Protist Diversity and Metabolic Strategy in Freshwater Lakes Are Shaped by Trophic State and Watershed Land Use on a Continental Scale

Garner, Rebecca E.; Kraemer, Susanne A.; Onana, Vera E.; Huot, Yannick; Gregory‐Eaves, Irene; Walsh, David A.

doi:10.1128/msystems.00316-22

Cited by 15 publications

(6 citation statements)

References 104 publications

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“…The trophic state of each lake was defined based on the concentration of TP based on the thresholds for Canadian freshwater systems as follows: ultraoligotrophic (TP < 4 μg/L), oligotrophic (4–10 μg/L), mesotrophic (10–20 μg/L), mesoeutrophic (20–35 μg/L), eutrophic (35–100 μg/L) and hypereutrophic (>100 μg/L; Garner et al, 2022 ). We removed 17 hyper-saline lakes from the analysis following Garner et al (2022) , leaving a dataset of 349 lake.…”

Section: Methodsmentioning

confidence: 99%

A resistome survey across hundreds of freshwater bacterial communities reveals the impacts of veterinary and human antibiotics use

et al. 2022

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Our decreasing ability to fight bacterial infections is a major health concern. It is arising due to the evolution of antimicrobial resistance (AMR) in response to the mis- and overuse of antibiotics in both human and veterinary medicine. Lakes integrate watershed processes and thus may act as receptors and reservoirs of antibiotic resistance genes (ARGs) introduced into the watershed by human activities. The resistome – the diversity of ARGs – under varying anthropogenic watershed pressures has been previously studied either focused on few select genes or few lakes. Here, we link the resistome of ~350 lakes sampled across Canada to human watershed activity, trophic status, as well as point sources of ARG pollution including wastewater treatment plants and hospitals in the watershed. A high percentage of the resistance genes detected was either unimpacted by human activity or highly prevalent in pristine lakes, highlighting the role of AMR in microbial ecology in aquatic systems, as well as a pool of genes available for potential horizontal gene transfer to pathogenic species. Nonetheless, watershed agricultural and pasture area significantly impacted the resistome. Moreover, the number of hospitals and the population density in a watershed, the volume of wastewater entering the lake, as well as the fraction of manure applied in the watershed as fertilizer significantly impacted ARG diversity. Together, these findings indicate that lake resistomes are regularly stocked with resistance genes evolved in the context of both veterinary and human antibiotics use and represent reservoirs of ARGs that require further monitoring.

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Section: Methodsmentioning

confidence: 99%

A resistome survey across hundreds of freshwater bacterial communities reveals the impacts of veterinary and human antibiotics use

et al. 2022

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“…Heterotrophic protists, which include ciliates, amoeba, perkinsozoa, cercozoan, and oomycetes, are key components of aquatic systems (Sanders, 2009). They represent a diverse and abundant fraction of planktonic assemblages in both freshwater and marine environments (Debroas et al, 2017; Garner et al, 2022; Grossmann et al, 2016) where they play various ecological roles as predators, phagotrophs, saprotrophs, parasites, and mixotrophs (Lefranc et al, 2005; Mitra et al, 2016; Oikonomou et al, 2015; Singer et al, 2021). As part of the microbial loop, they participate in the remobilisation and recycling of carbon and nutrients in aquatic ecosystems (Pernthaler & Posch, 2009).…”

Section: Beyond Oxygenic Phototrophsmentioning

confidence: 99%

Investigating the effects of anthropogenic stressors on lake biota using sedimentary DNA

et al. 2022

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Analyses of sedimentary DNA (sedDNA) have increased exponentially over the last decade and hold great potential to study the effects of anthropogenic stressors on lake biota over time. Herein, we synthesise the literature that has applied a sedDNA approach to track historical changes in lake biodiversity in response to anthropogenic impacts, with an emphasis on the past c. 200 years. We identified the following research themes that are of particular relevance: (1) eutrophication and climate change as key drivers of limnetic communities; (2) increasing homogenisation of limnetic communities across large spatial scales; and (3) the dynamics and effects of invasive species as traced in lake sediment archives. Altogether, this review highlights the potential of sedDNA to draw a more comprehensive picture of the response of lake biota to anthropogenic stressors, opening up new avenues in the field of paleoecology by unrevealing a hidden historical biodiversity, building new paleo‐indicators, and reflecting either taxonomic or functional attributes. Broadly, sedDNA analyses provide new perspectives that can inform ecosystem management, conservation, and restoration by offering an approach to measure ecological integrity and vulnerability, as well as ecosystem functioning.

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“…Our seven archetypes are comparable in number to the 11 Level I Ecoregions (Omernik 1987) in the US, which have successfully been used to group lakes with similar states or responses in a number of broad‐scale studies (Taranu et al 2017; Sprague et al 2019; Garner et al 2022). The geographic distribution of the maximum archetype weights for each lake (Fig.…”

Section: Discussionmentioning

confidence: 93%

A continuous classification of the 476,697 lakes of the conterminous US based on geographic archetypes

Lapierre,

Webster,

Hanks

et al. 2023

Limnology & Oceanography

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A variety of classification approaches are used to facilitate understanding, prediction, monitoring, and the management of lakes. However, broad‐scale applicability of current approaches is limited by either the need for in situ lake data, incompatibilities among approaches, or a lack of empirical testing of approaches based on ex situ data. We developed a new geographic classification approach for 476,697 lakes ≥ 1 ha in the conterminous U.S. based on lake archetypes representing end members along gradients of multiple geographic features. We identified seven lake archetypes with distinct combinations of climate, hydrologic, geologic, topographic, and morphometric properties. Individual lakes were assigned weights for each of the seven archetypes such that groups of lakes with similar combinations of archetype weights tended to cluster spatially (although not strictly contiguous) and to have similar limnological properties (e.g., concentrations of nutrients, chlorophyll a (Chl a), and dissolved organic carbon). Further, archetype lake classification improved commonly measured limnological relationships (e.g., between nutrients and Chl a) compared to a global model; a discrete archetype classification slightly outperformed an ecoregion classification; and considering lakes as continuous mixtures of archetypes in a more complex model further improved fit. Overall, archetype classification of US lakes as continuous mixtures of geographic features improved understanding and prediction of lake responses to limnological drivers and should help researchers and managers better characterize and forecast lake states and responses to environmental change.

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Protist Diversity and Metabolic Strategy in Freshwater Lakes Are Shaped by Trophic State and Watershed Land Use on a Continental Scale

Abstract: Freshwater lakes are experiencing rapid changes under accelerated anthropogenic stress and a warming climate. Microorganisms underpin aquatic food webs, yet little is known about how freshwater microbial communities are responding to human impact.

Cited by 15 publications

References 104 publications

A resistome survey across hundreds of freshwater bacterial communities reveals the impacts of veterinary and human antibiotics use

A resistome survey across hundreds of freshwater bacterial communities reveals the impacts of veterinary and human antibiotics use

Investigating the effects of anthropogenic stressors on lake biota using sedimentary DNA

A continuous classification of the 476,697 lakes of the conterminous US based on geographic archetypes

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