Protocol for binary food choice assays using Drosophila melanogaster

Aryal, Binod; Dhakal, Subash; Shrestha, Bhanu; Sang, Jiun; Pradhan, Roshani Nhuchhen; Lee, Youngseok

doi:10.1016/j.xpro.2022.101410

Cited by 20 publications

(12 citation statements)

References 39 publications

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“…The binary choice assay was performed with minor modifications of the original protocol [ 51 , 52 ]. 1 to 3-day-old flies were collected in groups of 50 males and placed in a fresh vial for 1 day.…”

Section: Methodsmentioning

confidence: 99%

Molecular and cellular organization of odorant binding protein genes in Drosophila

Park,

Choi,

Han

et al. 2024

Heliyon

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Section: Methodsmentioning

confidence: 99%

Molecular and cellular organization of odorant binding protein genes in Drosophila

Park,

Choi,

Han

et al. 2024

Heliyon

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“…As in a prior study, binary food choice assays were performed (Aryal et al , 2022b). In a humidified chamber, 50–70 flies (3–6 days old; mixed sexes) were starved for 18 h. Next, two distinct food sources containing 1% agarose were prepared.…”

Section: Methodsmentioning

confidence: 99%

“…GR5a, GR61a, GR64b, GR64c, and GR64e are required in combination with IR25a and IR76b to detect vitamin C To address how taste receptor activities are translated into behavioral responses, we adopted two different behavioral paradigms. First, we used the well-established binary food choice assay (Aryal et al, 2022b). Vitamin C physiologically activated sweet-sensing GRNs (Fig 1G and H); therefore, no sucrose was employed in the assays.…”

Section: Gr Mutants (Fig 2b)mentioning

confidence: 99%

“…The taste of various macronutrients and micronutrients is required for the regulation of the feeding behavior of the animal (Freeman & Dahanukar, 2015; Delompré et al , 2019). Drosophila melanogaster offers an excellent choice for the researcher to perform conceivable research for the behavioral and neurophysiological analysis of taste (Moon et al , 2006; Aryal et al , 2022b). The distribution of gustatory organs throughout the body including the labellum, forelegs, pharynx, wings, and female genitalia makes the fruit fly interesting model animals (Vosshall & Stocker, 2007; Rimal & Lee, 2018; Chen & Dahanukar, 2020).…”

Section: Introductionmentioning

confidence: 99%

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The taste of vitamin C inDrosophila

2023

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Vitamins are essential micronutrients, but the mechanisms of vitamin chemoreception in animals are poorly understood. Here, we provide evidence that vitamin C doubles starvation resistance and induces egg laying in Drosophila melanogaster. Our behavioral analyses of genetically engineered and anatomically ablated flies show that fruit flies sense vitamin C via sweet-sensing gustatory receptor neurons (GRNs) in the labellum. Using a behavioral screen and in vivo electrophysiological analyses of ionotropic receptors (IRs) and sweet-sensing gustatory receptors (GRs), we find that two broadly tuned IRs (i.e., IR25a and IR76b) and five GRs (i.e., GR5a, GR61a, GR64b, GR64c, and GR64e) are essential for vitamin C detection. Thus, vitamin C is directly detected by the fly labellum and requires at least two distinct receptor types. Next, we expand our electrophysiological study to test attractive tastants such as sugars, carboxylic acids, and glycerol. Our analysis elucidates the molecular basis of chemoreception in sweet-sensing GRNs.

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“…We conducted binary food choice assays as described 61 . Briefly, two mixtures were prepared, one of which consisted of 1% agarose, the indicated concentration of NaCl and 5 mM sucrose and red food dye (sulforhodamine B, 0.1 mg/ml).…”

Section: Methodsmentioning

confidence: 99%

A single pair of pharyngeal neurons functions as a commander to reject high salt inDrosophila melanogaster

Sang,

Dhakal,

Shrestha

et al. 2023

Preprint

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Salt is a crucial for survival, while excessive NaCl can be detrimental. In the fruit fly, Drosophila melanogaster, an internal taste organ, the pharynx, is a critical gatekeeper impacting the decision to accept or reject a food. Currently, our understanding of the mechanism through which pharyngeal gustatory receptor neurons (GRNs) sense high salt are rudimentary. Here, we found that a member of the ionotropic receptor family, IR60b, is exclusively expressed in a pair of GRNs activated by high salt. Using a two-way choice assay (DrosoX) to measure ingestion, we demonstrate that IR60b and two coreceptors IR25a and IR76b, are required to prevent high salt consumption. Mutants lacking external taste organs but retaining the pharynx exhibit much higher salt avoidance than flies with all taste organs but missing the three IRs. Our findings highlight the critical role for IRs in a pair of pharyngeal GRNs to control ingestion of high salt.

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Protocol for binary food choice assays using Drosophila melanogaster

Cited by 20 publications

References 39 publications

Molecular and cellular organization of odorant binding protein genes in Drosophila

Molecular and cellular organization of odorant binding protein genes in Drosophila

The taste of vitamin C inDrosophila

A single pair of pharyngeal neurons functions as a commander to reject high salt inDrosophila melanogaster

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