Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition

Abstract

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Enhancement of cardenolide production in transgenic Digitalis purpurea L. by expressing a progesterone-5β-reductase from Arabidopsis thaliana L.

“…Therefore, the enhancement of cardenolide production might rest on the genetic engineering of Digitalis spp. (Pérez-Alonso et al, 2016) Agrobacterium rhizogenes (Riker) Conn strains were used in the initial reports of genetic transformation in the genus Digitalis (Saito et al, 1990(Saito et al, , 1991Pradel et al, 1997). But the low efficiency or inability to regenerate plants from hairy roots led investigators to make the transfer with Agrobacterium tumefaciens (Smith & Townsend) Conn. For this, wild strains and strains engineered with transformation vectors containing various transgenes were used (Lehmann et al, 1995;Sales et al, 2002Sales et al, , 2003.…”

Advancement in Plant Tissue Culture-Based Research for Sustainable Exploitation of Well-Known Medicinal Herb <i>Bacopa Monnieri</i>

“…Sterile distilled water was used to water the plants under these conditions. A survivability rate of 100% was reported for these plants under glass house conditions (Sharma et al 2016;Ali et al 2020).…”

Anti-inflammatory compounds produced in hairy roots culture of Sphaeralcea angustifolia

“…These cultures are considered an important biotechnological tool for secondary metabolites production with biological activity (Gutierrez-Valdes et al, 2020). Hairy roots have genetic and biochemical stability, can grow in media lacking plant growth regulators, and have short duplication times, attributes that give them advantages over cell suspension cultures (Rajashekar et al, 2016). Hairy root cultures of Cichorium intybus L. have been scaled up to acoustic mist bioreactor to produce esculin, a coumarin with antiin ammatory activity (Bais et al, 2002).…”