1989
DOI: 10.1104/pp.91.1.13
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Protoplast Volume:Water Potential Relationship and Bound Water Fraction in Spinach Leaves

Abstract: Methods used to estimate the (nonosmotic) bound water fraction (BWF) (i.e. apoplast water) of spinach (Spinacia oleracea L.) leaves were evaluated. Studies using three different methods of pressure/volume (P/V) curve construction all resulted in a similar calculation of BWF; approximately 40%. The theoretically derived BWF, and the water potential (I,)/relative water content relationship established from P/V curves were used to establish the relationship between protoplast (i.e. symplast) volume and *w.Another… Show more

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Cited by 18 publications
(9 citation statements)
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“…The coefficient of determination of this relationship was 0.49 and hence there are some other factors that will influence the magnitude of dilution. Species can vary in the amount of apoplastic water that is extracted during sample preparation because of a number of issues (Santakumari and Berkowitz , Urban et al , Callister et al ). Other variations in the π OSM can be introduced by possible variation among species in the amounts of ionic exchange between cell walls and solution (Tyree and Jarvis ), and in interaction between previously compartmentalized solutes in solution (Morgan , Marigo and Peltier ).…”
Section: Discussionmentioning
confidence: 99%
“…The coefficient of determination of this relationship was 0.49 and hence there are some other factors that will influence the magnitude of dilution. Species can vary in the amount of apoplastic water that is extracted during sample preparation because of a number of issues (Santakumari and Berkowitz , Urban et al , Callister et al ). Other variations in the π OSM can be introduced by possible variation among species in the amounts of ionic exchange between cell walls and solution (Tyree and Jarvis ), and in interaction between previously compartmentalized solutes in solution (Morgan , Marigo and Peltier ).…”
Section: Discussionmentioning
confidence: 99%
“…Third, the exponential coefficient relating ε to Ψ p (β) was estimated as −2.25, which allowed ε to approach within ≈ 10% of ε max at a Ψ p of 1.0 MPa; this was derived by inspection of curves presented in Jones et al (1985) and Cheung et al (1976). Fourth, an apoplastic water content of 30% of relative water content (RWC) was used, which was a compromise between values of 40% (Santakumari and Berkowitz 1989) and 15--20% (Hardegree 1989) obtained for drought-stressed spinach leaves, and close to the value of 31% obtained for droughtstressed orange leaves (Fereres et al 1979). Fifth, the slope of the linear portion of the PV curve (RWC versus Ψ π −1 ) was calculated assuming that Ψ π −1 approaches zero when the RWC reaches 30% (i.e., Ψ π approaches infinity when the symplastic water content reaches zero), and Ψ π −1 at 100% RWC equaled the reciprocal of Ψ π,o measurements for each species.…”
Section: Analysis Of Offsetting Effectsmentioning
confidence: 99%
“…The disadvantages ofthis technique have been pointed out (7,15), but a recent comparison of three different techniques of PV curve construction resulted in similar BW estimates (14). ' This paper is dedicated to the memory of Prof. G. Wittmer, director of the Experimental Institute for Cereal Research, Section of Foggia, who died in the summer of 1990.…”
mentioning
confidence: 99%