2024
DOI: 10.1101/2024.10.11.617810
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Provirus deletion fromHaloferax volcaniiaffects motility, stress resistance and CRISPR RNA expression

Nadia Di Cianni,
Simon Bolsinger,
Jutta Brendel
et al.

Abstract: Haloferax volcaniiharbours four putative proviruses: Halfvol1, Halfvol2, Halfvol3 and Halfvol4. In this study we successfully deleted all four provirus genomes, demonstrating, that they are not essential. Transcriptome comparison between this strain (ΔHalfvol1-4) and a wild type strain reveals an increase in archaella and chemotaxis gene expression, resulting in higher swarming motility in ΔHalfvol1-4. Furthermore, ΔHalfvol1-4 cells show an elongated cell shape and a higher resistance to H2O2stress compared to… Show more

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