Protocol for rating seed orchard seedlots in British Columbia: quantifying genetic gain anddiversity

Stoehr, Michael; Webber, Joe; Woods, Jack

doi:10.1093/forestry/77.4.297

Cited by 30 publications

(25 citation statements)

References 11 publications

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“…Finally, the aggregate success rate of the internal and external SMP was estimated to be equal to 15.0% in this study (5.4 + 9.6 = 15.0%). This value is higher than that reported by El-Kassaby et al (1993) for one application (8.3%) and lower than the average expectations of 25% proposed by Stoehr et al (2004) and Woods (2005), but it corresponded well with the 16% reported by Stoehr et al (2006) in a lodgepole pine seed orchard study. Bridgwater et al (1993) and El-Kassaby et al (1993) determined that the SMP success rate is greatly affected by timing, number, and method of applications (see above).…”

Section: Supplemental Mass Pollinationsupporting

confidence: 65%

Pollination dynamics in a Douglas-fir seed orchard as revealed by pedigree reconstruction

Lai¹,

Funda²,

Liewlaksaneeyanawin³

et al. 2010

Ann. For. Sci.

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Abstract• Pollination dynamics was studied in a Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seed orchard using 8 nuclear microsatellite markers and pedigree reconstruction.• The seed orchard consisted of 49 parents (clones). Cone-crop management included bloom delay and supplemental mass pollination (SMP) using 12 internal and 4 external pollen donors.• A random sample of 801 bulk seeds was genotyped for both haploid megagametophyte and corresponding diploid embryo.• Using the parental population's multilocus genotypes, full pedigree reconstruction generated all the information needed to estimate the maternal, paternal, and parental reproductive success, selfing, pollen contamination, and pollination success of the 4 external pollen donors.• Maternal, paternal, and parental reproductive success varied with 80% of gametes being produced by 23, 45, and 37% of the orchard's parents, respectively, resulting in a drastically reduced effective population size as compared to the census number (14 vs. 53).• Selfing, pollen contamination, and aggregate SMP success (internal and external) were estimated to be 15.2, 10.4, and 15.0%, respectively.• Full pedigree reconstruction was effective in unraveling the orchard's pollination dynamics and both female and male reproductive success.

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Section: Supplemental Mass Pollinationsupporting

confidence: 65%

Pollination dynamics in a Douglas-fir seed orchard as revealed by pedigree reconstruction

Lai¹,

Funda²,

Liewlaksaneeyanawin³

et al. 2010

Ann. For. Sci.

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“…Owing to the recognition of high importance of the genetic diversity of forest stands, the concept of effective number of parents was implemented in forestry jurisdictions, e.g., in Alberta and British Columbia where N e (paternal and maternal combined) of any seed lot to be used for reforestation of Crown land must not drop below 18 and 10, respectively (Anonymous 2009;Stoehr et al 2004). The latter value is derived from the presumption that N e of 10 secures capturing of 95 % of the genetic diversity existing in the base population (Nei 1973;Yanchuk 2001).…”

Section: Mating Structure In Isolation Tents and Genetic Consequencesmentioning

confidence: 99%

Mating dynamics of Scots pine in isolation tents

Funda

Wennström

Almqvist

et al. 2016

Tree Genetics & Genomes

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Seed orchards are forest tree production populations for supplying the forest industry with consistent and abundant seed crops of superior genetic quality. However, genetic quality can be severely affected by non-random mating among parents and the occurrence of background pollination. This study analyzed mating structure and background pollination in six large isolation tents established in a clonal Scots pine seed orchard in northern Sweden. The isolation tents were intended to form a physical barrier against background pollen and induce earlier flowering relative to the surrounding trees. We scored flowering phenology inside and outside the tents and tracked airborne pollen density inside and outside the seed orchard in three consecutive pollination seasons. We genotyped 5683 offspring collected from the tents and open controls using nine microsatellite loci, and assigned paternity using simple exclusion method. We found that tent trees shed pollen and exhibited maximum female receptivity approximately 1 week earlier than trees in open control. The majority of matings in tents (78.3 %) occurred at distances within two trees apart (about 5 m). Selffertilization was relatively high (average 21.8 %) in tents without supplemental pollination (SP), but it was substantially reduced in tents with SP (average 7.7 %). Pollen contamination was low in open controls (4.8-7.1 %), and all tents remained entirely free of foreign pollen. Our study demonstrates that tent isolation is effective in blocking pollen immigration and in manipulating flowering phenology. When complimented with supplemental pollination, it could become a useful seed orchard management practice to optimize the gain and diversity of seed orchard crops.

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“…The estimates of ΔG and ♂Ne based on male gametic contributions obtained from the DNA analysis (i.e., pedigree reconstruction) were compared to those obtained from visual assessment of male reproductive output (Stoehr et al, 2004). Pollen contamination (gene flow) was not considered into the male effective population size calculation.…”

Section: Assessment Of Genetic Gain and Diversitymentioning

confidence: 99%

“…The genetic quality of the resultant seed crops is of importance since seeds are the conduct for passing breeders' efforts to the forests. Parental male (pollen) and female (seed) gametic contribution, level of inbreeding, and the extent of pollen migration (contamination) from outside sources, all determine the genetic quality of these seed crops (Stoehr et al, 2004). The availability of highly informative DNA markers such as microsatellites (Vendramin and Hansen 2005) and the development of sophisticated pedigree reconstruction methods (Jones and Ardren, 2003) made it possible to reconstruct the genetic relationships among seeds/individuals derived from natural open-pollination and classify them into their respective FS and HS families.…”

Section: Introductionmentioning

confidence: 99%

Pedigree and mating system analyses in a western larch (Larix occidentalis Nutt.) experimental population

et al. 2008

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. Pedigree and mating system analyses in a western larch (Larix occidentalis Nutt.) experimental population. Annals of Forest Science, Springer Verlag/EDP Sciences, 2008, 65 (7), pp.1. Ann. For. Sci. 65 (2008) Abstract•The mating pattern and gene flow in a western larch (Larix occidentalis Nutt.) experimental population was studied with the aid of microsatellite markers and a combination of paternity-mating system analysis. The commonly difficult to assess, male gametic contribution was determined with 95% confidence and its impact on genetic gain and diversity was determined.• Male fertility success rate ranged between 0 and 11%. Male reproductive output parental imbalance was observed with 50% of the pollen being produced by the top 5% of males while the lower 39% males only produced 10% of the pollen.• A significant difference was observed between male effective population size (genetic diversity) estimates from paternity assignment compared to those based on population's census number (21 vs. 41); however, this difference did not affect estimates of genetic gain.• A total of 221 full-fib families were identified (sample size range: 1-8) and were nested among the studied 14 seed-donors.• A combination of paternity-mating system analysis is recommended to provide a better insight into seed orchards' mating dynamics. While pollen flow tends to inflate mating system's outcrossing rate, the paternity analysis effectively determined the rate and magnitude of contamination across receptive females. • Le taux de succès reproductif mâle était compris entre 0 et 11 %. Un déséquilibre dans la contribution des parents mâles a été observé avec la production de 50 % du pollen par 5 % des pères alors que 39 % d'entre eux ne contribuaient que pour seulement 10 % du pollen. Mots-clés• Une différence significative a été observée entre la taille efficace de la population mâle (diversité génétique) estimée par la recherche de paternité et celle basée sur les effectifs recensés de la population (21 vs. 41) ; cependant, cette différence n'affecte pas l'estimation du gain génétique.• 221 familles de plein-frères ont été identifiées (effectifs entre 1 et 8), regroupées parmi les 14 arbresmères étudiés.• La combinaison d'une analyse de paternité et du système de reproduction est recommandée pour étudier de manière approfondie la dynamique de croisement en vergers à graines. Tandis que les flux de pollen tendent à augmenter le taux d'inter-croisements, l'analyse de paternité détermine de manière effective le taux et l'amplitude de contamination des arbres-mères.

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Protocol for rating seed orchard seedlots in British Columbia: quantifying genetic gain anddiversity

Cited by 30 publications

References 11 publications

Pollination dynamics in a Douglas-fir seed orchard as revealed by pedigree reconstruction

Pollination dynamics in a Douglas-fir seed orchard as revealed by pedigree reconstruction

Mating dynamics of Scots pine in isolation tents

Pedigree and mating system analyses in a western larch (Larix occidentalis Nutt.) experimental population

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