1994
DOI: 10.1111/j.1432-1033.1994.tb18607.x
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Prothrombin activation on dioleoylphosphatidylcholine membranes

Abstract: Factor-Xa-catalyzed prothrombin activation is greatly accelerated by negatively charged phospholipids plus calcium ions. In 1990, we reported that neutral phosphatidylcholine membranes also stimulated prothrombin activation [Gerads, I., Govers-Riemslag, J. W. P., Tans, G., Zwaal, R. F. A. & Rosing, J. (1990) Biochemistry 29, 7967-79741. In the present study, we have performed a detailed analysis of the prothrombin-converting activity of phosphatidylcholine membranes. Stimulation of prothrombin activation by ph… Show more

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Cited by 19 publications
(17 citation statements)
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“…This patch represents at least part of the contact site, but electrostatic interactions involving charged amino acids may also be important (Atkins and Ganz, 1992). The interaction with unsaturated fatty acid chains of phospholipids has been described (Govers-Riemslag et al, 1994).…”
Section: Fig 11mentioning
confidence: 99%
“…This patch represents at least part of the contact site, but electrostatic interactions involving charged amino acids may also be important (Atkins and Ganz, 1992). The interaction with unsaturated fatty acid chains of phospholipids has been described (Govers-Riemslag et al, 1994).…”
Section: Fig 11mentioning
confidence: 99%
“…However, although several markers of hypercoagulability have been linked to arterial disease, it is unknown whether their effect is similar for all forms of arterial thrombosis [4][5][6][7][8][9]. There has never been a comprehensive assessment of hypercoagulability and its relation to different subtypes of arterial thrombosis.…”
Section: Introductionmentioning
confidence: 99%
“…38 First, 1 nM factor Va, 6.2 pM factor Xa, and lactadherin or annexin V at specified concentrations were incubated for 5 minutes at 25°C in a solution containing 150 mM NaCl, 50 mM Tris, 1.5 mM (for 4% PS, sonicated vesicles) or 5 mM (for the other PS content and/or vesicle size) CaCl 2 , and 0.05% wt/vol ovalbumin, pH 7.8, prior to the addition of 1 M prothrombin. After 5 minutes at 25°C, the reaction was stopped by the addition of EDTA to a final concentration of 7 mM.…”
Section: Prothrombinase Assaymentioning
confidence: 99%