Protoplasts of Laminaria digitata and Laminaria saccharina (Phaeophyta) — Cultivation and Biosynthesis of Alginate

Abstract

Protoplasts have been prepared from sporophytes of Laminaria digitala and Laminaria saccharina. Different culture media have been tested in order to achieve cell wall regeneration. The most appropriate culture medium for Laminaria digitata protoplasts was found to be an ionic medium with glucose as extra osmoticum. For Laminaria saccharina protoplasts, seawater supplied with glucose was more appropriate. The composition of alginates produced from regenerating protoplasts have determined by NMR and by an enzymatic method. Alginate composition is found to be markedly influenced by the medium composition.

Isolation of protoplasts from Fucus serratus and F. vesiculosus(Fucales, Phaeophyceae): factors affecting protoplast yield

“…(Ducreux & Kloareg, 1988) and Pilayella littoralis (Mejjad et al, 1992), or economically valuable species as Undaria (Fujita & Migita, 1985;Tokuda & Kawashima, 1988;Wakabayashi et al, 1999;Matsumura et al, 2001), Laminaria species (Saga & Sakai, 1984;Butler et al, 1989;Sawabe & Ezura, 1996;Benet et al, 1997;Hagen Rodde & Larsen, 1997;Sawabe et al, 1997;Matsumura, 1998) and Macrocystis pyrifera Saga et al, 1986;Kloareg et al, 1989;Kevekordes et al, 1993).…”