Proximity hybridization triggered hybridization chain reaction for label-free electrochemical homogeneous aptasensors

Liao, Xin; Zhang, Caiyi; Machuki, Jeremiah Ong’achwa; Wen, Xiaoqing; Chen, Duankai; Tang, Qianli; Gao, Fenglei

doi:10.1016/j.talanta.2020.122058

Cited by 22 publications

(5 citation statements)

References 52 publications

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“…The linear response range and LOD for CCRF-CEM sEVs is 4 × 10 3 to 8 × 10 7 particles/μL and 920 particles/μL, respectively, indicating potential application in accurate cancer diagnostics. Utilizing hybridization chain reaction amplification, a wide detection range for the detection of ATP (4 to 4 × 10 5 U/L), exosomes (5 × 10 2 to 1 × 10 5 exosomes/μL), thrombin (0.1 pM to 100 nM), lysozyme (10 –10 M to 10 –5 M), and HepG2 cells (10 2 to 10 7 cells/ml) can be obtained. Employing rolling circle amplification, wide detection ranges of circulating tumor cells (5 to 3 × 10 4 cells mL –1 ), lipopolysaccharide (0.01 pg mL –1 to 100 ng mL –1 ), platelet-derived growth factor B-chain (10 fM to 100 pM), prostate-specific antigen (0.05–500 fg/mL), HepG2 tumor cells (10 to 10 6 cells/mL), protein PDGF-BB (8.4 × 10 –11 to 8.4 × 10 –9 M), and breast cancer MCF-7 cells (20–5 × 10 6 cells/mL) were also realized.…”

Section: Aptamer Engineeringmentioning

confidence: 99%

Electrochemical Aptamer-Based Sensors with Tunable Detection Range

Pan

et al. 2023

Anal. Chem.

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Section: Aptamer Engineeringmentioning

confidence: 99%

Electrochemical Aptamer-Based Sensors with Tunable Detection Range

Pan

et al. 2023

Anal. Chem.

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“…84−86 For example, Liao et al designed a homogeneous aptasensor for thrombin assay utilizing proximity hybridization leaded HCR. 87 Multiple Gquadruplex formed which captured the electrochemical indicator, hemin, resulting in significant variation of electrochemical response. We took advantage of the significant repellent feature of [Fe(CN) 6 ] 3−/4− and negatively charged HCR product in an aptasensor that monitored arsenic contamination.…”

Section: Hcr Amplified Signalsmentioning

confidence: 99%

“…Electrochemical species are intermediate mediums between electrode and target, which transmit electrical and chemical information . Various electrochemical species with distinct signatures have been studied and applied including hexaammineruthenium(III) chloride ([Ru(NH 3 ) 6 ] 3+ ), methylene blue (MB), ferrocene (Fc), hemin, and potassium ferricyanide ([Fe(CN) 6 ] 3– ). − For example, Liao et al designed a homogeneous aptasensor for thrombin assay utilizing proximity hybridization leaded HCR . Multiple G-quadruplex formed which captured the electrochemical indicator, hemin, resulting in significant variation of electrochemical response.…”

Section: Hcr Amplified Signalsmentioning

confidence: 99%

Recent Progress in DNA Hybridization Chain Reaction Strategies for Amplified Biosensing

Chai

Cheng

Jin

et al. 2021

ACS Appl. Mater. Interfaces

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With the continuous development of DNA nanotechnology, various spatial DNA structures and assembly techniques emerge. Hybridization chain reaction (HCR) is a typical example with exciting features and bright prospects in biosensing, which has been intensively investigated in the past decade. In this Spotlight on Applications, we summarize the assembly principles of conventional HCR and some novel forms of linear/nonlinear HCR. With advantages like great assembly kinetics, facile operation, and an enzyme-free and isothermal reaction, these strategies can be integrated with most mainstream reporters (e.g., fluorescence, electrochemistry, and colorimetry) for the ultrasensitive detection of abundant targets. Particularly, we select several representative studies to better illustrate the novel ideas and performances of HCR strategies. Theoretical and practical utilities are confirmed for a range of biosensing applications. In the end, a deep discussion is provided about the challenges and future tasks of this field.

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“…In addition, probes situated in the same plane may face limitations due to steric hindrance, which subsequently decreases the efectiveness of recognition and binding. By contrast, homogeneous electrochemical biosensors, whose recognition and response processes occur in the solution phase instead of on the electrode surface, avoid complicated immobilization processing [26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Label-Free Ratiometric Homogeneous Electrochemical Strategy Based on Exonuclease III-Aided Signal Amplification for Facile and Rapid Detection of miR-378

Fan,

Wang,

Wang

et al. 2024

International Journal of Analytical Chemistry

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MiR-378 is abnormally expressed in various cancers, such as hepatocellular carcinoma, renal cell carcinoma, and nonsmall cell lung cancer. Here, we developed a label- and immobilization-free ratiometric homogeneous electrochemical strategy based on exonuclease III (Exo III) for the facile and rapid determination of miR-378. Two 3′-protruding hairpin DNA probes (HPs) are designed in this strategy. Doxorubicin (DOX) and potassium ferrocyanide (Fe2+) were used as label-free probes to produce a response signal (IDOX) and a reference signal (IFe2+) in the solution phase. When no target was present in the solution, the HP was stable, most of the DOX was intercalated in the stem of the HP, and the diffusion rate of DOX was significantly reduced, resulting in reduced electrochemical signal response. When miR-378 was present, double-cycle signal amplification triggered by Exo III cleavage was initiated, ultimately disrupting the hairpin structures of HP1 and HP2 and releasing a large amount of DOX into the solution, yielding a stronger electrochemical signal, which was low to 50 pM. This detection possesses excellent selectivity, demonstrating high application potential in biological systems, and offers simple and low-cost electrochemical detection for miR-378.

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Proximity hybridization triggered hybridization chain reaction for label-free electrochemical homogeneous aptasensors

Cited by 22 publications

References 52 publications

Electrochemical Aptamer-Based Sensors with Tunable Detection Range

Electrochemical Aptamer-Based Sensors with Tunable Detection Range

Recent Progress in DNA Hybridization Chain Reaction Strategies for Amplified Biosensing

Label-Free Ratiometric Homogeneous Electrochemical Strategy Based on Exonuclease III-Aided Signal Amplification for Facile and Rapid Detection of miR-378

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