Protoplast fusion betweenLentinula edodes andCoriolus versicolor

Abstract

Protoplast fusion between isoleucine-, arginine- and thymidine-requiring auxotroph (lle, Arg, Thy) ofLentinula edodes and arginine-requiring auxotroph (Arg) ofCoriolus versicolor has been achieved using 30% polyethylene glycol (M.W. 4000) in 10 mMCaCl(2)-glycine solution (pH 8.0). Fusion hybrids were selected in the 0.6 M sucrose supplemented minimal media on the basis of nutritional complementation with fusion frequency of 7.4x10(-6). The hybrids included both parental and non-parental types in colony morphology, growth rate and isozyme patterns. We succeeded inter-order protoplast fusion between the auxotrophs ofLentinula edodes and Coriolus versicolor overcoming the natural barriers of incompatibility. We examined the characteristics of the hybrids and clarified the fusion process using electron microscopy.

Transmission of Fusarium boothii Mycovirus via Protoplast Fusion Causes Hypovirulence in Other Phytopathogenic Fungi

“…The present study, however, required that the genetic background of the recipient isolates be maintained when protoplast fusion was used to transfer hypovirulent dsRNA mycovirus to the recipients. Protoplast fusion with specific selection marker(s) has proven to be a novel approach by which potential strains with desirable properties could be obtained with minimal disturbance to their genetic background and physiology [29]. The genetic variability of recipient isolates was studied by sequencing the TEF and histone H3 gene region and by AFLP analysis.…”

Two Novel Mycoviruses Related to Geminivirus Isolated from the Soil-Borne FungiMacrophomina Phaseolina(Tassi) Goid. andMucor RacemosusBull

“…For example, some hypovirulence-associated fungal viruses have been used for controlling the plant fungal disease chestnut blight (25). It has previously been reported that the vegetative incompatibility between two fungal strains mostly limits the mycoviruses transmission, and that the transmission mostly affects the fungal morphology and the hyphal cells (19,34). Yu et al (38) postulated that the extracted viral DNA isolated directly from the infected fungal mycelium was able to transfer to S. sclerotiorum protoplasts.…”

Interspecies hybridization between Auricularia cornea cv. Yu Muer and Auricularia heimuer cv. Bai Muer through protoplast fusion

“…In our experiment, we observed that the primordia of hybrids failed to differentiate into fruiting bodies. How to maintain the stability of heterokaryons is a crucial problem during the development of distant hybrids (Kim et al, 1997). Regardless of the genetic mechanism, when two distant parents undergo protoplast fusion, the resulting hybrids can offer a range of benefits.…”

“…Pegler and Coriolus versicolor (L.) Quél. (Kim et al, 1997). Somatic hybrids between Calocybe indica Purkay & A. Chandra and Pleurotus fiorida Singer showed a significant increase in bio-eficiency and γ-linoleic acid content (Chakraborty and Sikdar, 2010).…”