Results Approximately 92.05% of the isolates were biofilm producers, whereas 7.95% of the isolates were non-producers. The prevalence of virulence genes in biofilm producers was significantly higher than that in non-producers. Biofilm production was significantly associated with the expression of ureC , zapA , rsmA , hmpA , mrpA , atfA , and pmfA ( P < 0.05). Drug susceptibility tests revealed that approximately 76.7% of the isolates were multidrug-resistant (MDR) and extensively drug-resistant (XDR). Biofilm production was significantly associated with resistance to doxycycline, tetracycline, sulfamethoxazole, kanamycin, and cephalothin ( P < 0.05). Although the pathogenicity of the biofilm producers was stronger than that of the non-producers, the biofilm-forming ability of the isolates was not significantly associated with morbidity and mortality in mice ( P > 0.05). Conclusion Our findings suggested that a high level of multidrug resistance in diarrhetic animals infected with P. mirabilis in northeast China.The results of this study indicated that the positive rates of the genes expressed by biofilm-producing P. mirabilis isolates were significantly higher than those expressed by non-producing isolates.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 89%

Association among biofilm formation, virulence gene expression, and antibiotic resistance in Proteus mirabilis isolates from diarrhetic animals in northeast China

Sun

Wen

Zhao

et al. 2019

Preprint

View full text Add to dashboard Cite

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“…The major advantage is that this assay allows for simultaneous screening of many samples; it is also easily quantifiable by dissolving the crystal violet and measuring the dye with a spectrophotometer. For this reason, the assay has been used to screen P. mirabilis clinical isolates and transposon mutants, as well as screening of targeted mutants (53, 177, 256, 275, 280, 281, 293, 294). Other variations on this assay include the addition of short catheter segments to multiwall plates and culture in static, closed tubes (283, 295).…”

Section: Virulence Factorsmentioning

confidence: 99%

Pathogenesis of Proteus mirabilis Infection

2018

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Proteus mirabilis, a Gram-negative rod-shaped bacterium most noted for its swarming motility and urease activity, frequently causes catheter-associated urinary tract infections (CAUTI) that are often polymicrobial. These infections may be accompanied by urolithiasis, development of bladder or kidney stones due to alkalinization of urine from urease-catalyzed urea hydrolysis. Adherence of the bacterium to epithelial and catheter surfaces is mediated by 17 different fimbriae, most notably MR/P fimbriae. Repressors of motility are often encoded by these fimbrial operons. Motility is mediated by flagella encoded on a single contiguous 54 kb chromosomal sequence. On agar plates, P. mirabilis undergoes a morphological conversion to a filamentous swarmer cell expressing hundreds of flagella. When swarms from different strains meet, a line of demarcation, a “Dienes line”, develops due to the killing action of each strain’s type VI secretion system. During infection, histological damage is caused by cytotoxins including hemolysin and a variety of proteases, some autotransported. The pathogenesis of infection, including assessment of individual genes or global screens for virulence or fitness factors has been assessed in murine models of ascending UTI or CAUTI using both single-species and polymicrobial models. Global gene expression studies carried out in culture and in the murine model have revealed the unique metabolism of this bacterium. Vaccines, using MR/P fimbria and its adhesin, MrpH, have been shown to be efficacious in the murine model. A comprehensive review of factors associated with urinary tract infection is presented, encompassing both historical perspectives and current advances.

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“…The biofilm assay was performed on 96-well polystyrene plates according to Kwiecinska-Piróg et al [21] using crystal violet. E. coli 042 [20] was used as a positive control for biofilm formation, and the TSB broth (Difco™) was used as a negative control.…”

Section: Biofilm Formationmentioning

confidence: 99%

Proteus mirabilis causing cellulitis in broiler chickens

et al. 2020

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Given the need to understand the virulence profile of Proteus mirabilis isolates from cellulitis in broiler chickens and their ability to cause lesions, the present study aimed to characterize genotypically and phenotypically the virulence profiles of two strains of P. mirabilis isolated from cellulitis in broilers, as well as to evaluate their ability to experimentally reproduce the lesions in vivo. The strain with the highest virulence potential (LBUEL-A33) possessed mrpA, pmfA, ucaA, atfA (fimbriae), zapA, ptA (proteases), hpmA (hemolysin), and ireA (siderophore) genes, formed a very strong biofilm, and expressed the pattern of aggregative adhesion and cytotoxicity in Vero cells. The strain with the lowest virulence potential (LBUEL-A34) did not present the pmfA and ucaA genes, but expressed the pattern of aggregative adhesion, formed a strong biofilm, and did not show cytotoxicity. Both strains developed cellulitis in an animal model within 24 h post-inoculation (PI), and the degree of lesions was not significantly altered up to 120 h PI. The LBUEL-A33 strain was also inoculated in combination with an avian pathogenic Escherichia coli (APEC 046), and the lesions showed no significant changes from the individual inoculation of these two strains. Histological analysis showed that the LBUEL-A33 strain developed characteristic cellulitis lesions. Thus, both strains of P. mirabilis isolated in our study have several virulence factors and the ability to develop cellulitis in broilers.

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Proteus mirabilis biofilm - Qualitative and quantitative colorimetric methods-based evaluation

Cited by 32 publications

References 15 publications

Association among biofilm formation, virulence gene expression, and antibiotic resistance in Proteus mirabilis isolates from diarrhetic animals in northeast China

Association among biofilm formation, virulence gene expression, and antibiotic resistance in Proteus mirabilis isolates from diarrhetic animals in northeast China

Pathogenesis of Proteus mirabilis Infection

Proteus mirabilis causing cellulitis in broiler chickens

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