Proximity-driven bioorthogonal reactions<i>via</i>DNA circuits enable the accurate aptasensing of non-nucleic acid targets

Zhao, Su-Jing; Guo, Haiyu; Wu, Zhenkun; Jiang, Jian‐Hui

doi:10.1039/d2cc05628c

Chem. Commun.

2022

DOI: 10.1039/d2cc05628c

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Proximity-driven bioorthogonal reactionsviaDNA circuits enable the accurate aptasensing of non-nucleic acid targets

Su-Jing Zhao

Haiyu Guo

Zhenkun Wu

et al.

Abstract: Tetrazine-mediated bioorthogonal reactions was rationally coupled with DNA cascade circuits to enable proximal decaging, which allowed the construction of a fluorogenic aptasensor for the accurate and amplified sensing of non-nucleic...

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Tumor Microenvironment‐Responsive Hydrogel for Direct Extracellular ATP Imaging‐Guided Surgical Resection with Clear Boundaries

Yang

Teng

et al. 2023

Adv Healthcare Materials

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Most solid tumors are clinically treated using surgical resection, and the presence of residual tumor tissues at the surgical margins often determines tumor survival and recurrence. Herein, a hydrogel (Apt‐HEX/Cp‐BHQ1 Gel, termed AHB Gel) is developed for fluorescence‐guided surgical resection. AHB Gel is constructed by tethering a polyacrylamide hydrogel and ATP‐responsive aptamers together. It exhibits strong fluorescence under high ATP concentrations corresponding to the TME (100–500 µm) but shows little fluorescence at low ATP concentrations (10–100 nm) such as those in normal tissues. AHB Gel can rapidly (within 3 min) emit fluorescence after exposure to ATP, and the fluorescence signal only occurs at sites exposed to high ATP, resulting in a clear boundary between the ATP‐high and ATP‐low regions. In vivo, AHB Gel exhibits specific tumor‐targeting capacity with no fluorescence response in normal tissue, providing clear tumor boundaries. In addition, AHB Gel has good storage stability, which is conducive to its future clinical application. In summary, AHB Gel is a novel tumor microenvironment‐targeted DNA‐hybrid hydrogel for ATP‐based fluorescence imaging. It can enable the precise imaging of tumor tissues, showing promising application in fluorescence‐guided surgeries in the future.

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Tumor Microenvironment‐Responsive Hydrogel for Direct Extracellular ATP Imaging‐Guided Surgical Resection with Clear Boundaries

Yang

Teng

et al. 2023

Adv Healthcare Materials

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Proximity-driven bioorthogonal reactionsviaDNA circuits enable the accurate aptasensing of non-nucleic acid targets

Abstract: Tetrazine-mediated bioorthogonal reactions was rationally coupled with DNA cascade circuits to enable proximal decaging, which allowed the construction of a fluorogenic aptasensor for the accurate and amplified sensing of non-nucleic...

Cited by 1 publication

References 31 publications

Tumor Microenvironment‐Responsive Hydrogel for Direct Extracellular ATP Imaging‐Guided Surgical Resection with Clear Boundaries

Tumor Microenvironment‐Responsive Hydrogel for Direct Extracellular ATP Imaging‐Guided Surgical Resection with Clear Boundaries

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