Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID

Traumatic brain injury (TBI) activates the NF-κB pathway in microglia and astrocytes, which secrete pro-inflammatory cytokines that disrupt the blood−brain barrier (BBB). Curdlan derivatives are promising carriers for the delivery of siRNA drugs. Herein, we evaluated the glial cell specificity, siRNA delivery efficiency, and the subsequent phenotypic regulation of glial cells by the Curdlan derivatives in the TBI mouse model. Our in vitro and in vivo studies confirmed that the (1) pAVC4 or CuMAN polymer encapsulating siRNA were internalized by astrocytes and microglia in a receptor-dependent manner; (2) systemic administration of the pAVC4 or CuMAN polymer encapsulating siRNA resulted in significant gene silencing efficiency, altered the phenotypic polarization of glial cells, and regulated the secretion of inflammatory cytokines; (3) this lessened neuroinflammation, ameliorated BBB destruction, and improved vascular recovery. These data suggested that pAVC4 and CuMAN polymers are promising RNA delivery vehicles that can efficiently deliver siRNA to the target cells.

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SOLID: minimizing tissue distortion for brain-wide profiling of diverse architectures

Zhu,

Liu,

Liu

et al. 2024

Nat Commun

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Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID

Cited by 3 publications

References 14 publications

Molecular signature-based labeling techniques for vascular endothelial cells

Molecular signature-based labeling techniques for vascular endothelial cells

Curdlan-Mediated Syngeneic RNAi against NF-κB in Glial Cells Protects Cerebral Vessels in the TBI Mouse Model

SOLID: minimizing tissue distortion for brain-wide profiling of diverse architectures

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