Proton affinity differences among three <i>N</i>‐acetylhexosamines studied by the kinetic method

Nagaveni, V.; Vairamani, M.

doi:10.1002/rcm.1018

Cited by 9 publications

(8 citation statements)

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“…17, 485-495 (2011) Received: 9 February 2011 n Revised: 3 June 2011 n Accepted: 21 July 2011 n Publication: 13 September 2011 sugars with simple amino acids, and the differences were mainly due to their differences in proton affinity (PA). 23,24 Here we wished to investigate whether differential interactions of amino sugars are retained with a large peptide such as insulin, and the role of PA/gas-phase basicity (GB) in complex formation. 179) and amino acids were purchased from Sigma Aldrich (Steinheim, Germany).…”

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confidence: 99%

Differential Interactions of Isomeric Amino Sugars with Insulin Studied under Electrospray Ionisation Mass Spectrometry

Nagaveni

Sravani

Prabhakar

et al. 2011

Eur J Mass Spectrom (Chichester)

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Protein-ligand interactions were studied for bovine insulin-amino sugar systems under electrospray ionisation mass spectrometry conditions. The isomeric amino sugars showed differences in the relative abundance of 1:1 protein-ligand complex formation. The electrospray ionisation and tandem mass spectrometry results of the complex clearly demonstrated that the differences in the interaction of isomeric sugars with insulin are mainly due to the differences in their gas-phase basicity. The same phenomenon is replicated in the formation of complexes between insulin and other ligands, such as amino acids, as well as in the binding of the amino sugars with amyloid β 1-40 peptide.

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confidence: 99%

Differential Interactions of Isomeric Amino Sugars with Insulin Studied under Electrospray Ionisation Mass Spectrometry

Nagaveni

Sravani

Prabhakar

et al. 2011

Eur J Mass Spectrom (Chichester)

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“…Positive‐ion APCI‐QTOF mass spectra of 0.1 mM GlcNAc, ManNAc and GalNAc in methanol containing 10 mM NH 4 Ac were obtained (data not shown). Similar to hexosamines, abundant [M + H] + ions at m/z 222.096 were observed, but [M + NH 4 ] + ions were absent (The PAs of GlcNAc, ManNAc and GalNAc are 925.7, 926.1 and 927.8 kJ/mol, respectively, which are much higher than that of NH 3, i.e. 853.6 kJ/mol.)…”

Section: Resultsmentioning

confidence: 99%

“…Positive-ion APCI-QTOF mass spectra of 0. [37] which are much higher than that of NH 3, Figure 7(B) shows the relative intensities of the peaks at m/z 186 to 126 of GlcN, ManN and GalN at different collision voltages from 9 to 15 V. As the collision voltage increased from 9 to 15 V, the relative intensities of the peaks at m/z 186 to 126 from GlcNAc decreased from 3 to 0.3, while they decreased from 1.3 to 0.1 for both ManNAc and GalNAc. Thus, GlcNAc can be differentiated from ManNAc and GalNAc at lower collision voltages, e.g.…”

Section: Differentiation Of Glcnac Mannac and Galnacmentioning

confidence: 99%

Differentiation of underivatized monosaccharides by atmospheric pressure chemical ionization quadrupole time‐of‐flight mass spectrometry

Zhu

Song

Bartmess

2012

Rapid Comm Mass Spectrometry

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“…Hexosamine and N-acetyl hexosamine isomers were distinguished in the same way. [35][36][37] Using the kinetic method, Vairamani and colleagues determined, for the first time, proton affinities of three isomeric hexosamines and three isomeric N-acetylhexosamines. In collaboration with Gross et al, 38 secondary deuterium isotope effects on the proton affinities and gas-base basicities of glycine and alanine methyl esters were studied by the kinetic method.…”

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confidence: 99%

Mass Spectrometry in India

Vairamani

Prabhakar

2012

Eur J Mass Spectrom (Chichester)

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All rights reserved EUROPEAN JOURNAL OF MASS SPECTROMETRY This review highlights the research in mass spectrometry carried out all over India, with a focus on the last two decades. There has been a phenomenal increase in the use of mass spectrometers in India during the last decade following the introduction, at the beginning of the 21st century, of rugged electrospray ionization sources for the commercially available instruments, with applications such as pharmacokinetics and bio-equivalence drug studies, pesticide residue analysis and proteomics. There have been more papers published in these areas than could be fully covered in this article. This review covers mostly research in mass spectrometry carried out continuously in institutions where advanced facilities for mass spectrometry are functional. Researchers in India, with some notable exceptions, have always used and still use commercial mass spectrometers in their various areas of research, rather than develop their unique instruments. The review is divided into three parts: organic, elemental (or atomic) and biological mass spectrometry. Organic mass spectrometry Organic and bio-organic chemistry Research in organic mass spectrometry in the early years was mainly focused on the study of mass spectral fragmentation of different classes of new compounds,and was initiated in the 1960s by the late Das and his colleagues from the National Chemical Laboratory (NCL), Pune. The first double-focusing mass spectrometer (CEC-110 B) was installed at NCL for high-resolution mass measurements. The compounds studied by Das and his colleagues included aromatic amides, Benzophenanthridine derivatives, norses

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Proton affinity differences among three N‐acetylhexosamines studied by the kinetic method

Cited by 9 publications

References 19 publications

Differential Interactions of Isomeric Amino Sugars with Insulin Studied under Electrospray Ionisation Mass Spectrometry

Differential Interactions of Isomeric Amino Sugars with Insulin Studied under Electrospray Ionisation Mass Spectrometry

Differentiation of underivatized monosaccharides by atmospheric pressure chemical ionization quadrupole time‐of‐flight mass spectrometry

Mass Spectrometry in India

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