PRP in wound healing applications

Oneto, Paula; Etulain, Julia

doi:10.1080/09537104.2020.1849605

Cited by 55 publications

(46 citation statements)

References 152 publications

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“…Research on the impaired healing process is proceeding hastily as evidenced by new therapeutic approaches other than conventional, such as single growth factor, dual growth factors, skin substitutes, cytokine stimulators, cytokine inhibitors, matrix metalloproteinase inhibitors, gene and stem cell therapy, and extracellular matrix and angiogenesis stimulators. Regenerative medicine has emerged as an alternative therapeutic option to improve wound healing and restore normal skin architecture [32], in addition to the therapeutic use of platelet-rich plasma (PRP) and its derivatives [33] and stem cellbased therapy, which has become a promising new approach in the field of regenerative medicine [34]. In each of these approaches, the role of growth factors in the complex and overlapping phases of wound healing has been studied consistently over time [35][36][37].…”

Section: Discussionmentioning

confidence: 99%

“…Lipoaspirate was obtained from five healthy female patients (age range [30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45] undergoing an elective liposuction from a single anatomical site (abdominal subcutaneous fat tissue) at the Image Regenerative Clinic, Milan, Italy. Exclusion criteria were body mass index (BMI) > 30, diabetes, hypertension, and nicotine or alcohol abuse.…”

Section: Preparation Of Lipoaspiratementioning

confidence: 99%

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Lipoaspirate Shows In Vitro Potential for Wound Healing

et al. 2022

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Mesenchymal stem cells (MSCs) are a promising therapy in wound healing, although extensive time and manipulation are necessary for their use. In our previous study on cartilage regeneration, we demonstrated that lipoaspirate acts as a natural scaffold for MSCs and gives rise to their spontaneous outgrowth, together with a paracrine effect on resident cells that overcome the limitations connected to MSC use. In this study, we aimed to investigate in vitro whether the microfragmented adipose tissue (lipoaspirate), obtained with Lipogems® technology, could promote and accelerate wound healing. We showed the ability of resident cells to outgrow from the clusters of lipoaspirate encapsulated in a 3D collagen substrate as capability of repopulating a culture of human skin. Moreover, we demonstrated that the in vitro lipoaspirate paracrine effect on fibroblasts and keratinocytes proliferation, migration, and contraction rate is mediated by the release of trophic/reparative proteins. Finally, an analysis of the paracrine antibacterial effect of lipoaspirate proved its ability to secrete antibacterial factors and its ability to modulate their secretion in culture media based on a bacterial stimulus. The results suggest that lipoaspirate may be a promising approach in wound healing showing in vitro regenerative and antibacterial activities that could improve current therapeutic strategies.

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Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Lipoaspiratementioning

confidence: 99%

Lipoaspirate Shows In Vitro Potential for Wound Healing

et al. 2022

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“…However, there is a risk of the development of neuroarthropathy after using high doses of anti-NGF antibodies [24]. In addition, the regenerative capacity of the nervous system is limited, increasing preclinical evidence indicates that PRP would contribute to promoting the repair of this tissue [25].…”

Section: Oa and Painmentioning

confidence: 99%

Overview of First-Line and Second-Line Pharmacotherapies for Osteoarthritis with Special Focus on Intra-Articular Treatment

Nowaczyk

Szwedowski

Dallo³

et al. 2022

IJMS

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Osteoarthritis (OA) can be defined as the result of pathological processes of various etiologies leading to damage to the articular structures. Although the mechanism of degenerative changes has become better understood due to the plethora of biochemical and genetic studies, the drug that could stop the degenerative cascade is still unknown. All available forms of OA therapy are based on symptomatic treatment. According to actual guidelines, comprehensive treatment of OA should always include a combination of various therapeutic options aimed at common goals, which are pain relief in the first place, and then the improvement of function. Local treatment has become more common practice, which takes place between rehabilitation and pharmacological treatment in the hierarchy of procedures. Only in the case of no improvement and the presence of advanced lesions visible in imaging tests, should surgery be considered. Currently, an increasing number of studies are being published suggesting that intra-articular injections may be as effective or even more effective than non-steroidal anti-inflammatory drugs (NSAIDs) and result in fewer systemic adverse events. The most commonly used preparations are hyaluronic acid (HA), glucocorticosteroids (GS), and also platelet-rich plasma (PRP) in recent years. This review aims to present the mechanism of action and clinical effectiveness of different pharmacological options in relieving pain and improving functions in OA as well as the emerging approach in intra-articular treatment with PRP.

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“…5 On the other hand, plasma offers a fibrin scaffold for regeneration and controls the proliferative and oxidative homeostasis during wound healing. 5 In addition, and in the context of CHS, we have recently demonstrated that dampening haemoglobin oxidation (and the consequent tissue damage) by plasma is a possible new and non-canonical mechanism underlying the therapeutic effect of PRP in CHS. 6 In this sense, three different clinical groups including ours at the Hospital Fernández in Argentina, have reported that the intraarticular injection of PRP reduces the bleeding episodes in patients with CHS and improves clinical parameters of articular damage.…”

Section: Introductionmentioning

confidence: 99%

“…Alternatively, the intraarticular injection of blood‐derived autologous platelet rich‐plasma (PRP) has emerged as a novel, simple and accessible therapeutic approach for treating chronic haemophilic synovitis (CHS) as it was previously stablished for treating other several injuries and inflammatory degenerative diseases 4 . The therapeutic regenerative effect of PRP is due to the action of platelet‐derived growth factors and cytokines, which regulate the immune system and trigger cellular regenerative processes including (1) the revascularisation of damaged tissue; (2) the restoration of damaged connective tissue; and (3) the proliferation and differentiation of mesenchymal stem cells in tissue‐specific cells, allowing regeneration 5 . On the other hand, plasma offers a fibrin scaffold for regeneration and controls the proliferative and oxidative homeostasis during wound healing 5 .…”

Section: Introductionmentioning

confidence: 99%

DNA extracellular traps as potential biomarker of chronic haemophilic synovitis and therapeutic perspective in patients treated with PRP: A pilot study

et al. 2022

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Introduction Hemarthrosis causes chronic haemophilic synovitis (CHS). Although neutrophils are major immune cells infiltrating joints after bleeding, their role on the pathogenesis of CHS is unknown. Neutrophils release extracellular DNA traps (ETs), structures of DNA with bound granular enzymes that were associated with tissue damage. Aims To evaluate the presence of ETs as pathogenic biomarker and the protective effect of intraarticular injection of platelet‐rich plasma (PRP) in patients with CHS. Methods Haemophilia Joint Health Score (HJHS) and bleeding episodes (BE) were measured and correlated with ETs indicators (DNA/DNA‐Elastase) in synovial fluids (SF), PRP and plasma of 21 patients. Results Soluble DNA and DNA‐Elastase were detected in SF and plasma of patients. The synovial and plasma levels of DNA‐Elastase positively correlated with worse HJHS/BE. Interestingly, remaining ETs‐inducer factors were present in SF that induced the in vitro release of ETs from blood‐isolated neutrophils. This phenomenon was impaired by adding plasma or PRP. Finally, preliminary data obtained from five patients indicate that levels of DNA‐Elastase and HJHS/BE decreased after receiving intraarticular injection of PRP. Conclusions The synovial and plasma levels of DNA‐Elastase correlated with worse HJHS/BE suggesting that ETs formation could be a biomarker and potential therapeutic target for CHS. The intraarticular injection of PRP underlined a new potential alternative therapy, decreasing ETs formation in synovia of patients with CHS. However, our hypotheses must be confirmed in the future with better designed and more statistical power studies. Meanwhile, the use of intraarticular injections of PRP for the treatment of CHS remains controversial.

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PRP in wound healing applications

Cited by 55 publications

References 152 publications

Lipoaspirate Shows In Vitro Potential for Wound Healing

Lipoaspirate Shows In Vitro Potential for Wound Healing

Overview of First-Line and Second-Line Pharmacotherapies for Osteoarthritis with Special Focus on Intra-Articular Treatment

DNA extracellular traps as potential biomarker of chronic haemophilic synovitis and therapeutic perspective in patients treated with PRP: A pilot study

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