Protocols for the measurement of the F 2 -isoprostane, 15( S )-8- iso -prostaglandin F 2α , in biological samples by GC–MS or GC–MS/MS coupled with immunoaffinity column chromatography

Tsikas, Dimitrios; Suchy, Maria-Theresia

doi:10.1016/j.jchromb.2014.12.019

Cited by 20 publications

(14 citation statements)

References 25 publications

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“…Particularly, the latter allows gaining selectivity by choosing analyte specific fragments [89]. Advanced protocols for GC-MS and GC-MS/MS analysis have recently been published by Tsikas et al [90].…”

Section: Gc-ms and Gc-ms/ms Analysismentioning

confidence: 99%

Isoprostanes, neuroprostanes and phytoprostanes: An overview of 25 years of research in chemistry and biology

Galano

Lee

Oger

et al. 2017

Progress in Lipid Research

144

153

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Since the beginning of the 1990's diverse types of metabolites originating from polyunsaturated fatty acids, formed under autooxidative conditions were discovered. Known as prostaglandin isomers (or isoprostanoids) originating from arachidonic acid, neuroprostanes from docosahexaenoic acid, and phytoprostanes from α-linolenic acid proved to be prevalent in biology. The syntheses of these compounds by organic chemists and the development of sophisticated mass spectrometry methods has boosted our understanding of the isoprostanoid biology. In recent years, it has become accepted that these molecules not only serve as markers of oxidative damage but also exhibit a wide range of bioactivities. In addition, isoprostanoids have emerged as indicators of oxidative stress in humans and their environment. This review explores in detail the isoprostanoid chemistry and biology that has been achieved in the past three decades.

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Section: Gc-ms and Gc-ms/ms Analysismentioning

confidence: 99%

Isoprostanes, neuroprostanes and phytoprostanes: An overview of 25 years of research in chemistry and biology

Galano

Lee

Oger

et al. 2017

Progress in Lipid Research

144

153

View full text Add to dashboard Cite

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“…Application of supplemental iron to suckling piglets raises questions about the toxic effects of exposure to high iron levels at an early age. Considering that excess iron induces reactive oxygen species, we determined in 28-day old piglets from both experimental groups blood plasma and hepatic levels of 8-isoprostane, a biomarker providing a reliable measure of oxidative stress (Tsikas and Suchy, 2016). We found that in plasma of SFP-supplemented piglets, 8-isoprostane level were only half that of measured in FeDex-treated animals (Table 4).…”

Section: Blood Plasma and Hepatic 8-isoprostane Levels In Piglets Supmentioning

confidence: 99%

Innovative oral sucrosomial ferric pyrophosphate-based supplementation rescues suckling piglets from iron deficiency anemia similarly to commonly used parenteral therapy with iron dextran

Szudzik

Mazgaj

Lipiński

et al. 2021

Annals of Animal Science

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Iron deficiency is the most common mammalian nutritional deficiency during the neonatal period. However, among mammalian species neonatal iron deficiency anemia (IDA), the most severe consequence of iron scarcity, occurs regularly in pigs. Although intramuscular supplementation of piglets with high amounts of iron dextran (FeDex) is largely considered an appropriate preventive therapy for IDA prophylaxis, an increasing evidence shows that it negatively affects pig physiology. The aim of our study was to evaluate the efficacy of non-invasive supplementation of piglets with sucrosomial ferric pyrophosphate (SFP), a highly bioavailable dietary iron supplement in preventing IDA, in humans and mice. Results of our study show that SFP given to piglets per os in the amount of 6 mg Fe daily efficiently counteracts IDA at a rate comparable with the traditional FeDex-based supplementation (100 mgFe/kG b.w.; i.m. injection). This was indicated by physiological values of red blood cell indices and plasma iron parameters measured in 28-day old piglets. Moreover, SFP-supplemented piglets showed significantly lower (P ≤0.05) plasma level of 8-isoprostane, a biomarker for oxidative stress compared to FeDex-treated animals, implying lesser toxicity of this order of iron replenishment. Finally, supplementation with SFP does not increase considerably the blood plasma hepcidin, a peptide that acts to inhibit iron absorption from the diet. SFP emerges as a promising nutritional iron supplement, with a high potential to be adopted in the postnatal period.

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“…[ 16 – 18 ] 8-epi-prostaglandin F 2α (8-epi-PGF 2α ) is a relatively abundant member of the F2-isoprostane family and is the most representative isomer. [ 19 – 21 ] 8-epi-PGF 2α induced hepatic stellate cell (HSC) proliferation and collagen production mediated liver fibrosis. [ 22 – 24 ] In addition, a recent study showed that the effect of 8-epi-PGF 2α on HSCs is dependent on the expression of the prostaglandin-related receptor TxA 2 r. [ 23 , 25 ] Therefore, we hypothesized that 8-epi-PGF 2α might promote PSC activation mediated through TxA 2 r. The purpose of this study was to investigate the role of TxA 2 r in the activation of PSCs induced by 8-epi-PGF 2α .…”

Section: Introductionmentioning

confidence: 99%

Thromboxane A2 receptor contributes to the activation of rat pancreatic stellate cells induced by 8-epi-prostaglandin F2α

Zhang

Cui

et al. 2020

Chinese Medical Journal

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Background Pancreatic stellate cells (PSCs) activation plays a critical role in the development of chronic pancreatitis. Previous studies confirmed that thromboxane A2 receptor (TxA 2 r) was overexpressed in activated PSCs in rats. The purpose of this study was to investigate the role of TxA 2 r in the activation of PSCs induced by 8-epi-prostaglandin F 2α (8-epi-PGF 2α ). Methods TxA 2 r expression in both quiescent and activated PSCs was detected by immunocytochemistry and immunoblot assay. Isolated PSCs were treated with 8-epi-PGF 2α (10 −6 , 10 −7 , 10 −8 mol/L) for 48 h, and SQ29548 (10 −4 , 10 −6 , and 10 −7 mol/L), a TxA 2 r-specific antagonist for 48 h, respectively, to identify the drug concentration with the best biological effect and the least cytotoxicity. Then isolated PSCs were treated with SQ29548 (10 −4 mol/L) for 2 h, followed by 10 −7 mol/L 8-epi-PGF 2α for 48 h. Real-time polymerase chain reaction was performed to detect the messenger RNA (mRNA) levels of α-smooth muscle actin (α-SMA) and collagen I. Comparisons between the groups were performed using Student's t test. Results TxA 2 r was up-regulated in activated PSCs in vitro compared with quiescent PSCs (all P < 0.001). Compared with the control group, different concentrations of 8-epi-PGF 2α significantly increased mRNA levels of α-SMA (10 −6 mol/L: 2.23 ± 0.18 vs. 1.00 ± 0.07, t = 10.70, P < 0.001; 10 −7 mol/L: 2.91 ± 0.29 vs. 1.01 ± 0.08, t = 10.83, P < 0.001; 10 −8 mol/L, 1.67 ± 0.07 vs. 1.00 ± 0.08, t = 11.40, P < 0.001) and collagen I (10 −6 mol/L: 2.68 ± 0.09 vs. 1.00 ± 0.07, t = 24.94, P < 0.001; 10 −7 mol/L: 2.12 ± 0.29 vs. 1.01 ± 0.12 , t = 6.08, P < 0.001; 10 −8 mol/L: 1.46 ± 0.15 vs. 1.00 ± 0.05, t = 4.93, P = 0.008). However, different concen...

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Protocols for the measurement of the F 2 -isoprostane, 15( S )-8- iso -prostaglandin F 2α , in biological samples by GC–MS or GC–MS/MS coupled with immunoaffinity column chromatography

Cited by 20 publications

References 25 publications

Isoprostanes, neuroprostanes and phytoprostanes: An overview of 25 years of research in chemistry and biology

Isoprostanes, neuroprostanes and phytoprostanes: An overview of 25 years of research in chemistry and biology

Innovative oral sucrosomial ferric pyrophosphate-based supplementation rescues suckling piglets from iron deficiency anemia similarly to commonly used parenteral therapy with iron dextran

Thromboxane A2 receptor contributes to the activation of rat pancreatic stellate cells induced by 8-epi-prostaglandin F2α

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