Proton Fluxes as a Response to External Salinity in Wild Type and NaCl-Adapted <i>Nicotiana</i> Cell Lines

Watad, A.A.; Pesci, P.; Reinhold, Leonora; Lerner, H. R.

doi:10.1104/pp.81.2.454

Cited by 51 publications

(27 citation statements)

References 19 publications

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“…Both unadapted and adapted cells were grown for 3 d in fresh medium without NaCl before use in experiments. Adapted cells transferred to medium without NaCl exhibited equivalent growth when reinoculated into medium with 300 mM NaCl as cells that were maintained in medium with 300 mM NaCl (26,27).…”

Section: Materials and Methods Cell Suspensionsmentioning

confidence: 99%

“…The cells were then reseparated from the medium and subsequently preincubated in the CaSO4 solution (similar cell to volume ratio as above) for 2 h to allow recovery from shock resulting from filtration (25,26). Aliquots of the cell suspensions were then transferred to separate vessels for experimentation.…”

Section: Materials and Methods Cell Suspensionsmentioning

confidence: 99%

“…NaCl-adapted cells were isolated from unadapted cells and maintained for 4 years in medium containing 300 mm NaCl. Prior to use, the NaCl-adapted cells were transferred to medium without NaCl by inoculating the cells for 24-h periods, sequentially, into media where the NaCl concentration was reduced by 50 mM increments (26). Both unadapted and adapted cells were grown for 3 d in fresh medium without NaCl before use in experiments.…”

Section: Materials and Methods Cell Suspensionsmentioning

confidence: 99%

“…A substantial component of increased H+ pumping was vanadate-sensitive and it has been inferred that the plasma membrane H+-ATPase has a central function in the response to alterations in the osmotic or ionic environments (2,17,18,26). Intracellular sugar uptake has been linked to this enhanced H+ pumping capacity (17).…”

mentioning

confidence: 99%

“…Proton extrusion across the plasma membrane is stimulated by a reduction in the external water potential mediated by any number of osmotic solutes (17,18,26,29). A substantial component of increased H+ pumping was vanadate-sensitive and it has been inferred that the plasma membrane H+-ATPase has a central function in the response to alterations in the osmotic or ionic environments (2,17,18,26).…”

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confidence: 99%

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Enhanced Net K⁺ Uptake Capacity of NaCl-Adapted Cells

Watad

Reuveni²,

Bressan³

et al. 1991

Plant Physiol.

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Maintenance of intracellular K+ concentrations that are not growth-limiting, in an environment of high Na+, is characteristic of NaCI-adapted cells of the glycophyte, tobacco (Nicotiana tabacum/gossii). These cells exhibited a substantially greater uptake of "Rb+ (i.e. an indicator of K+) relative to unadapted cells.Potassium uptake into NaCI-adapted cells was 1.5-fold greater than unadapted cells at 0 NaCI and 3.5-fold greater when cells were exposed to 160 millimolar NaCI. The difference in net K+ uptake between unadapted and NaCI-adapted cells was due primarily to higher rates of entry rather than to reduced K+ leakage. Presumably, enhanced K+ uptake into adapted cells is a result of electrophoretic flux, and a component of uptake may be linked to vanadate-sensitive H+ extrusion.

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Section: Materials and Methods Cell Suspensionsmentioning

confidence: 99%

Section: Materials and Methods Cell Suspensionsmentioning

confidence: 99%

Section: Materials and Methods Cell Suspensionsmentioning

confidence: 99%

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confidence: 99%