Proton magnetic resonance spectroscopic creatine correlates with creatine transporter protein density in rat brain

Sartorius, Alexander; Lugenbiel, Patrick; Mahlstedt, Magdalena M.; Schloss, Patrick D.; Vollmayr, Barbara

doi:10.1016/j.jneumeth.2008.04.028

Cited by 30 publications

(19 citation statements)

References 37 publications

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“…7). The accruing change in tCr levels in response to repeated binge EtOH treatments might reflect gradually accumulating effects on the brain’s osmotic balance (as tCr has an identified role as an osmolyte, Ross and Bluml, 2001) or energy utilization (as tCr reflects the substrates available for the brain’s high-energy phosphate metabolism, Sartorius et al, 2008). …”

Section: Discussionmentioning

confidence: 99%

Transient CNS responses to repeated binge ethanol treatment

et al. 2015

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Adaptive changes occur in response to repeated exposure to drugs. Although ethanol (EtOH) is known to induce pharmacokinetic tolerance, the effects of EtOH on in vivo, magnetic resonance (MR)-detectable brain measures across repeated exposures have not previously been reported. Of 28 rats weighing 341±22g at baseline, 15 were assigned to the EtOH group and 13 to the control (Ctrl) group. EtOH animals were exposed to 5 cycles of 4-days of EtOH treatment followed by 10 days of recovery. Rats in both groups had structural MR imaging (MRI) scans and whole brain MR spectroscopy (MRS) at baseline, immediately following each binge period, and after each recovery period (total=11 MR scans per rat). Average blood alcohol levels (BALs) across each of the 5, 4-day binge periods were 298, 300, 301, 312, 318 mg/dL. Cerebrospinal fluid (CSF) volumes of the lateral ventricles and cisterns showed enlargement with each binge EtOH exposure but recovery with each abstinence period. Similarly, changes to MRS metabolites were transient: levels of N-acetyl aspartate (NAA) and total creatine (tCr) decreased, while those of choline-containing compounds (Cho) and glutamate/glutamine (Glx) increased with each binge EtOH exposure cycle, but also recovered during each abstinence period. The directionality of changes in response to EtOH were in expected directions based on previous, single-binge EtOH exposure experiments, but the current results do not provide support for accruing pathology with repeated binge EtOH exposure.

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Section: Discussionmentioning

confidence: 99%

Transient CNS responses to repeated binge ethanol treatment

et al. 2015

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“…For example, the MR signal of creatine is often used as a reference in 1 H-labelled spectroscopy. However, this technique is only valid and useful if the concentration of one metabolite is assumed to be constant [51,52]. …”

Section: Translational Mrsmentioning

confidence: 99%

“…Creatine is partly ingested through food and partly synthetized in the liver, kidneys and pancreas from arginine, glycine and 5-adenosylmethionine. Phosphocreatine is the storage form of chemical energy in muscle, brain and nerve tissue [52]. Resonances of creatine and phosphocreatine are indistinguishable in in vivo 1 H spectra.…”

Section: Translational Mrsmentioning

confidence: 99%

Advantages and Challenges of Small Animal Magnetic Resonance Imaging as a Translational Tool

Hoyer¹,

Gass

Weber‐Fahr

et al. 2014

Neuropsychobiology

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The utilization of magnetic resonance imaging (MRI) methods in rodent models of psychiatric disorders provides considerable benefits for the identification of disease-associated brain circuits and metabolic changes. In this review, we discuss advantages and challenges of animal MRI and provide an overview of the major structural (voxel-based morphometry and diffusion tensor imaging) and functional approaches [resting-state functional MRI (rs-fMRI), MR spectroscopy (MRS), regional cerebral blood volume measurement and arterial spin labelling] that are applied in animal MRI research. The review mainly focuses on rs-fMRI and MRS. Finally, we take a look at some recent developments and refinements in the field.

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“…Western blot analysis using an affinity purified rabbit polyclonal antibody against CRT (C7911-05, Biomol GmbH, Hamburg, Germany), an affinity purified goat polyclonal antibody against b-actin (sc-1615, Santa-Cruz Biotechnology Inc, Heidelberg, Germany) and secondary antibodies goat anti-Rabbit-IgG polyklonal HRP-conjugate (sc-2004, Santa-Cruz Biotechnology) and donkey anti-Goat-IgG polyklonal HRP-conjugate (sc-2033, Santa-Cruz Biotechnology) was performed as described recently (Sartorius et al 2008). To quantify the optical density of the protein bands of CRT (50 and 70 kDa) (Speer et al 2004;Sartorius et al 2008) and b-actin (50 kDa) we used the ONE-Dscan 2.03 program (Scanalytics, Inc.).…”

Section: Methodsmentioning

confidence: 99%

“…To quantify the optical density of the protein bands of CRT (50 and 70 kDa) (Speer et al 2004;Sartorius et al 2008) and b-actin (50 kDa) we used the ONE-Dscan 2.03 program (Scanalytics, Inc.). For final statistic analysis we calculated the ratio of the sum of the optical density of both CRT bands over the nonaffected b-actin band (Vaidya et al 2000).…”

Section: Methodsmentioning

confidence: 99%

Creatine transporter expression after antidepressant therapy in rats bred for learned helplessness

Lugenbiel

Sartorius

Vollmayr

et al. 2010

The World Journal of Biological Psychiatry

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In cells and tissues with a high demand of energy such as neurons the major pool of energy is supplied by phosphocreatine. The necessary supply with creatine is directly related to the expression level of the creatine transporter (CRT). To study possible interactions between this transporter and depressive like behavior we have used a congenitally learned helplessness (cLH) model. Because uptake via CRT is the only means to supply neurons with creatine, we hypothesized a rise in CRT expression following antidepressive treatment strategies. The hippocampus of the cLH animals exhibited a significantly lower CRT expression as compared to wild type (WT) animals. Treatment with escitalopram and ECS induced an elevation of CRT levels in the hippocampus and the prefrontal cortex. In WT animals there was no treatment effect. In summary, our data presented here suggest an association between depressive behavior and cellular energy metabolism in defined brain areas.

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Proton magnetic resonance spectroscopic creatine correlates with creatine transporter protein density in rat brain

Cited by 30 publications

References 37 publications

Transient CNS responses to repeated binge ethanol treatment

Transient CNS responses to repeated binge ethanol treatment

Advantages and Challenges of Small Animal Magnetic Resonance Imaging as a Translational Tool

Creatine transporter expression after antidepressant therapy in rats bred for learned helplessness

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