Proto-Oncogene FAM50A Can Regulate the Immune Microenvironment and Development of Hepatocellular Carcinoma In Vitro and In Vivo

Abstract

Hepatocellular carcinoma (HCC) is a vital global health problem. The characteristics are high morbidity, high mortality, difficulty in early diagnosis and insensitivity to chemotherapy. The main therapeutic schemes for treating HCC mainly include Tyrosine kinase inhibitors represented by sorafenib and lenvatinib. In recent years, immunotherapy for HCC has also achieved certain results. However, a great number of patients failed to benefit from systemic therapies. FAM50A belongs to the FAM50 family and can be used as a DNA-binding protein or transcription factor. It may take part in the splicing of RNA precursors. In studies of cancer, FAM50A has been demonstrated to participate in the progression of myeloid breast cancer and chronic lymphocytic leukemia. However, the effect of FAM50A on HCC is still unknown. In this study, we have demonstrated the cancer-promoting effects and diagnostic value of FAM50A in HCC using multiple databases and surgical samples. We identified the role of FAM50A in the tumor immune microenvironment (TIME) and immunotherapy efficacy in HCC. We also proved the effects of FAM50A on the malignancy of HCC in vitro and in vivo. In conclusion, we confirmed that FAM50A is an important proto-oncogene in HCC. FAM50A acts as a diagnostic marker, immunomodulator and therapeutic target for HCC.

Curcumin alleviates imiquimod‐induced psoriasis‐like inflammation and regulates gut microbiota of mice

“…All experiment data in this study were performed in triplicate, and statistical analyses were processed with the GraphPad Prism 7.0, which has been showed as the mean ± SD. Statistical significance of all data was examined by one‐way analysis of variance (ANOVA) or Student's t ‐test as described previously 11 . A value of p < 0.05 was considered statistically significant.…”

“…Statistical significance of all data was examined by one‐way analysis of variance (ANOVA) or Student's t ‐test as described previously. 11 A value of p < 0.05 was considered statistically significant.…”