Providencia entomophila sp. nov., a new bacterial species associated with major olive pests in Tunisia

Abstract

Bioprospection for potential microbial biocontrol agents associated with three major insect pests of economic relevance for olive cultivation in the Mediterranean area, namely the olive fly, Bactrocera oleae, the olive moth, Prays oleae, and the olive psyllid, Euphyllura olivina, led to the isolation of several strains of readily cultivable Gram-negative, rod-shaped bacteria from Tunisian olive orchards. Determination of 16S ribosomal RNA encoding sequences identified the bacteria as members of the taxonomic genus Providencia (Enterobacterales; Morganellaceae). A more detailed molecular taxonomic analysis based on a previously established set of protein-encoding marker genes together with DNA-DNA hybridization and metabolic profiling studies led to the conclusion that the new isolates should be organized in a new species within this genus. With reference to their original insect association, the designation “Providencia entomophila” is proposed here for this hypothetical new taxon.

Gut Bacteriome Analysis of Anastrepha fraterculus sp. 1 During the Early Steps of Laboratory Colonization

“…The most abundant genera, were Wolbachia (Alphaproteobacteria) and Enterobacter (Gammaproteobacteria), followed by other members of the Gammaproteobacteria such as Providencia, Aeromonas, Citrobacter, Burkholderia-Caballeronia-Paraburkholderia, Klebsiella and Raoultella. Tephritid literature reports these genera of Proteobacteria with specific functions: Wolbachia with cytoplasmic incompatibility and male killing (Boller and Bush, 1974;Riegler and Stauffer, 2002;Zabalou et al, 2004Zabalou et al, , 2009Apostolaki et al, 2011;Conte et al, 2019;Devescovi et al, 2019;Mateos et al, 2020), Enterobacter, Citrobacter, Burkholderia, Klebsiella and Raoultella with nitrogen metabolism (Murphy et al, 1988;Behar et al, 2005;Raza et al, 2020), and Providencia with pathogenic effects (Msaad Guerfali et al, 2018;Ksentini et al, 2019). In addition, Acinetobacter and Rheinheimera (Gammaproteobacteria) were detected in low abundance (0,1% of total reads) but in a high percentage of samples (33.33 and 46.97% of total samples, respectively) and wide distribution (Lab, WU, F0, F1-F6 flies).…”

Bacillus velezensis strain MBY2, a potential agent for the management of crown gall disease

“…Moreover, for comprehensive analysis of the information content of the four protein-encoding genes, a codon-aligned concatenation of the MLSA marker sequences was created. The Tree-Puzzle 5.2 software [ 29 ] was used to estimate data set specific parameters as nucleotide frequencies, the percentage of invariable sites, the transition/transversion ratio and the alpha-parameter for the gamma-distribution-based correction of rate heterogeneity among sites [ 30 ].…”

“…Phylogenies were reconstructed i) from p-distance matrices with the Neighbor Joining (NJ) method as implemented in the MEGA 6 software tool under pair-wise deletion of alignment gaps and missing data or ii) using the Maximum Likelihood (ML) method as implemented in the PhyML software tool [ 32 ] using the Hasegawa-Kishino-Yano (HKY) model of nucleotide substitution [ 33 ] under the assumption of a gamma-distribution-based model of rate heterogeneity [ 34 ] allowing for eight rate categories. Tree topology confidence limits were explored in non-parametric bootstrap analyses over 1,000 pseudo-replicates [ 30 ].…”

“…For the double layer method, a suspension of 10 7 CFU ml -1 of the bacterial isolate to be tested was spot-inoculated onto LB medium. After 48h of incubation at 25˚C, the potentially antagonistic strain was exposed to chloroform vapor for 30 left open for 15 min in a laminar flow cabinet. One milliliter of A. fabrum C58 suspension (10 7 CFU ml -1 ) was mixed with 3 ml of LBA (0.6% agar) at 45˚C and quickly inoculated by spreading on the plates containing the isolate to be tested.…”

Comparative Genomic Analysis Reveals Genetic Mechanisms of the Variety of Pathogenicity, Antibiotic Resistance, and Environmental Adaptation of Providencia Genus

“…Providencia isolated from human stool samples are often thought of as part of the natural human gut flora or the cause of gastric disturbances ("travelers' diarrhea") (Ryan et al, 1992;Sobreira et al, 2001). According to previous reports, Providencia could sub-divided into 10 recognized species, Providencia alcalifaciens, Providencia burhodogranariea, Providencia heimbachae, Providencia rettgeri, Providencia rustigianii, Providencia sneebia, Providencia stuartii, Providencia thailandensis, Providencia vermicola, and Providencia huaxiensis (Penner and Hennessy, 1979;Hickman-Brenner et al, 1983;Yoh et al, 2005;Somvanshi et al, 2006;Juneja and Lazzaro, 2009;Khunthongpan et al, 2013;Jneid et al, 2016;Ksentini et al, 2019).…”