Proton nuclear magnetic resonance spectroscopy of urine samples in preterm asphyctic newborn: A metabolomic approach

Longini, Mariangela; Giglio, Sabrina; Perrone, Serafina; Vivi, Antonio; Tassini, Maria; Fanos, Vassilios; Sarafidis, Kosmas; Buonocore, Giuseppe

doi:10.1016/j.cca.2015.02.004

Cited by 28 publications

(29 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For measurements, the tandem mass spectrometer has been run in multiple reaction monitoring (MRM) with the electrospray source operating in negative ion mode and by exploiting the transition m/z 353.3 > 193.2 for F 2 -isoprostanes and 357.3 > 197.2 for the isotopically-labeled form used as internal standard (d4–8-iso PGF 2 α , Cayman Chemical Co., Ann Arbor, MI, USA) [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

Predictive Role of F₂-Isoprostanes as Biomarkers for Brain Damage after Neonatal Surgery

et al. 2017

Self Cite

View full text Add to dashboard Cite

Objective Neonates have a high risk of oxidative stress during anesthetic procedures. The predictive role of oxidative stress biomarkers on the occurrence of brain injury in the perioperative period has not been reported before. Methods A prospective cohort study of patients requiring major surgery in the neonatal period was conducted. Biomarker levels of nonprotein-bound iron (NPBI) in plasma and F2-isoprostane in plasma and urine before and after surgical intervention were determined. Brain injury was assessed using postoperative MRI. Results In total, 61 neonates were included, median gestational age at 39 weeks (range 31–42) and weight at 3000 grams (1400–4400). Mild to moderate brain lesions were found in 66%. Logistic regression analysis showed a significant difference between plasma NPBI in patients with nonparenchymal injury versus no brain injury: 1.34 umol/L was identified as correlation threshold for nonparenchymal injury (sensitivity 67%, specificity 91%). In the multivariable analysis, correcting for GA, no other significant relation was found with the oxidative stress biomarkers and risk factors. Conclusion Oxidative stress seems to occur during anaesthesia in this cohort of neonates. Plasma nonprotein-bound iron showed to be associated with nonparenchymal injury after surgery, with values of 1.34 umol/L or higher. Risk factors should be elucidated in a more homogeneous patient group.

show abstract

Section: Methodsmentioning

confidence: 99%

Predictive Role of F₂-Isoprostanes as Biomarkers for Brain Damage after Neonatal Surgery

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Lactate Plasma, urine, cord blood, CSF, brain tissue, ocular tissues [25,26,29,30,32,33,35,[38][39][40][41][42][45][46][47]49,51] Succinate Plasma, urine, cord blood, brain tissue [29,30,37,38,[40][41][42][43]45,47,49,50] Alanine Plasma, urine, cord blood, CSF, brain tissue [25,26,29,30,[37][38][39][40]48,49,52] Urine [36,38,45] BCAA, Branched Chain Amino Acids.…”

Section: Metabolite Sample Referencementioning

confidence: 99%

“…Almost 10 years later, Longini et al identified a panel of altered 1 H NMR urinary metabolites in 6 asphyctic preterm newborns compared to 8 matched controls [45]. They collected samples in a non-specified moment during the first 2 days of life.…”

Section: Human Studiesmentioning

confidence: 99%

Exploring Perinatal Asphyxia by Metabolomics

et al. 2020

View full text Add to dashboard Cite

Brain damage related to perinatal asphyxia is the second cause of neuro-disability worldwide. Its incidence was estimated in 2010 as 8.5 cases per 1000 live births worldwide, with no further recent improvement even in more industrialized countries. If so, hypoxic-ischemic encephalopathy is still an issue of global health concern. It is thought that a consistent number of cases may be avoided, and its sequelae may be preventable by a prompt and efficient physical and therapeutic treatment. The lack of early, reliable, and specific biomarkers has up to now hampered a more effective use of hypothermia, which represents the only validated therapy for this condition. The urge to unravel the biological modifications underlying perinatal asphyxia and hypoxic-ischemic encephalopathy needs new diagnostic and therapeutic tools. Metabolomics for its own features is a powerful approach that may help for the identification of specific metabolic profiles related to the pathological mechanism and foreseeable outcome. The metabolomic profiles of animal and human infants exposed to perinatal asphyxia or developing hypoxic-ischemic encephalopathy have so far been investigated by means of 1 H nuclear magnetic resonance spectroscopy and mass spectrometry coupled with gas or liquid chromatography, leading to the identification of promising metabolomic signatures. In this work, an extensive review of the relevant literature was performed.trigger several changes at molecular and cellular levels, which may end in cell death and in local/systemic inflammation. The shortage of oxygen, which acts as final electron acceptor in the electron transport chain (ETC) during aerobic respiration, induced by hypoxia and by ischemia, boosts reactive oxygen species (ROS) generation at the cellular level. Generated ROS attack surrounds components at both the mitochondrial and cellular level, leading to mitochondrial dysfunction and permanent damage to cells. The pathogenesis of HIE is strongly influenced by the failure of several potent fetal compensatory mechanisms to cope with the 'physiological' hypoxia during pregnancy and delivery. The final clinical outcome of such an insult is a wide spectrum of neurological deficits, ranging from behavioral and motor impairments to general developmental delays to seizures related to structural brain damage.The severity of the clinical picture of HIE infants is the final result of an uneven combination of several factors, and among them the length and strength of hypoxic insult, together with fetal metabolic conditions before the hypoxia onset. For this reason, the pathological effects are complex to forecast, and they evolve over time. They may be related to two main pathological phases: A primary and a secondary energy failure. Primary energy failure is the first biological effect of both hypoxia and a reduction of cerebral blood flow and it mainly takes place before birth. While the impairment of blood flow is responsible for the progressive reduction of glucose availability needed to fuel brain cells' metabo...

show abstract

“…Emerging studies on animal model have highlighted the importance to recognize the metabolic fingerprint in perinatal anoxia-hypoxia (19)(20)(21). In newborns with perinatal asphyxia, a small number of metabolomics studies have been published in the literature (22)(23)(24)(25)(26)(27); each of them has depicted the metabolic snapshot of the disease. As recapitulated in a recent review (28), human metabolomics studies have found that the most impaired molecular pathway during perinatal asphyxia and TH are the TCA cycle and the osmoregulation system.…”

Section: Introductionmentioning

confidence: 99%

Urinary gas chromatography mass spectrometry metabolomics in asphyxiated newborns undergoing hypothermia: from the birth to the first month of life

Noto¹,

Pomero²,

Mussap³

et al. 2016

Ann. Transl. Med.

View full text Add to dashboard Cite

Background: Perinatal asphyxia is a severe clinical condition affecting around four million newborns worldwide.It consists of an impaired gas exchange leading to three biochemical components: hypoxemia, hypercapnia and metabolic acidosis. Methods:The aim of this longitudinal experimental study was to identify the urine metabolome of newborns with perinatal asphyxia and to follow changes in urine metabolic profile over time. Twelve babies with perinatal asphyxia were included in this study; three babies died on the eighth day of life. Total-body cooling for 72 hours was carried out in all the newborns. Urine samples were collected in each baby at birth, after 48 hours during hypothermia, after the end of the therapeutic treatment (72 hours), after 1 week of life, and finally after 1 month of life. Urine metabolome at birth was considered the reference against which to compare metabolic profiles in subsequent samples. Quantitative metabolic profiling in urine samples was measured by gas chromatography mass spectrometry (GC-MS). The statistical approach was conducted by using the multivariate analysis by means of principal component analysis (PCA) and orthogonal partial least square discriminant analysis (OPLS-DA). Pathway analysis was also performed. Results:The most important metabolites depicting each time collection point were identified and compared each other. At birth before starting therapeutic hypothermia (TH), urine metabolic profiles of the three babies died after 7 days of life were closely comparable each other and significantly different from those in survivors. Conclusions:In conclusion, a plethora of data have been extracted by comparing the urine metabolome at birth with those observed at each time point collection. The modifications over time in metabolites composition and concentration, mainly originated from the depletion of cellular energy and homeostasis, seems to constitute a fingerprint of perinatal asphyxia.

show abstract

Proton nuclear magnetic resonance spectroscopy of urine samples in preterm asphyctic newborn: A metabolomic approach

Cited by 28 publications

References 36 publications

Predictive Role of F₂-Isoprostanes as Biomarkers for Brain Damage after Neonatal Surgery

Predictive Role of F₂-Isoprostanes as Biomarkers for Brain Damage after Neonatal Surgery

Exploring Perinatal Asphyxia by Metabolomics

Urinary gas chromatography mass spectrometry metabolomics in asphyxiated newborns undergoing hypothermia: from the birth to the first month of life

Contact Info

Product

Resources

About

Proton nuclear magnetic resonance spectroscopy of urine samples in preterm asphyctic newborn: A metabolomic approach

Cited by 28 publications

References 36 publications

Predictive Role of F2-Isoprostanes as Biomarkers for Brain Damage after Neonatal Surgery

Predictive Role of F2-Isoprostanes as Biomarkers for Brain Damage after Neonatal Surgery

Exploring Perinatal Asphyxia by Metabolomics

Urinary gas chromatography mass spectrometry metabolomics in asphyxiated newborns undergoing hypothermia: from the birth to the first month of life

Contact Info

Product

Resources

About

Predictive Role of F₂-Isoprostanes as Biomarkers for Brain Damage after Neonatal Surgery

Predictive Role of F₂-Isoprostanes as Biomarkers for Brain Damage after Neonatal Surgery