Protocol for CRISPR/Cas Genome Editing for Investigating Cell Communication Network

Okusha, Yuka; Eguchi, Takanori

doi:10.1007/978-1-0716-2744-0_11

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…Articles by Eguchi et al (2023) detail the efficient purification of exosomes for investigation of CCN proteins deliverable in exosomes, and gene editing. Okusha and Eguchi (2023) provided a protocol for CRISPR/cas genome editing applicable to CCN. Kuboto (2023) generously provided the way public databases can be accessed and scrutinized for new ideas on CCN.…”

Section: Ccn6 Discussionmentioning

confidence: 99%

CCN proteins: opportunities for clinical studies—a personal perspective

Yeger

2023

J. Cell Commun. Signal.

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The diverse members of the CCN family now designated as CCN1(CYR61), CCN2 (CTGF), CCN3(NOV), CCN4(WISP1), CCN5(WISP2), CCN6(WISP3) are a conserved matricellular family of proteins exhibiting a spectrum of functional properties throughout all organs in the body. Interaction with cell membrane receptors such as integrins trigger intracellular signaling pathways. Proteolytically cleaved fragments (constituting the active domains) can be transported to the nucleus and perform transcriptional relevant functional activities. Notably, as also found in other protein families some members act opposite to others creating a system of functionally relevant checks and balances. It has become apparent that these proteins are secreted into the circulation, are quantifiable, and can serve as disease biomarkers. How they might also serve as homeostatic regulators is just becoming appreciated. In this review I have attempted to highlight the most recent evidence under the subcategories of cancer and non‐cancer relevant that could lead to potential therapeutic approaches or ideas that can be factored into clinical advances. I have added my own personal perspective on feasibility.

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Section: Ccn6 Discussionmentioning

confidence: 99%