Protoplast Fusion in Bacillus and Its Consequences

Hotchkiss, Rollin D.; Gabor, Magda

doi:10.1016/b978-0-12-222702-8.50011-1

Cited by 13 publications

(11 citation statements)

References 49 publications

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“…In this type of analysis, according to Karmazyn-Cam pbell et al (1985), cell wall regeneration lowers the number of prototrophic clones obtainable after a fusion. This observation would be in agreement with the suggested role played by cell wall chromosome attachment sites (Hotchkiss and Gabor, 1985), which could also be responsible for some of the unexplained phenomena such as inactivation of one of the two chromosomes, and nonreciprocal recombination and segregation observed in protoplast fusion of the bacilli. After protoplast fusion, in fact, a high portion of the colonies regenerated in the absence of selective pressure carry both parental chromosomes (Hotchkiss and Gabor, 1980;Guillen et ai., 1983) and only one of them is in a transcriptionally active state.…”

Section: Protoplast Fusionsupporting

confidence: 87%

“…Also the analysis of the fusants may be complicated by several variables. The reader is referred to recent comprehensive reviews by Hopwood (1981) and by Hotchkiss and Gabor (1985) which analyze the methodology in depth. Only a brief description of the methodology and some of the inherent problems are referred to here.…”

Section: Protoplast Fusionmentioning

confidence: 99%

“…When counterselection is employed there are often biases (Hotchkiss and Gabor, 1985) due to not yet clarified metabolic effects. Direct selection, although useful in certain cases when a particular class of recombinants is desired, might lower the recovery efficiency of certain classes of fusants.…”

Section: Protoplast Fusionmentioning

confidence: 99%

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Bacillus

1989

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Section: Protoplast Fusionsupporting

confidence: 87%

Section: Protoplast Fusionmentioning

confidence: 99%

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Bacillus

1989

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“…Another possible parallel, but this time with a prokaryote system, has been found in diploid cells expressing only one progenitor phenotype recovered following protoplast fusion in Bacillus spp. Such non-complementing diploids have been explained in terms of inactivation of one entire genome (Hotchkiss & Gabor, 1985).…”

Section: Discussionmentioning

confidence: 99%

Complex outcome of reciprocal exchange of nuclear DNA between two members of the basidiomycete genus Stereum

Ainsworth¹,

Beeching

Broxholme

et al. 1992

Journal of General Microbiology

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Strains of the basidiomycete fungi Stereum hirsutum from the USSR and Sfereum complicatum from the eastern USA were interfertile, yielding viable, variable, meiotic progeny. However, interactions between paired homokaryotic mycelia of these species were unstable, yielding a uniform mycelial mat on the S complicafum side, but a macroscopically heterogeneous mycelium on the S hirsutum side. Hyphal tips excised from the interactions gave rise to colonies identifiable as one or other of the progenitor strains or two non-progenitor types (C-types and H-types). C-types were all morphologically similar to one another and resembled the S compIicQtunt progenitor except in being inaccessible to nuclear migration and in exhibiting a light-induced phenotypic switch. They could be isolated from both sides of interactions and were somatically compatible with one another. H-types were only isolated from the S. hirsutum side of interactions and had a range of morphologies, generally similar to the S. hirsutum progenitor. Pairings between H-types, and between H-types and C-types sometimes resulted in pigmented interaction zones due to somatic incompatibility. Single, dual and minisatellite DNA probes were used to investigate restriction fragment length polymorphisms in progenitor and derivative strains. Nuclear, but not mitochondrial DNA from both species was detected in all non-progenitor strains obtained from beyond the interaction interface. However, whereas S. complicatum-specific nuclear sequences were consistently present, the distribution of S. hirsutum-specific sequences varied, and was partly correlated with possession of H-type morphologies.

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“…It is also clear that DNA can sometimes exist in remarkable "archived" states [Hotchkiss and Gabor, 1985;Downs and Roth, 19871. We can expect some novel insights in these areas in the next few years.…”

Section: Past and Future: The Driving Forcesmentioning

confidence: 99%