Protonation-induced pH increase at the triblock copolymer micelle interface for transient membrane permeability at neutral pH

Sarkar, Yeasmin; Roy, Snigdha; Majumder, Rini; Das, Sanju; Bhalani, Dixit V.; Ray, Ambarish; Jewrajka, Suresh K.; Parui, Partha Pratim

doi:10.1039/c9sm01002e

Cited by 3 publications

(3 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For this, thin lipid films are obtained from organic solutions containing all lipid components. The films are rehydrated with an aqueous calcein buffer, followed by multiple freeze-thaw cycles, [102,105,108,110] and repeated extrusion through polycarbonate membranes with pore sizes of 100 nm, [101,102,[104][105][106]108,109,113] or larger. [110,115] The outer buffer with free dye is exchanged via size exclusion chromatography (SEC) or centrifugation protocols.…”

Section: Calcein Leakage From Artificial Model Vesiclesmentioning

confidence: 99%

“…[104][105][106]109] By contrast, Sarkar et al used HEPES buffer (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) to investigate calcein leakage of triblock copolymer micelles by varying the pH range from 5.8 to 7.6 outside the liposomes. [102] Furthermore, glucose was added to balance the different osmotic pressures inside and outside the liposomes, whereas other studies mostly used NaCl or KCl for compensation. If very high calcein concentrations (>70 mm) are required, also the weak solubility of calcein at low pH values has to be considered which requires the use of basic buffers (e.g., Tris-HCl pH 10) [103] for the hydration of the lipid film.…”

Section: Calcein Leakage From Artificial Model Vesiclesmentioning

confidence: 99%

See 1 more Smart Citation

Tracking the Endosomal Escape: A Closer Look at Calcein and Related Reporters

Hausig‐Punke

Richter

Hoernke

et al. 2022

Macromolecular Bioscience

View full text Add to dashboard Cite

Crossing the cellular membrane and delivering active pharmaceuticals or biologicals into the cytosol of cells is an essential step in the development of nanomedicines. One of the most important intracellular processes regarding the cellular uptake of biologicals is the endolysosomal pathway. Sophisticated nanocarriers are developed to overcome a major hurdle, the endosomal entrapment, and delivering their cargo to the required site of action. In parallel, in vitro assays are established analyzing the performance of these nanocarriers. Among them, the release of the membrane‐impermeable dye calcein has become a popular and straightforward method. It is accessible for most researchers worldwide, allows for rapid conclusions about the release potential, and enables the study of release mechanisms. This review is intended to provide an overview and guidance for scientists applying the calcein release assay. It comprises a survey of several applications in the study of endosomal escape, considerations of potential pitfalls, challenges, and limitations of the assay, and a brief summary of complementary methods. Based on this review, it is hoped to encourage further research groups to take advantage of the calcein release assay for their own purposes and help to create a database for more efficient cross‐correlations between nanocarriers.

show abstract

Section: Calcein Leakage From Artificial Model Vesiclesmentioning

confidence: 99%

Section: Calcein Leakage From Artificial Model Vesiclesmentioning

confidence: 99%

Tracking the Endosomal Escape: A Closer Look at Calcein and Related Reporters

Hausig‐Punke

Richter

Hoernke

et al. 2022

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…[20] When adding the acid or alkali for switching the wettability of the surface, most of the reagent doesn't work but only this at the interface acts as the inducer for the protonation of charged groups to change the wettability. [21] Auinger et al [22] reported that during electrochemical reactions, the produces or consumes H + or OH − at the solid-liquid interface can cause a significant change in the pH of the interface region. But the current methods of directly creating acidic or alkaline conditions need further exploration.…”

Section: Introductionmentioning

confidence: 99%

Constructing Scalable Membrane with Tunable Wettability by Electrolysis‐Induced Interface pH for Oil–Water Separation

Gan,

Li,

Zhu

et al. 2023

Adv Funct Materials

View full text Add to dashboard Cite

The tunable wettability by pH‐stimulus has great potential in liquid adhesion, transport, collection, and separation due to its rapid response and wide control range. However, achieving pH‐regulated wettability on the selected region of material without acid–base contamination presents a distinct challenge for the existing methods. Here, a scalable conductive network membrane is prepared with switchable wettability by regulating interfacial pH. The generation and diffusion of interfacial pH on the selected region of the membrane are regulated through the confinement electrolysis process, which is adapted to both spatial arrangements of the conductive network and the electrical potential. By regulating the interfacial pH (>13), the wettability of the selected region can change from superhydrophobicity (Water contact angle = 150°) to superhydrophilicity/underwater superoleophobicity (Water contact angle = 0°) without additional reagent in 30 s under 15 V. Based on the switchable wettability and precise controllability, the prepared membrane can efficiently realize on‐demand oil–water separation (>99%) and in situ extraction‐back extraction. The membrane with switchable wettability is programable and free of acid–base contamination, which may have broad practical application potential in intelligent fluid‐related systems.

show abstract

Determination of divalent metal ion-regulated proton concentration and polarity at the interface of anionic phospholipid membranes

Mandal,

Roy,

Karmakar

et al. 2024

Soft Matter

View full text Add to dashboard Cite

show abstract

Protonation-induced pH increase at the triblock copolymer micelle interface for transient membrane permeability at neutral pH

Abstract: An increase of bulk-to-interface pH-deviation due to partially protonated interface of tri-block polymer micelle makes an acidic membrane interface transiently permeable.

Cited by 3 publications

References 50 publications

Tracking the Endosomal Escape: A Closer Look at Calcein and Related Reporters

Tracking the Endosomal Escape: A Closer Look at Calcein and Related Reporters

Constructing Scalable Membrane with Tunable Wettability by Electrolysis‐Induced Interface pH for Oil–Water Separation

Determination of divalent metal ion-regulated proton concentration and polarity at the interface of anionic phospholipid membranes

Contact Info

Product

Resources

About