Proteus mirabilis Employs a Contact-Dependent Killing System against Competing
            <i>Enterobacteriaceae</i>

Kiani, Dara; Santus, William; Kiernan, Kaitlyn; Behnsen, Judith

doi:10.1128/msphere.00321-21

Cited by 8 publications

(7 citation statements)

References 93 publications

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“…mirabilis and/or S. Typhimurium might have killed E. coli by, e.g., microcin secretion (ref reviewed in refs , ). Second, we saw that P.…”

Section: Results and Discussionmentioning

confidence: 99%

“…mirabilis isolates were able to kill murine E. coli isolates in coculture in the early stationary phase by using a contact-dependent killing system . The viability assay revealed that P.…”

Section: Results and Discussionmentioning

confidence: 99%

“…It was shown that different murine P. mirabilis isolates were able to kill murine E. coli isolates in coculture in the early stationary phase by using a contact-dependent killing system. 61 The viability assay revealed that P. mirabilis was able to kill E. coli (data not shown), as no living E. coli could be recovered from overnight cocultures. However, after 24 h of coculture with S. Typhimurium, the viability assay showed that a detectable but much lower number of E. coli cells were present in the overnight cultures (data not shown).…”

Section: ■ Introductionmentioning

confidence: 98%

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A Simple Biosensor-Based Assay for Quantitative Autoinducer-2 Analysis

2022

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Bacteria produce and react to interspecies signaling molecules in order to control the expression of genes that are particularly beneficial when they are expressed by a bacterial community. In addition to intraspecies communication, the signaling molecule autoinducer-2 (AI-2) can also serve for interspecies communication between Gram-positive and Gram-negative bacteria and is therefore of particular interest. The analysis and quantification of AI-2 are essential for understanding population density-dependent changes in bacterial behavior and pathogenicity. However, currently available bioassays for AI-2 quantification are rather complex, have narrow detection ranges, and are very sensitive to trace components of, for example, growth media. To facilitate and improve the detection of AI-2, we have developed an Escherichia coli biosensor-based assay that is sensitive, cheap, fast, robust, and reliable in the quantification of biologically active AI-2. The bioassay is based on an lsr promoter-fluorescent reporter gene fusion cassette that we chromosomally integrated in a biosensor strain, but the cassette can also be used in a low-copy number plasmid for the application in other Gramnegative bacterial species. We show here that AI-2 quantification was possible in a concentration range from 400 nM to 100 μM and that a critical interpretation of the kinetics of the measurements can reveal sugar interference. With the help of our biosensor strain, coculture experiments were done to test the capability and kinetics of AI-2 secretion by various Gram-negative bacteria in real time. Finally, calibration curves were used to calculate the absolute AI-2 concentration in cell-free bacterial samples.

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“…mirabilis and/or S. Typhimurium might have killed E. coli by, e.g., microcin secretion (ref reviewed in refs , ). Second, we saw that P.…”

Section: Results and Discussionmentioning

confidence: 99%

“…mirabilis isolates were able to kill murine E. coli isolates in coculture in the early stationary phase by using a contact-dependent killing system . The viability assay revealed that P.…”

Section: Results and Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 98%

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A Simple Biosensor-Based Assay for Quantitative Autoinducer-2 Analysis

2022

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“…Thanks to this, lab members can reach her anytime to update her on their newest results, like the exciting hits from a recent transposon screen. Results from this experiment will hopefully shed light on the genetic components of a new competition mechanism of Proteus mirabilis that Judith and her team described last year (Kiani et al, 2021 ). Serendipitously, they discovered the previously unknown ability of P. mirabilis , a commensal in the gut microbiota, to kill E. coli and other species of Enterobacteria .…”

Section: Being Trained and Training The Next Generation Of Scientistsmentioning

confidence: 89%

A microbiologist can't let their sourdough die

Wettstadt¹

2022

microLife

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“…In contrast, Juarez et al (2020) showed that volatile compounds produced by P. mirabilis (including ammonia) negatively affected the growth and biofilm formation of Klebsiella pneumoniae. Moreover, recent work showed that interspecies interactions between P. mirabilis and other Enterobacteriaceae may involve TVISS-independent, as yet unknown contact-dependent mechanisms (Kiani et al, 2021). Importantly, the interactions between P. mirabilis and other bacteria may also result in the acquisition of novel antibiotic resistance mechanisms through horizontal gene transfer (Bonnin et al, 2020).…”

Section: Intra-and Interspecies Interactionsmentioning

confidence: 99%

Into the understanding the multicellular lifestyle of Proteus mirabilis on solid surfaces

Gmiter

Kaca

2022

Front. Cell. Infect. Microbiol.

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Indwelling urinary catheterization can lead to the development of catheter-associated urinary tract infections (CAUTIs), an important type of nosocomial infection, as well as other medical issues among institutionalized adults. Recently, Proteus mirabilis was highlighted as the important cause of CAUTIs. The pathogenicity of P. mirabilis is dependent on two multicellular types of surface colonization: the adherence and swarming motility. Adhesion, mostly mediated by fimbrial and nonfimbrial adhesins, is important for the initiation of biofilm formation. Moreover, the production of urease frequently results in biofilm crystallization, which leads to the blockage of catheters. The heterologous polymeric matrix of the biofilm offers protection against antibiotics and the host immune system. P. mirabilis displays remarkable motility abilities. After contact with solid surfaces, hyper-flagellated cells are able to rapidly migrate. The importance of swarming motility in CAUTIs development remains controversial; however, it was indicated that swarming cells were able to co-express other virulence factors. Furthermore, flagella are strong immunomodulating proteins. On the other hand, both biofilm formation and swarming motility implicates multiple inter- and intraspecies interactions, which might contribute to the pathogenicity.

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Proteus mirabilis Employs a Contact-Dependent Killing System against Competing Enterobacteriaceae

Abstract: The study of bacterial competition systems has received significant attention in recent years. These systems are important in a multitude of polymicrobial environments and collectively shape the composition of complex ecosystems like the mammalian gut.

Cited by 8 publications

References 93 publications

A Simple Biosensor-Based Assay for Quantitative Autoinducer-2 Analysis

A Simple Biosensor-Based Assay for Quantitative Autoinducer-2 Analysis

A microbiologist can't let their sourdough die

Into the understanding the multicellular lifestyle of Proteus mirabilis on solid surfaces

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