Proton Magnetic Resonance Spectroscopy of Cerebrospinal Fluid as an Aid in Neurological Diagnosis.

Abstract

The proton magnetic resonance (1H-NMR) spectra were obtained from 38 cerebrospinal fluid (CSF) samples from 34 patients with various central nervous system (CNS) diseases and the H2O signals were reduced by the presaturation method. A doublet signal for CH3 protons of lactate, as well as a singlet one for alpha-CH proton of glucose, were clearly detected in all the spectra obtained. The relative lactate concentration values were calculated on the basis of the glucose concentrations which had been measured by a routine laboratory method in the hospital and the ratios of the peak heights (lactate CH3/glucose alpha-CH). In the CSF samples examined, the relative lactate values semi-quantitatively determined in this way were clearly elevated in cerebral infarction and bacterial meningitis, but not in other disorders including viral meningitis. On the other hand, one CSF component whose enhancement was found only in a sample from a hepatic encephalitis patient was identified as glutamine from the double quantum filtered-shift correlation spectrum. These results suggest that 1H-NMR spectroscopy of CSF can become a powerful aid in biochemical diagnosis of CNS diseases.

Analysis of Cerebrospinal Fluid in Diagnosis of Bacterial Meningitis; Using Nuclear Magnetic Resonance Spectroscopy: A Systematic Review

“…Matthews et al assessed the methabolic reason of encephalopathy associated with acute bacterial meningitis using Phosphorus magnetic resonance spectroscopy in conjunction with characterizing the changes of magnetic resonance spectroscopy signals during bacterial meningitis (24). The results of Phosphorus MR spectroscopy indicated the extracellular acidosis that were compatible with results of other studied which are reported in this review (19,23,14). (24).…”

“…Also in bacterial meningitis during therapy it decreases accompanied by favorable changes in the clinical response to treatment, as demonstrated by a reduction in the levels of CSF protein and CSF cell count (14).…”

Proton MR CSF analysis and a new software as predictors for the differentiation of meningitis in children

“…28,33,34,61 It has recently been observed that the analysis of CSF based on CSF cytology, sugar and protein content is not the primary information to serve for the purpose of differential diagnosis. 25 As noted during the PROTON MR CSF ANALYSIS 221 course of our present investigation, in certain cases, clinical symptoms (as observed by the clinician) were difficult to interpret and it was found that the children were unable to express themselves.…”

“…28 In recent times, high-resolution proton MR spectroscopy has beyond doubt become one of the most important analytical tools for obtaining metabolic information on biological fluid or tissue extracts. [28][29][30][31][32][33][34][35][36][37][38][39] It has been well documented that NMR spectroscopy gives non-invasive, qualitative and quantitative information regarding the metabolic profile [40][41][42][43][44][45][46][47][48][49][50][51][52] and usually a metabolite concentration of greater than a few mM is detectable in 1 H NMR spectra 53 in a reasonable amount of time ( 1 H NMR; 128 scans; approximately 15 min), and with the recent technological advances in NMR probe design such as microprobes and cryoprobes, the detection range and time taken have been reduced further using high-field magnets, 600 MHz or higher. The potential advantages of NMR and the persistent problems regarding the timely diagnosis of childhood meningitis have prompted us to explore 1 H NMR spectroscopy as a diagnostic utility based on the analysis of CSF from 191 cases.…”

Near-Infrared Spectroscopy-Derived Tissue Oxygen Saturation in Battlefield Injuries: A Case Series Report

“…Such metabolomic methods can detect genotypic and phenotypic differences even in closely related microorganisms and also in genetically modified strains [13][14][15][16][17], with greater discriminatory power than transcriptomics and proteomics [17,18]. Spectroscopic techniques characterise rapidly and simultaneously multiple chemical compounds in biological fluids [10,[19][20][21][22][23][24][25][26][27][28]. In addition, NMR spectroscopy detects low molecular weight metabolites, requires no time-consuming sample preparation and is non-destructive.…”