Proton and Deuteron Relaxation Study of Molecular Dynamics in Lysozyme Solutions

Abstract

A nuclear magnetic resonance spin-lattice relaxation dispersion study of the relaxation of several magnetization components in both natural and deuterated lysozyme solutions was undertaken at 20°C. Proton and deuteron resonances were employed. The two-dimensional time evolution of the magnetization and the spin-spin relaxation were analyzed. In addition, an isotopic dilution study was performed at 5 and 30.6 MHz. The results indicate that the water proton spin-lattice relaxation rate which arises from intermolecular relaxation between the water protons and the lysozyme protons represents a relatively strong relaxation mechanism. A model for the dynamics of the water molecules, consistent with the proton and deuteron dispersions as well as with the isotopic dilution results, is presented.

The role of conformational dynamics in the activity of polymer-conjugated CalB in organic solvents

“…In acetonitrile and toluene (Fig. 1d, left and middle), we observed 3 new peaks for lipases with T 2 ranging from several microseconds to tens of milliseconds like the solubilized protein 51 (we confirmed that these new peaks were not from polymers by confirming both free polymers and polymers in the conjugate response to the same linear function shown in Fig. S14, ESI †).…”

Self-Association of Lysozyme as Seen by Magnetic Relaxation Dispersion

“…We have recently used the MRD method to study the BPTI decamer 25 and the dimer and octamer of β-lactoglobulin. 50 Water 1 H MRD [51][52][53][54][55][56] data as well as water 2 H MRD 51,55,57 and 17 O MRD 57,58 data from HEWL solutions have been reported by several groups. Only one of these studies 54 dealt with selfassociation, but the MRD data did not extend above 50 MHz and were analyzed with the aid of an expression that has subsequently been shown 59 to be physically inconsistent.…”