Protocatechuic acid inhibits inflammatory responses in LPS-activated BV2 microglia via regulating SIRT1/NF-κB pathway contributed to the suppression of microglial activation-induced PC12 cell apoptosis

Kaewmool, Chayanut; Kongtawelert, Prachya; Phitak, Thanyaluck; Pothacharoen, Peraphan; Udomruk, Sasimol

doi:10.1016/j.jneuroim.2020.577164

Cited by 49 publications

(36 citation statements)

References 63 publications

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“…vs control group, ▲▲ p < 0.01. vs HFD group, *p < 0.05, ** p < 0.01. vs DC group, # p < 0.05, ## p < 0.01. control group (0.87 ± 0.32 vs. 4.18 ± 0.53; p < 0.01) ( Figure 2G). Compared with the HFD group, the TC, LDL and glucose levels in the DC group were significantly reduced (TC: 70.54 ± 17.76 vs. 46 Figure 2D, 2E, 2H), and the HDL levels were significantly increased (1.97 ± 0.32; p < 0.01) ( Figure 2G). There was significant difference of TG and HDL levels between the DC group and EX group (TG: 2.22 ± 0.40 vs. 1.52 ± 0.18; p < 0.05.…”

Section: Swimming Exercise and Diet Control Improved Physiological Comentioning

confidence: 91%

The effect of swimming exercise and diet on the hypothalamic inflammation of ApoE-/- mice based on SIRT1-NF-κB-GnRH expression

Wang

Yang

et al. 2020

Aging

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A high-fat diet and sedentary lifestyle could accelerate aging and hypothalamic inflammation. In order to explore the regulatory mechanisms of lifestyle in the hypothalamus, swimming exercise and diet control were applied in the high-fat diet ApoE-/-mice in our study. 20-week-old ApoE-/-mice fed with 12-week high-fat diet were treated by high-fat diet, diet control and swimming exercise. The results showed that hypothalamic inflammation, glial cells activation and cognition decline were induced by high-fat diet. Compared with the diet control, hypothalamic inflammation, glial cells activation and learning and memory impairment were effectively alleviated by swimming exercise plus diet control, which was related to the increasing expression of SIRT1, inhibiting the expression of NF-κB and raising secretion of GnRH in the hypothalamus. These findings supported the hypothesis that hypothalamic inflammation was susceptible to exercise and diet, which was strongly associated with SIRT1-NF-κB-GnRH expression in the hypothalamus.

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Section: Swimming Exercise and Diet Control Improved Physiological Comentioning

confidence: 91%

The effect of swimming exercise and diet on the hypothalamic inflammation of ApoE-/- mice based on SIRT1-NF-κB-GnRH expression

Wang

Yang

et al. 2020

Aging

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“…Many previous reports of both in vitro and in vivo studies have proved that natural polyphenols, including those from olive leaf extract, have antioxidant and anti-inflammatory properties via modulating important signalling pathways such as NF kappaB, COX-2, and iNOS [29,79]. Moreover, recently, it was shown that the upregulation of microglial SIRT1, via the phenolic compound protocatechuic acid, inhibited the release of inflammatory mediators and ameliorated microglial activation-induced neuron death [29].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, pharmacological activation or upregulation of SIRT1 may be a promising strategy for the treatment of inflammation-related neurodegenerative diseases. Till now, there are already some phytochemical compounds that have been confirmed to have the ability to increase SIRT1 expression and activity, including resveratrol, quercetin, catechin, and protocatechuic acid [29][30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Olive Leaf Polyphenols Attenuate the Clinical Course of Experimental Autoimmune Encephalomyelitis and Provide Neuroprotection by Reducing Oxidative Stress, Regulating Microglia and SIRT1, and Preserving Myelin Integrity

Giacometti

Grubić-Kezele

2020

Oxidative Medicine and Cellular Longevity

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Numerous evidences suggest that plant polyphenols may have therapeutic benefits in regulating oxidative stress and providing neuroprotection in many neurodegenerative diseases, including multiple sclerosis (MS). However, these mechanisms are not yet completely understood. In this study, we investigated the effect of olive leaf polyphenols on oxidative stress through oxidation marker level and activity (TBARS, SOD, and GPX) and their protein expression (SOD1, SOD2, and GPX1), as well as the protein expression of Sirtuin 1 (SIRT1) and microglia markers (Iba-1, CD206, and iNOS) and myelin integrity (proteolipid protein expression) in the brain of rats with induced experimental autoimmune encephalomyelitis (EAE) and subjected to olive leaf therapy. Experiments were performed in male EAE DA rats, which were randomly divided into 2 main groups: EAE groups treated with the therapy of olive leaf (EAE+TOL) and untreated EAE control groups. The EAE treated groups consumed olive leaf tea instead of drinking water (ad libitum) from the beginning to the end of the experiment. In addition, olive leaf extract was injected intraperitoneally (i.p.) for the 10 continuous days and started on the 8th day after EAE induction. The clinical course was monitored in both groups until the 30th day after EAE induction. Our results demonstrated that TOL attenuated the clinical course of EAE; reduced the oxidative stress (by decreasing the concentration of MDA); upregulated antioxidant enzymes (SOD1, SOD2, and GPX1), SIRT1 (overall and microglial), and anti-inflammatory M2 microglia; downregulated proinflammatory M1 type; and preserved myelin integrity. These data support the idea that TOL may be an effective therapeutic approach for treating MS and other neurodegenerative diseases.

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“…Since LPS induces microglia having amoeboid shape, which is characterized by the expansion of the cell body and a loss of ramifications [22], we first compared the morphology between vehicle-treated BV2 cells and LPS-treated BV2 cells. BV2 cells were exposed to vehicle or LPS for 3 days.…”

Section: Polyq-induced Morphological Changes Of Bv2 Microglial Cellsmentioning

confidence: 99%

Polyglutamine-containing microglia leads to disturbed differentiation and neurite retraction of neuron-like cells

Owada

Awata

Suzue

et al. 2020

Heliyon

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Expanded polyglutamine-containing proteins in neurons intrinsically contributes to neuronal dysfunctions and neuronal cell death in polyglutamine (polyQ) diseases. In addition, an expanded polyQ-containing protein in microglia also leads to apoptosis of neurons. However, detailed morphological analysis of neurons exposed to conditioned medium (CM) derived from polyQ-containing microglia has not been essentially carried out. Here, we introduced aggregated peptide with 69 glutamine repeat (69Q) into BV2 microglial cells. The 69Q-containing BV2 cells showed shorter branches. The CM from 69Q-containing microglia (69Q-CM) induced neurite retraction and fewer number of branch point of neurites of differentiated PC12 cells. Likewise, the 69Q-CM induces disturbed differentiation of PC12 cells with shorter total length of neurites and fewer number of branch point of neurites. Thus, the factor(s) released from polyQ-containing microglia affect both differentiation and degeneration of neuron-like cells.

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Protocatechuic acid inhibits inflammatory responses in LPS-activated BV2 microglia via regulating SIRT1/NF-κB pathway contributed to the suppression of microglial activation-induced PC12 cell apoptosis

Cited by 49 publications

References 63 publications

The effect of swimming exercise and diet on the hypothalamic inflammation of ApoE-/- mice based on SIRT1-NF-κB-GnRH expression

The effect of swimming exercise and diet on the hypothalamic inflammation of ApoE-/- mice based on SIRT1-NF-κB-GnRH expression

Olive Leaf Polyphenols Attenuate the Clinical Course of Experimental Autoimmune Encephalomyelitis and Provide Neuroprotection by Reducing Oxidative Stress, Regulating Microglia and SIRT1, and Preserving Myelin Integrity

Polyglutamine-containing microglia leads to disturbed differentiation and neurite retraction of neuron-like cells

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