Proto-oncogenes and p53 protein expression in normal cervical stratified squamous epithelium and cervical intra-epithelial neoplasia

Ngan, Hextan Y.S.; Liu, S.S; Yu, Hee Jeong; Liu, K.L; Cheung, Annie N.Y.

doi:10.1016/s0959-8049(99)00166-5

Cited by 11 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Little information on the involvement of c-fms and its ligand CSF-1 in cervical cancer is available. One study found no difference in the expression of c-fms in cervical precancerous lesions and normal tissue; however, c-fms levels were not examined in cervical carcinomas (21). Several studies, one by our group, showed an increase in serum and peritoneal CSF-1 levels in cervical cancer patients compared with control (3,19,20).…”

Section: Discussionmentioning

confidence: 74%

“…This study was designed to test whether cervical tumor cells express c-fms and its ligand CSF-1 as well as to examine the role of these factors in cervical carcinogenesis. One study has detected equivalent c-fms expression in precancerous cervical lesions and normal tissue but did not examine the presence of c-fms in cervical carcinomas (21). Whereas several studies have shown the expression of CSF-1 in ovarian and endometrial cancers, no other study, to our knowledge, has examined the expression of this growth factor and its receptor in malignant cervical tumors (6).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Elevated Expression of the Oncogene c-fms and Its Ligand, the Macrophage Colony-Stimulating Factor-1, in Cervical Cancer and the Role of Transforming Growth Factor-β1 in Inducing c-fms Expression

et al. 2007

View full text Add to dashboard Cite

Cervical cancer is the third most common gynecologic cancer in the United States. The presence and possible involvement of several cytokines have been studied in cervical cancer; however, very little data, if any, are available on whether cervical tumors are responsive to stimulation by the macrophage colony-stimulating factor-1 (CSF-1). Given the involvement of c-fms and its ligand CSF-1 in gynecologic cancers, such as that of the uterus and the ovaries, we have examined the expression of c-fms and CSF-1 in cervical tumor (n = 17) and normal cervix (n = 8) samples. The data show that c-fms and its ligand are significantly higher in cervical carcinomas compared with normal samples. Immunohistochemistry not only showed that tumor cells expressed significantly higher levels of c-fms but also c-fms levels were markedly higher in tumor cells than tumor-associated stromal cells. Blocking c-fms activity in cervical cancer cells, which express CSF-1 and c-fms, resulted in increased apoptosis and decreased motility compared with control, suggesting that CSF-1/c-fms signaling may be involved in enhanced survival and possibly invasion by cervical cancer cells via an autocrine mechanism. Combined, the data show for the first time the induction of CSF-1 and c-fms in cervical carcinomas and suggest that c-fms activation may play a role in cervical carcinogenesis. Additionally, our data suggest that transforming growth factor-B1 may be a factor in inducing the expression of c-fms in cervical cancer cells. The data suggest that c-fms may be a valuable therapeutic target in cervical cancer. [Cancer Res 2007;67(5):1918-26]

show abstract

Section: Discussionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Elevated Expression of the Oncogene c-fms and Its Ligand, the Macrophage Colony-Stimulating Factor-1, in Cervical Cancer and the Role of Transforming Growth Factor-β1 in Inducing c-fms Expression

et al. 2007

View full text Add to dashboard Cite

show abstract

“…EGFR was expressed in 87-93% of the superficial, intermediate and parabasal cells whereas cerbB2 was expressed in 31-52% of these cells. In contrast, EGFR was expressed in 11% of the basal cells and c-erbB2 was expressed in 81% [1]. Thus, the percentages of positive staining in normal squamous epithelium cannot be used as a reference of overexpression for cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…Binding of ligands such as EGF to the extracellular domain of the receptor initiates cellular response leading to DNA replication and cell division. Our previous study showed that EGFR was expressed in normal cervical epithelium as well as in 38-46% of the different grades of cervical intraepithelial neoplasia [1] suggesting that EGFR may play a role in cervical tumorigenesis. The findings of EGFR overexpression in cervical intraepithelial neoplasia coexisting with invasive cervical cancer further support the importance of EGFR in the progression of cervical neoplasia [2].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Abnormal Expression of Epidermal Growth Factor Receptor and c-erbB2 in Squamous Cell Carcinoma of the Cervix: Correlation with Human Papillomavirus and Prognosis

Ngan

Cheung²,

Liu³

et al. 2001

Tumor Biol

View full text Add to dashboard Cite

The aim of this study is to assess the expression of epidermal growth factor receptor (EGFR) and c-erbB2 and their correlation with human papillomavirus (HPV) status and prognosis in squamous cell carcinoma of the cervix. The expression of EGFR and c-erbB2 was studied at the protein level using the immunohistochemical (IHC) staining method, at the RNA level using the ribonuclease protection assay and at the DNA level using Southern blot and hybridization method. One hundred and one patients with squamous cell carcinoma of the cervix were recruited. Fifty-one patients were of stage 1B/2A and 50 patients were of stage 2B and above. Positive IHC stainings of EGFR and c-erbB2 proteins were found in 74.2 and 19.8% of cases, respectively. DNA amplifications of EGFR and c-erbB2 genes were detected in 35.4 and 17.2%, respectively. Of the patients showing positive EGFR and c-erbB2 staining, only 39.2 and 25%, respectively, showed DNA amplifications. RNA overexpression of EGFR or c-erbB2 was only detected in 2% of cervical cancers and was associated with positive staining and DNA amplifications. HPV was detected in 79.2% of the cases by HPV consensus primers L1, in 57.4% for HPV 16 and 27.7% for HPV 18. The abnormal expression of EGFR and c-erbB2 had no correlation with HPV detection and had no prognostic significance on survival.

show abstract

Intermediate Biomarkers

Kosmeder¹,

Pezzuto²

2001

Cancer Treatment and Research

View full text Add to dashboard Cite

Proto-oncogenes and p53 protein expression in normal cervical stratified squamous epithelium and cervical intra-epithelial neoplasia

Cited by 11 publications

References 14 publications

Elevated Expression of the Oncogene c-fms and Its Ligand, the Macrophage Colony-Stimulating Factor-1, in Cervical Cancer and the Role of Transforming Growth Factor-β1 in Inducing c-fms Expression

Elevated Expression of the Oncogene c-fms and Its Ligand, the Macrophage Colony-Stimulating Factor-1, in Cervical Cancer and the Role of Transforming Growth Factor-β1 in Inducing c-fms Expression

Abnormal Expression of Epidermal Growth Factor Receptor and c-erbB2 in Squamous Cell Carcinoma of the Cervix: Correlation with Human Papillomavirus and Prognosis

Intermediate Biomarkers

Contact Info

Product

Resources

About