Proton transfer mass spectrometry of peptides. Rapid heating technique for underivatized peptides containing arginine

Beuhler, R. J.; Flanigan, Everett; Greene, Lewis Joel; Friedman, L.

doi:10.1021/ja00819a043

Cited by 194 publications

(100 citation statements)

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“…Other major ions also re ect the fundamental backbone structure of the polymer. 3) Minor secondary thermal decomposition observed with lament temperature even above 500°C is the useful gure of merit for the present technique, verifying the concept given by Beuhler et al 2) It should be noted that the less-volatility compounds dealt with desorbed at rather low lament temperatures (e.g., 154°C). 3) is suggests that there might be some tribological e ect in addition to the thermal e ect in the desorption process of less-volatility compounds.…”

Section: Flash Surface Heating/rapid Coolingsupporting

confidence: 85%

“…2) ey found that thermal desorption becomes more favorable than thermal decomposition at higher temperature for some peptides even when the activation energy of desorption is larger than the bond dissociation energy. is phenomenon was interpreted based on the "classic" Arrhenius reaction rate theory for thermal desorption and decomposition.…”

Section: Thermal Vs Non-thermal Desorptionmentioning

confidence: 99%

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Desorption Mass Spectrometry

Lyon¹

1985

ACS Symposium Series

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In mass spectrometry, analytes must be released in the gas phase. ere are two representative methods for the gasi cation of the condensed samples, i.e., ablation and desorption. While ablation is based on the explosion induced by the energy accumulated in the condensed matrix, desorption is a single molecular process taking place on the surface. In this paper, desorption methods for mass spectrometry developed in our laboratory: ash heating/rapid cooling, Leidenfrost phenomenon-assisted thermal desorption (LPTD), solid/solid friction, liquid/solid friction, electrospray droplet impact (EDI) ionization/desorption, and probe electrospray ionization (PESI), will be described. All the methods are concerned with the surface and interface phenomena. e concept of how to desorb less-volatility compounds from the surface will be discussed.Copyright © 2017 Dilshadbek Tursunbayevich Usmanov, Satoshi Ninomiya, Lee Chuin Chen, Subhrakanti Saha, Mridul Kanti Mandal, Yuji Sakai, Rio Takaishi, Ahsan Habib, Kenzo Hiraoka, Kentaro Yoshimura, Sen Takeda, Hiroshi Wada, and Hiroshi Nonami. is is an open access article distributed under the terms of Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.Please cite this article as: Mass Spectrom (Tokyo) 2017; 6(2): S0059 Keywords: desorption, ablation, thermal desorption, Leidenfrost phenomenon, ash heating, electrospray droplet impact ionization, probe electrospray ionization (Received September 20, 2016; Accepted January 4, 2017) RATIONALE: ABLATION VS. DESORPTION e two technical terms, ablation and desorption, should be distinguished rigorously. As shown in Fig. 1, ablation is induced by explosion of the matrix or substrate whereas desorption is a single molecular event, i.e., detachment of a single molecule from the surface. e antonym of desorption is adsorption.In matrix assisted laser desorption/ionization (MALDI), for example, the photon energy is ultimately converted to thermal energy and ablation of the matrix takes place above the critical laser power (Fig. 1(a)). In ablation, the ablated materials are mainly composed of aggregates. us the ions observed in MALDI are only a small part of materials ablated. In this respect, MALDI is more likely matrixassisted laser "ablation" ionization. In secondary ion mass spectrometry (SIMS), primary projectiles such as Ar + , Cs + , O 2 + , Bi 3 + , C + 60 , and (Ar) n + are used. In SIMS using atomic ion projectiles, the sputtering is caused by the cascade collisions in the sample. us the useful yield de ned as [total gas phase ions detected] divided by [total amount of sample sputtered] is rather low. e small useful yields for MALDI and SIMS (10 −5 -10 −6 ) originate from the fact that photons or primary beams penetrate into the target materials leading to the sputtering of the substrate. In contrast, the useful yield for desorption-based techniques could be much higher than MALDI and SIMS if the desor...

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Section: Flash Surface Heating/rapid Coolingsupporting

confidence: 85%

Section: Thermal Vs Non-thermal Desorptionmentioning

confidence: 99%

Desorption Mass Spectrometry

Lyon¹

1985

ACS Symposium Series

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“…Also, this low absorptivity means that when dealing with a relatively thick layer of organic material, the temperature distribution at the surface and in the bulk will be important. The absorption coefficient, therefore, affects the rate of Early studies on the evaporation of thermolabile molecules from rapidly heated wires suggested that normally thermolabile molecules could be desorbed if heated rapidly enough [31]. This effect could be explained in terms of the activation energies of the evaporation and pyrolysis reactions.…”

Section: Discussionmentioning

confidence: 99%

Infrared Desorption of Neutral Molecules Embedded in Transparent Matrices

Beavis

Lindner

Grotemeyer

et al. 1988

Zeitschrift Für Naturforschung A

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I n f r a r e d D e s o r p tio n o f N e u t r a l M o le c u le s E m b e d d e d in T r a n s p a r e n t M a tr ic e s R.C. Beavis, J. Lindner, J. Grotemeyer, and E.W. Schlag Institut für Physikalische und Theoretische Chemie, Technische Universität München Z. Naturforsch. 43a, 1083Naturforsch. 43a, -1090Naturforsch. 43a, (1988; received July 25, 1988 The desorption of intact molecules by pulsed IR irradiation is shown to depend on the IR absorption of the matrix. IR transparent matrices, such as NaCl or NH4N03, increase the total yield and the shot-to-shot stability of the sample. IR absorbing matrices decrease the yield of intact molecules and increase the amount of pyrolysis products.Using semi-transparent sugar matrices with dipeptide samples, the most intense fragment, i.e. the parent molecule having lost 18 u, is suppressed. This fragment is formed by pyrolysis during IR desorption. Three other prominent fragments are caused by the UV photoionization step.The experimental results suggest that a model involving bulk stresses and strain may be necessary to explain the observations.

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“…In the thermal mechanism, the surface nanostructures provide a thermal conduction barrier that tends to trap absorbed laser energy momentarily in the nano-dimension. It thus causes a rapid surface heating, which may promote intact desorption of thermally labile molecules [12,13]. On the other hand, nonthermal contributions to the desorption process have also been proposed.…”

Section: Introductionmentioning

confidence: 99%

Laser-Induced Thermal Desorption Facilitates Postsource Decay of Peptide Ions

Kim

Lee

Song

et al. 2012

J. Am. Soc. Mass Spectrom.

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We investigated the thermal mechanism involved in laser desorption/ionization (LDI) of thermally labile molecules from the flat surfaces of amorphous Si (a-Si) and crystalline Si (c-Si). a-Si was selected for this study because of its thermal property, such as low thermal conductivity; thus, it was predicted to be highly susceptible to laser-induced surface heating. By virtue of lack of surface nanostructures, the flat surfaces offer a simple model system to focus on the thermal mechanism, avoiding other effects, including possible non-thermal contributions that can arise from the physical existence of surface nanostructures. For the energetics study, the internal energies of substituted benzylpyridinium ions produced by LDI on the bare and coated surfaces of a-Si and c-Si were obtained using the survival yield method. The results, including LDI thresholds, ion yields, and internal energies all suggested that the LDI mechanism would be indeed thermal, which is most likely promoted by thermal desorption caused by laser-induced surface heating. In addition, the LDI process driven by laser-induced thermal desorption (LITD) was also found to be capable of depositing an excessive internal energy in resulting LDI ions, which underwent a dissociation. It exhibited the essentially same features as in postsource decay (PSD) in MALDI-TOF/TOF mass spectrometry. We report that the LDI process by LITD offers not only a way of intact ionization but also a facile means for PSD of peptide ions, which this work demonstrates is well suited to peptide sequencing using TOF/TOF mass spectrometry.

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Proton transfer mass spectrometry of peptides. Rapid heating technique for underivatized peptides containing arginine

Abstract: Consideration of the kinetics of the evaporation of peptides leads to two approaches to volatility enhancement. An obvious direction to take is to attempt to reduce the energy of bonding of molecules to surfaces by dispersal onto surfaces which are relatively inert.

Cited by 194 publications

References 1 publication

Desorption Mass Spectrometry

Desorption Mass Spectrometry

Infrared Desorption of Neutral Molecules Embedded in Transparent Matrices

Laser-Induced Thermal Desorption Facilitates Postsource Decay of Peptide Ions

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