Proximate, amino acid and lipid compositions in <i>Sinonovacula constricta</i> (Lamarck) reared at different salinities

Ran, Zhaoshou; Li, Shuang; Zhang, Runtao; Xu, Jilin; Liao, Kai; Yu, Xiaoyan; Zhong, Yingying; Ye, Mengwei; Yu, Shanshan; Ran, Yun; Huang, Wei; Yan, Xiaojun

doi:10.1002/jsfa.8311

Cited by 34 publications

(16 citation statements)

References 31 publications

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“…Specifically, as one of the five major bivalves in the world's aquaculture industry, S. constricta's global production exceeds 823,000 tons, with a market value of US$ 1.3 billion in 2016 (FAO, ). The meat of S. constricta possesses high levels of n − 3 long‐chain polyunsaturated fatty acids, especially docosahexaenoic acid, which accounts for about 10% of the total fatty acids (Ran, Chen, et al, ; Ran, Li, et al, ). Moreover, S. constricta can drill a depth of 20 cm, changing the benthic oxygen environment, which is conducive to the aquatic ecology (Lukwambe et al, ).…”

Section: Introductionmentioning

confidence: 99%

Chromosome‐level genome assembly of the razor clam Sinonovacula constricta (Lamarck, 1818)

Ran

Yan

et al. 2019

Molecular Ecology Resources

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Bivalves, a highly diverse and the most evolutionarily successful class of invertebrates native to aquatic habitats, provide valuable molecular resources for understanding the evolutionary adaptation and aquatic ecology. Here, we reported a high‐quality chromosome‐level genome assembly of the razor clam Sinonovacula constricta using Pacific Bioscience single‐molecule real‐time sequencing, Illumina paired‐end sequencing, 10X Genomics linked‐reads and Hi‐C reads. The genome size was 1,220.85 Mb, containing scaffold N50 of 65.93 Mb and contig N50 of 976.94 Kb. A total of 899 complete (91.92%) and seven partial (0.72%) matches of the 978 metazoa Benchmarking Universal Single‐Copy Orthologs were determined in this genome assembly. And Hi‐C scaffolding of the genome resulted in 19 pseudochromosomes. A total of 28,594 protein‐coding genes were predicted in the S. constricta genome, of which 25,413 genes (88.88%) were functionally annotated. In addition, 39.79% of the assembled genome was composed of repetitive sequences, and 4,372 noncoding RNAs were identified. The enrichment analyses of the significantly expanded and contracted genes suggested an evolutionary adaptation of S. constricta to highly stressful living environments. In summary, the genomic resources generated in this work not only provide a valuable reference genome for investigating the molecular mechanisms of S. constricta biological functions and evolutionary adaptation, but also facilitate its genetic improvement and disease treatment. Meanwhile, the obtained genome greatly improves our understanding of the genetics of molluscs and their comparative evolution.

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Section: Introductionmentioning

confidence: 99%

Chromosome‐level genome assembly of the razor clam Sinonovacula constricta (Lamarck, 1818)

Ran

Yan

et al. 2019

Molecular Ecology Resources

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“…Moreover, the flavour quality of aquatic organisms is mainly attributed to the content of flavour AA comprising the muscles. Aspartic and glutamic acids are characteristic flavour AAs, and the glycine, alanine, proline and serine also belong to flavour AAs (Ran et al, ). In the present study, the content of flavour AA reached the highest in clams fed with Undaria + Tetraselmis diet, and the proportion of these six flavour AAs in total free AA was equivalent to that in clams fed with the Tetraselmis diet (Table ).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, most seaweeds contain abundant proteins and highly unsaturated fatty acids (HUFAs), for example eicosapentaenoic acid (EPA) and DHA (Fleurence, ; McDermid & Stuercke, ; Su, Liu, & Chein, ; Tao & He, ). Amino acids (AAs) play a vital role in food nutrition and flavour of bivalves (Ran et al, ), and HUFAs are essential for the normal development, survival and growth of bivalves (Tanyaros & Chuseingjaw, ). These findings implicate the essential roles of macroalgal detritus as potential food sources for the Manila clam.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of macroalgal detritus as food source for juvenile Manila clam, Ruditapes philippinarum : Effects on growth, amino acid content and fatty acid composition

et al. 2019

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Sufficient high‐quality microalgae are required for indoor nursery of juvenile Ruditapes philippinarum. However, culturing numerous microalgae to support clam feeding is a heavy burden on many hatcheries. The effects of detritus from the macroalgae Ulva pertusa, Chondrus ocellatus and Undaria pinnatifida on the growth, amino acid content and fatty acid profile of R. philippinarum were assessed as potential substitute diets. The green microalga Tetraselmis cordiformis served as comparative diet. Results revealed that the clams ingesting distinct diets presented no significant differences in growth of soft tissues, but the nutritional component of these clams differed dramatically. The clams fed with Undaria + Tetraselmis had the highest content of essential amino acids and proteins. In addition, the clams fed with single macroalgal diets and mixed macroalgal detritus and Tetraselmis showed significantly higher or statistically equal levels in n‐3/n‐6 ratio and docosahexaenoic acid (DHA)/eicosapentaenoic acid (EPA) ratio with respect to Tetraselmis diets. The relative percentages of EPA and DHA in clams fed with Undaria were 28% and 63% higher than those fed with Tetraselmis, and the arachidonic acid abundances in clams ingesting Undaria + Tetraselmis and Tetraselmis were significantly higher than those in clams ingesting other diets. Together, the diets containing single Undaria or mixed Undaria + Tetraselmis produced Manila clams with nutritional advantages in terms of essential amino acids and polyunsaturated fatty acids. Thus, the detritus of macroalgae, especially Undaria, is an appropriate substitute diet, at least partially, for culture of nutrition‐improved R. philippinarum.

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“…Although the razor clam can withstand salinity stress to some extent, the acute and dramatic increase in salinity often leads to large-scale mortality of individuals (Li et al, 2021). On the other hand, studies suggest that aquatic animals cultured under higher salinity often have better taste and nutrition (Ran et al, 2017;Kumari et al, 2021;Luo et al, 2021), which promotes the breeding of new varieties with high salinity tolerance. However, information on the response of S. constricta to high salinity stress is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Effects of High Salinity Stress on the Survival, Gill Tissue, Enzyme Activity and Free Amino Acid Content in Razor Clam Sinonovacula constricta

Cao

Chi

et al. 2022

Front. Mar. Sci.

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Salinity can affect the physiological and productive characteristics of bivalves. The razor clam Sinonovacula constricta naturally distributing in intertidal zones and estuarine waters is extensively cultured in China. High-salinity is a serious threat to its culture, especially in pond culture model. Here, we investigated the effects of high-salinity on the survival, gill tissue, enzyme activity and free amino acid content of S. constricta. Individuals were randomly divided into six groups treated with different salinities of 20, 25, 30, 35, 40, and 45 ppt. The median lethal salinity calculated by linear regression and probit analysis methods were similar, with 46.61, 43.17, 36.99, 35.99, and 34.99 ppt by linear regression, while 44.63, 42.87, 38.45, 37.32, and 36.17 ppt by probit analysis at 72, 96, 120, 144, and 168 h, respectively. Furthermore, paraffin section revealed that the gill of razor clam shrunk quickly under high-salinity and dissolved at 48 h under salinity of 40 ppt, but not under 30 ppt. In addition, the activities of three enzymes including Na+-K+-ATPase, acid phosphatase and alkaline phosphatase changed significantly under high-salinity. Besides, the contents of total free amino acids (TFAAs) in the gill tissues were 61.25 ± 1.58, 89.36 ± 4.18, and 111.98 ± 1.46 mg⋅g–1 at salinity of 20, 30, and 40 ppt, respectively, indicating increasing TFAA contents with salinity. Notably, several amino acid species including taurine, alanine, proline, glycine and glutamic showed significantly changed contents in response to salinity variation, suggesting these amino acids might play an important role in razor clam’s adaptation to salinity stress. Combining all these results, it was referred that razor clams may adapt to moderately high-salinity of 30 ppt in a short time through osmotic adjustment. Although a small proportion of individuals could survive under the salinity of 35 ppt, the death of most individuals still suggested it can hardly be accepted in aquacultural production. Notably, living under a salinity of 40 ppt for more than 48 h could cause great damage to razor clams. Therefore, the seawater salinity is recommended to be lower than 30 ppt for S. constricta aquaculture. Overall, this study provides guidance for the culture and breeding of high-salinity tolerant razor clams.

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Proximate, amino acid and lipid compositions in Sinonovacula constricta (Lamarck) reared at different salinities

Cited by 34 publications

References 31 publications

Chromosome‐level genome assembly of the razor clam Sinonovacula constricta (Lamarck, 1818)

Chromosome‐level genome assembly of the razor clam Sinonovacula constricta (Lamarck, 1818)

Evaluation of macroalgal detritus as food source for juvenile Manila clam, Ruditapes philippinarum : Effects on growth, amino acid content and fatty acid composition

Effects of High Salinity Stress on the Survival, Gill Tissue, Enzyme Activity and Free Amino Acid Content in Razor Clam Sinonovacula constricta

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